Mouse Anti-DIABLO Recombinant Antibody (D0868) (V2LY-0425-LY306)

Name: Mouse Anti-DIABLO Recombinant Antibody (D0868)
SKU: V2LY-0425-LY306
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

D0868

Application

IHC, WB, IP

Immunogen

Recombinant mature human Smac.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500
IP	1:10-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Ascites

Preservative

Sodium Azide

Concentration

Batch dependent

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Diablo homolog, mitochondrial

Entrez Gene ID

Human	56616
Mouse	66593
Rat	288753

UniProt ID

Human	Q9NR28
Mouse	Q9JIQ3
Rat	Q5RK17

Function

Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance.

Biological Process

Activation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
Activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c Source: GO_Central
Apoptotic process Source: ProtInc
Extrinsic apoptotic signaling pathway via death domain receptors Source: ProtInc
Intrinsic apoptotic signaling pathway Source: UniProtKB
Intrinsic apoptotic signaling pathway in response to oxidative stress Source: GO_Central
Neuron apoptotic process Source: GO_Central
Positive regulation of apoptotic process Source: UniProtKB

Cellular Location

Mitochondrion. Released into the cytosol when cells undergo apoptosis.

Involvement in disease

Deafness, autosomal dominant, 64 (DFNA64):
A form of non-syndromic sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.

PTM

Ubiquitinated by BIRC7/livin.

Zhao, X. Y., Wang, X. Y., Wei, Q. Y., Xu, Y. M., & Lau, A. T. (2020). Potency and selectivity of SMAC/DIABLO mimetics in solid tumor therapy. Cells, 9(4), 1012.

Lv, Z., Song, X., Xu, J., Jia, Z., Yang, B., Jia, Y., ... & Song, L. (2019). The modulation of Smac/DIABLO on mitochondrial apoptosis induced by LPS in Crassostrea gigas. Fish & shellfish immunology, 84, 587-598.

Campbell, G. R., & Spector, S. A. (2019). DIABLO/SMAC mimetics selectively kill HIV-1-infected resting memory CD4+ T cells: a potential role in a cure strategy for HIV-1 infection. Autophagy, 15(4), 744-746.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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