Mouse Anti-CTH Recombinant Antibody (CBXC-1973) (V2LY-0125-LY258)

Name: Mouse Anti-CTH Recombinant Antibody (CBXC-1973)
SKU: V2LY-0125-LY258
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CTH Recombinant Antibody (CBXC-1973) (V2LY-0125-LY258) in WB

Expressions of the (A) CTH expression, (B) CBS expression, and (C) H2S concentration. ...View More

Pérez-Torres, I., Manzano-Pech, L., Guarner-Lans, V., Soto, M. E., Castrejón-Téllez, V., Márquez-Velasco, R., ... & Torres-Narváez, J. C. (2022). Deodorized garlic decreases oxidative stress caused by lipopolysaccharide in rat heart through hydrogen sulfide: Preliminary Findings. International Journal of Molecular Sciences, 23(20), 12529. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CTH Recombinant Antibody (CBXC-1973) (V2LY-0125-LY258) in WB

The expression of TST, MPST, CBS, and CTH on the protein level in leukemia cell lines (Western blot analysis). β-Actin was used as the loading control. Data from a representative experiment are presented. ...View More

Jurkowska, H., Wróbel, M., Jasek-Gajda, E., & Rydz, L. (2022). Sulfurtransferases and cystathionine beta-synthase expression in different human leukemia cell lines. Biomolecules, 12(2), 148. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CTH Recombinant Antibody (CBXC-1973) (V2LY-0125-LY258) in WB

Primary mice cardiomyocytes were treated with vehicle or SPRC (25, 50, and 100 μM) for 24 h. The expression level of CSE were determined by western blotting (n = 3). **P < 0.01 vs. Vehicle group. ...View More

Li, Y., Xie, K. F., Chang, Y. H., Wang, C., Chen, Y., Wang, M. J., & Zhu, Y. C. (2021). S-Propargyl-cysteine attenuates diabetic cardiomyopathy in db/db mice through activation of cardiac insulin receptor signaling. Frontiers in Cardiovascular Medicine, 8, 737191. Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXC-1973

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 43-75 within an internal region of human CTH.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Cattle, Pig

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/mL

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Cystathionine Gamma-Lyase

Entrez Gene ID

Human	1491
Mouse	107869
Rat	24962

UniProt ID

Human	P32929
Mouse	Q8VCN5
Rat	P18757

Function

Catalyzes the last step in the trans-sulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure. Acts as a cysteine-protein sulfhydrase by mediating sulfhydration of target proteins: sulfhydration consists of converting -SH groups into -SSH on specific cysteine residues of target proteins such as GAPDH, PTPN1 and NF-kappa-B subunit RELA, thereby regulating their function.

Biological Process

Cellular response to leukemia inhibitory factor Source: Ensembl
Cysteine biosynthetic process Source: UniProtKB
Cysteine biosynthetic process via cystathionine Source: BHF-UCL
Cysteine metabolic process Source: ProtInc
Endoplasmic reticulum unfolded protein response Source: UniProtKB
Hydrogen sulfide biosynthetic process Source: UniProtKB
Negative regulation of apoptotic signaling pathway Source: Ensembl
Positive regulation of aortic smooth muscle cell differentiation Source: BHF-UCL
Positive regulation of I-kappaB kinase/NF-kappaB signaling Source: Ensembl
Positive regulation of NF-kappaB transcription factor activity Source: Ensembl
Protein homotetramerization Source: UniProtKB
Protein-pyridoxal-5-phosphate linkage via peptidyl-N6-pyridoxal phosphate-L-lysine Source: UniProtKB
Protein sulfhydration Source: UniProtKB
Sulfur amino acid catabolic process Source: Reactome
Transsulfuration Source: BHF-UCL

Cellular Location

Cytoplasm

Involvement in disease

Cystathioninuria (CSTNU):
Autosomal recessive phenotype characterized by abnormal accumulation of plasma cystathionine, leading to increased urinary excretion.

Lancien, M., Gueno, L., Salle, S., Merieau, E., Beriou, G., Nguyen, T. H., ... & Louvet, C. (2021). Cystathionine‐gamma‐lyase overexpression in T cells enhances antitumor effect independently of cysteine autonomy. Cancer Science, 112(5), 1723.

Xu, S., Pan, J., Cheng, X., Zheng, J., Wang, X., Guan, H., ... & Zhang, L. (2020). Diallyl trisulfide, a H2S donor, inhibits cell growth of human papillary thyroid carcinoma KTC‐1 cells through a positive feedback loop between H2S and cystathionine‐gamma‐lyase. Phytotherapy Research, 34(5), 1154-1165.

Chen, L., Lu, C., & Hua, Y. (2020). Cystathionine gamma‐lyase aggravates periodontal damage in traumatic occlusion mouse models. Journal of periodontal research, 55(5), 667-675.

Akahoshi, N., Handa, H., Takemoto, R., Kamata, S., Yoshida, M., Onaka, T., & Ishii, I. (2019). Preeclampsia-like features and partial lactation failure in mice lacking cystathionine γ-lyase—an animal model of cystathioninuria. International Journal of Molecular Sciences, 20(14), 3507.

Wang, Y. H., Huang, J. T., Chen, W. L., Wang, R. H., Kao, M. C., Pan, Y. R., ... & Wang, L. H. (2019). Dysregulation of cystathionine γ‐lyase promotes prostate cancer progression and metastasis. EMBO reports, 20(10), e45986.

Lechuga, T. J., Qi, Q. R., Kim, T., Magness, R. R., & Chen, D. B. (2019). E2β stimulates ovine uterine artery endothelial cell H2S production in vitro by estrogen receptor-dependent upregulation of cystathionine β-synthase and cystathionine γ-lyase expression. Biology of reproduction, 100(2), 514-522.

Szijártó, I. A., Markó, L., Filipovic, M. R., Miljkovic, J. L., Tabeling, C., Tsvetkov, D., ... & Gollasch, M. (2018). Cystathionine γ-Lyase–Produced hydrogen sulfide controls endothelial no bioavailability and blood pressure. Hypertension, 71(6), 1210-1217.

Gupta, V., Kapopara, P. R., Khan, A. A., Arige, V., Subramanian, L., Sonawane, P. J., ... & Mahapatra, N. R. (2017). Functional promoter polymorphisms direct the expression of cystathionine gamma-lyase gene in mouse models of essential hypertension. Journal of Molecular and Cellular Cardiology, 102, 61-73.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

CTH Matched Antibody Pair (309) (CAT#: APMAB-309LY)

CTH Matched Antibody Pair (310) (CAT#: APMAB-310LY)

CTH Matched Antibody Pair (311) (CAT#: APMAB-311LY)

CTH Matched Antibody Pair (312) (CAT#: APMAB-312LY)

CTH Matched Antibody Pair (313) (CAT#: APMAB-313LY)

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CTH Matched Antibody Pair (309) (CAT#: APMAB-309LY)

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CTH Matched Antibody Pair (312) (CAT#: APMAB-312LY)

Mouse Anti-CTH Recombinant Antibody (4E1-1B7) (CAT#: V2LY-0125-LY256)

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CTH Matched Antibody Pair (311) (CAT#: APMAB-311LY)

Mouse Anti-CTH Recombinant Antibody (CBLG1-379) (CAT#: CBMAB-G1487-LY)

Mouse Anti-CTH Recombinant Antibody (CBWJC-3329) (CAT#: V2LY-0125-LY261)

Isotype control

For research use only. Not intended for any clinical use.

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