Mouse Anti-EPHX2 Recombinant Antibody (CBFYE-1041) (V2LY-0825-LY517)

Name: Mouse Anti-EPHX2 Recombinant Antibody (CBFYE-1041)
SKU: V2LY-0825-LY517
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-1041

Application

IF, ELISA, IHC-P, WB, IP

Immunogen

Amino acids 340-554 mapping at the C-terminus of human sEH.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Epoxide Hydrolase 2

Entrez Gene ID

2053

UniProt ID

P34913

Research Area

Bifunctional enzyme (PubMed:12574510).

The C-terminal domain has epoxide hydrolase activity and acts on epoxides (alkene oxides, oxiranes) and arene oxides (PubMed:12869654, PubMed:12574510, PubMed:22798687).

Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides (By similarity).

Also determines steady-state levels of physiological mediators (PubMed:12869654, PubMed:12574510, PubMed:22798687, PubMed:21217101).

Bifunctional enzyme (PubMed:12574510).

The N-terminal domain has lipid phosphatase activity, with the highest activity towards threo-9,10-phosphonooxy-hydroxy-octadecanoic acid, followed by erythro-9,10-phosphonooxy-hydroxy-octadecanoic acid, 12-phosphonooxy-octadec-9Z-enoic acid and 12-phosphonooxy-octadec-9E-enoic acid (PubMed:12574510).

Has phosphatase activity toward lyso-glycerophospholipids with also some lower activity toward lysolipids of sphingolipid and isoprenoid phosphates (PubMed:22217705, PubMed:22387545).

Biological Process

Cholesterol homeostasis Source: UniProtKB
Dephosphorylation Source: UniProtKB
Epoxide metabolic process Source: UniProtKB
Phospholipid dephosphorylation Source: UniProtKB
Positive regulation of gene expression Source: UniProtKB
Regulation of cholesterol metabolic process Source: UniProtKB
Response to toxic substance Source: UniProtKB-KW
Stilbene catabolic process Source: UniProtKB

Cellular Location

Cytoplasm; Peroxisome

PTM

The N-terminus is blocked.
The covalent modification of cysteine by 15-deoxy-Delta12,14-prostaglandin-J2 is autocatalytic and reversible. It may occur as an alternative to other cysteine modifications, such as S-nitrosylation and S-palmitoylation (Probable).

Roumeliotis, S., Roumeliotis, A., Stamou, A., Panagoutsos, S., Manolopoulos, V. G., Tsetsos, F., ... & Liakopoulos, V. (2021). Association of rs11780592 polymorphism in the human soluble epoxide hydrolase gene (EPHX2) with oxidized LDL and mortality in patients with diabetic chronic kidney disease. Oxidative medicine and cellular longevity, 2021.

Li, X., & Wu, X. (2021). Soluble epoxide hydrolase (Ephx2) silencing attenuates the hydrogen peroxide-induced oxidative damage in IEC-6 cells. Archives of Medical Science: AMS, 17(4), 1075.

Khadir, A., Kavalakatt, S., Madhu, D., Cherian, P., Al-Mulla, F., Abubaker, J., & Tiss, A. (2020). Soluble epoxide hydrolase 2 expression is elevated in obese humans and decreased by physical activity. International journal of molecular sciences, 21(6), 2056.

Demirdöğen, B. C., Miçooğulları, Y., Özçelik, A. T., & Adalı, O. (2020). Missense genetic polymorphisms of microsomal (EPHX1) and soluble epoxide hydrolase (EPHX2) and their relation to the risk of large artery atherosclerotic ischemic stroke in a Turkish population. Neuropsychiatric disease and treatment, 16, 3251.

Ma, L., Zhao, H., Yu, M., Wen, Y., Zhao, T., Yan, M., ... & Niu, W. (2020). Association of epoxide hydrolase 2 gene Arg287Gln with the risk for primary hypertension in Chinese. International journal of hypertension, 2020.

Sun, H., Lee, P., Yan, C., Gao, N., Wang, J., Fan, X., & Yu, F. S. (2018). Inhibition of soluble epoxide hydrolase 2 ameliorates diabetic keratopathy and impaired wound healing in mouse corneas. Diabetes, 67(6), 1162-1172.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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