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Mouse Anti-E2F1 (AA 1-89) Recombinant Antibody (KH95) (CBMAB-E0654-FY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
KH95
Antibody Isotype
IgG2a, κ
Application
GS, IF, IHC-P, IP, WB, IHC-Fr

Basic Information

Immunogen
GST fusion protein corresponding to full-length human E2F-1
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Concentration
1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 1-89

Target

Full Name
E2F Transcription Factor 1
Introduction
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain.
Entrez Gene ID
1869
UniProt ID
Q01094
Alternative Names
RBP3; E2F-1; RBAP1; RBBP3
Research Area
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters (PubMed:20176812).

Positively regulates transcription of RRP1B (PubMed:20040599).
Biological Process
Anoikis Source: Ensembl
Cellular response to fatty acid Source: Ensembl
Cellular response to hypoxia Source: Ensembl
Cellular response to nerve growth factor stimulus Source: Ensembl
Cellular response to xenobiotic stimulus Source: Ensembl
DNA damage checkpoint signaling Source: UniProtKB
Forebrain development Source: Ensembl
G1/S transition of mitotic cell cycle Source: GO_Central
Intrinsic apoptotic signaling pathway by p53 class mediator Source: Ensembl
Intrinsic apoptotic signaling pathway in response to DNA damage Source: UniProtKB
Lens fiber cell apoptotic process Source: Ensembl
mRNA stabilization Source: BHF-UCL
Negative regulation of DNA binding Source: UniProtKB
Negative regulation of fat cell differentiation Source: UniProtKB
Negative regulation of fat cell proliferation Source: UniProtKB
Negative regulation of transcription, DNA-templated Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: BHF-UCL
Negative regulation of transcription involved in G1/S transition of mitotic cell cycle Source: BHF-UCL
Positive regulation of apoptotic process Source: UniProtKB
Positive regulation of fibroblast proliferation Source: BHF-UCL
Positive regulation of gene expression Source: BHF-UCL
Positive regulation of glial cell proliferation Source: Ensembl
Positive regulation of transcription, DNA-templated Source: BHF-UCL
Positive regulation of transcription by RNA polymerase II Source: UniProtKB
Regulation of G1/S transition of mitotic cell cycle Source: BHF-UCL
Regulation of transcription, DNA-templated Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central
Spermatogenesis Source: Ensembl
Transcription, DNA-templated Source: UniProtKB
Cellular Location
Nucleus
PTM
Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. Phosphorylation at Ser-364 by CHEK2 stabilizes E2F1 upon DNA damage and regulates its effect on transcription and apoptosis.
Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein RB1. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B.
More Infomation

Ma, X., Li, Y., & Zhao, B. (2022). Ribosomal protein L5 (RPL5)/E2F transcription factor 1 (E2F1) signaling suppresses breast cancer progression via regulating endoplasmic reticulum stress and autophagy. Bioengineered, 13(4), 8076-8086.

Zhou, Z. Y., Yang, J. Y., Shao, C. Z., Luo, F., & Du, W. (2022). Positive regulation of ataxia-telangiectasia-mutated protein (ATM) by E2F transcription Factor 1 (E2F-1) in cisplatin-resistant nasopharyngeal carcinoma cells. World Journal of Surgical Oncology, 20(1), 1-11.

Xie, H., Lv, S., Wang, Z., & Yuan, X. (2021). E2F transcription factor 1 elevates cyclin D1 expression by suppressing transcription of microRNA‐107 to augment progression of glioma. Brain and Behavior, 11(12), e2399.

Ye, W. Y., Lu, H. P., Li, J. D., Chen, G., He, R. Q., Wu, H. Y., ... & Dang, Y. W. (2021). Clinical Implication of E2F Transcription Factor 1 in Hepatocellular Carcinoma Tissues. Cancer Biotherapy & Radiopharmaceuticals.

Liao, R., Xie, B., Cui, J., Qi, Z., Xue, S., & Wang, Y. (2021). E2F transcription factor 1 (E2F1) promotes the transforming growth factor TGF-β1 induced human cardiac fibroblasts differentiation through promoting the transcription of CCNE2 gene. Bioengineered, 12(1), 6869-6877.

Fang, Z., Lin, M., Li, C., Liu, H., & Gong, C. (2020). A comprehensive review of the roles of E2F1 in colon cancer. American journal of cancer research, 10(3), 757.

Zheng, H., Wang, J. J., Yang, X. R., & Yu, Y. L. (2020). Upregulation of miR-34c after silencing E2F transcription factor 1 inhibits paclitaxel combined with cisplatin resistance in gastric cancer cells. World journal of gastroenterology, 26(5), 499.

Morrell, B. C., Perego, M. C., Maylem, E. R. S., Zhang, L., Schütz, L. F., & Spicer, L. J. (2020). Regulation of the transcription factor E2F1 mRNA in ovarian granulosa cells of cattle. Journal of Animal Science, 98(1), skz376.

Chun, J. N., Cho, M., Park, S., So, I., & Jeon, J. H. (2020). The conflicting role of E2F1 in prostate cancer: A matter of cell context or interpretational flexibility?. Biochimica et Biophysica Acta (BBA)-Reviews on Cancer, 1873(1), 188336.

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For research use only. Not intended for any clinical use.

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