Mouse Anti-DBF4B Recombinant Antibody (1A7) (V2LY-0125-LY1137)

Name: Mouse Anti-DBF4B Recombinant Antibody (1A7)
SKU: V2LY-0125-LY1137
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1A7

Application

ELISA, WB

Immunogen

DBF4B (201-299aa) partial recombinant protein.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

DBF4 homolog B (S. cerevisiae)

Entrez Gene ID

80174

UniProt ID

Q8NFT6

Function

Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S and M phases. The complex CDC7-DBF4B selectively phosphorylates MCM2 subunit at 'Ser-40' and then is involved in regulating the initiation of DNA replication during cell cycle.

Biological Process

Cell cycle Source: UniProtKB-KW
Positive regulation of cell population proliferation Source: UniProtKB
Positive regulation of G2/M transition of mitotic cell cycle Source: UniProtKB
Positive regulation of nuclear cell cycle DNA replication Source: UniProtKB
Regulation of cell cycle phase transition Source: GO_Central

Cellular Location

Nucleus. Predominantly found in soluble fraction but not in the chromatin-bound fraction.

PTM

Phosphorylated.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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