Mouse Anti-DYRK4 Recombinant Antibody (D2032) (V2LY-0425-LY1166)

Name: Mouse Anti-DYRK4 Recombinant Antibody (D2032)
SKU: V2LY-0425-LY1166
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

D2032

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 1-104 mapping at the N-terminus of human Dyrk4.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4

Entrez Gene ID

8798

UniProt ID

Q9NR20

Research Area

Possible non-essential role in spermiogenesis.

Biological Process

Peptidyl-serine phosphorylation Source: GO_Central
Peptidyl-threonine phosphorylation Source: GO_Central

Cellular Location

Isoform 1: Cytoplasm
Isoform 2: Cytoplasm; Nucleus.

PTM

Autophosphorylation on Tyr-264 in the activation loop is required for kinase activity.

Liu, C. C., Veeraraghavan, J., Tan, Y., Kim, J. A., Wang, X., Loo, S. K., ... & Wang, X. S. (2021). A Novel Neoplastic Fusion Transcript, RAD51AP1-DYRK4, Confers Sensitivity to the MEK Inhibitor Trametinib in Aggressive Breast CancersNeoplastic RAD51AP1-DYRK4 Fusion Transcript in Breast Cancer. Clinical Cancer Research, 27(3), 785-798.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Mouse Anti-DYRK4 Recombinant Antibody (CBT4752) (CAT#: V2LY-0625-LY1891)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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