Mouse Anti-EPHX1 Recombinant Antibody (CBFYE-0078) (CBMAB-E0169-FY)

Name: Mouse Anti-EPHX1 Recombinant Antibody (CBFYE-0078)
SKU: CBMAB-E0169-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-0078

Application

WB, ELISA

Immunogen

Recombinant human EPHX1 (21-455aa) purified from E. coli.

Host Species

Mouse

Specificity

Human, Mouse

Antibody Isotype

IgG2a

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Epitope

AA 21-455

More Infomation

Target

Full Name

epoxide hydrolase 1, microsomal (xenobiotic)

Introduction

Epoxide hydrolase is a critical biotransformation enzyme that converts epoxides from the degradation of aromatic compounds to trans-dihydrodiols which can be conjugated and excreted from the body. Epoxide hydrolase functions in both the activation and detoxification of epoxides. Mutations in this gene cause preeclampsia, epoxide hydrolase deficiency or increased epoxide hydrolase activity. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

Entrez Gene ID

Human	2052
Mouse	13849

UniProt ID

Human	P07099
Mouse	Q9D379

Alternative Names

Epoxide Hydrolase 1; Epoxide Hydrolase 1, Microsomal (Xenobiotic); Epoxide Hydratase; EPHX; EPOX; MEH

Research Area

Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water (By similarity).

Plays a role in the metabolism of endogenous lipids such as epoxide-containing fatty acids (PubMed:22798687).

Metabolizes the abundant endocannabinoid 2-arachidonoylglycerol (2-AG) to free arachidonic acid (AA) and glycerol (PubMed:24958911).

Biological Process

Arachidonic acid metabolic process Source: UniProtKB
Aromatic compound catabolic process Source: UniProtKB-KW
Epoxide metabolic process Source: UniProtKB
Response to organic cyclic compound Source: Ensembl
Response to toxic substance Source: UniProtKB-KW
Xenobiotic metabolic process Source: Reactome

Cellular Location

Microsome membrane; Endoplasmic reticulum membrane

Involvement in disease

In some populations, the high activity haplotype tyr113/his139 is overrepresented among women suffering from pregnancy-induced hypertension (pre-eclampsia) when compared with healthy controls.
Familial hypercholanemia (FHCA):
A disorder characterized by elevated serum bile acid concentrations, itching, and fat malabsorption.

Topology

Helical: 1-21
Cytoplasmic: 22-455

Hasibuan, R. H., Soeroso, N. N., Tarigan, S. P., Siregar, Y., Mutiara, E., & Aktalina, L. (2022). The Relationship of Genetic Polymorphism Micromal Epoxide Hydrolase (EPHX1) His139Arg and Lung Cancer. Jurnal Respirologi Indonesia, 42(2), 86-89.

Hu, T., Zeng, X., Tian, T., & Liu, J. (2021). Association of EPHX1 polymorphisms with plasma concentration of carbamazepine in epileptic patients: Systematic review and meta-analysis. Journal of Clinical Neuroscience, 91, 159-171.

Gautheron, J., Morisseau, C., Chung, W. K., Zammouri, J., Auclair, M., Baujat, G., ... & Jeru, I. (2021). EPHX1 mutations cause a lipoatrophic diabetes syndrome due to impaired epoxide hydrolysis and increased cellular senescence. Elife, 10, e68445.

Demirdöğen, B. C., Miçooğulları, Y., Özçelik, A. T., & Adalı, O. (2020). Missense genetic polymorphisms of microsomal (EPHX1) and soluble epoxide hydrolase (EPHX2) and their relation to the risk of large artery atherosclerotic ischemic stroke in a Turkish population. Neuropsychiatric disease and treatment, 16, 3251.

Long, X., Li, Q., Zhi, L. J., Li, J. M., & Wang, Z. Y. (2020). LINC00205 modulates the expression of EPHX1 through the inhibition of miR‐184 in hepatocellular carcinoma as a ceRNA. Journal of Cellular Physiology, 235(3), 3013-3021.

Wang, Q., Xu, X., Cong, X., Zeng, Z., Xu, L., & Huo, X. (2019). Interactions between polycyclic aromatic hydrocarbons and epoxide hydrolase 1 play roles in asthma. Environmental geochemistry and health, 41(1), 191-210.

Jin, Y. (2019). Association between EPHX1 polymorphism rs1051740 and the risk of ovarian cancer: a meta-analysis. Artificial Cells, Nanomedicine, and Biotechnology, 47(1), 2338-2342.

Edin, M. L., Hamedani, B. G., Gruzdev, A., Graves, J. P., Lih, F. B., Arbes, S. J., ... & Zeldin, D. C. (2018). Epoxide hydrolase 1 (EPHX1) hydrolyzes epoxyeicosanoids and impairs cardiac recovery after ischemia. Journal of biological chemistry, 293(9), 3281-3292.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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