Mouse Anti-G3BP1 Recombinant Antibody (2F3) (CBMAB-A3259-LY)

Name: Mouse Anti-G3BP1 Recombinant Antibody (2F3)
SKU: CBMAB-A3259-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

2F3

Application

WB, ELISA

Immunogen

G3BP (AAH06997, 214 a.a. ~ 303 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

G3BP1

Introduction

This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq]

Entrez Gene ID

10146

UniProt ID

Q13283

Alternative Names

G3BP; HDH-VIII; MGC111040

Function

ATP- and magnesium-dependent helicase that plays an essential role in innate immunity (PubMed:30510222).

Participates in the DNA-triggered cGAS/STING pathway by promoting the DNA binding and activation of CGAS. Enhances also DDX58-induced type I interferon production probably by helping DDX58 at sensing pathogenic RNA (PubMed:30804210).

In addition, plays an essential role in stress granule formation (PubMed:12642610, PubMed:20180778, PubMed:23279204).

Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends (PubMed:9889278).

Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency (PubMed:9889278).

Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA (PubMed:9889278).

Phosphorylation-dependent sequence-specific endoribonuclease in vitro (PubMed:11604510).

Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR (PubMed:11604510).

Biological Process

Defense response to virus Source: UniProtKB
Innate immune response Source: UniProtKB-KW
Negative regulation of canonical Wnt signaling pathway Source: Ensembl
Positive regulation of stress granule assembly Source: UniProtKB
Positive regulation of type I interferon production Source: UniProtKB
Ras protein signal transduction Source: ProtInc
Stress granule assembly Source: UniProtKB

Cellular Location

Cytosol; Nucleus; Perikaryon; Stress granule. Cytoplasmic in proliferating cells (PubMed:11604510). Cytosolic and partially nuclear in resting cells (PubMed:11604510). Recruited to stress granules in response to arsenite treatment (PubMed:12642610, PubMed:20180778). The unphosphorylated form is recruited to stress granules (PubMed:12642610). HRAS signaling contributes to this process by regulating G3BP dephosphorylation (PubMed:12642610).

PTM

(Microbial infection) Cleaved by human enterovirus 71; this cleavage induces the disassembly of cytoplasmic stress granules (PubMed:30006004). Cleaved by Foot-and-mouth disease virus; this cleavage suppresses the formation of cytoplasmic stress granules (PubMed:30404792).
Phosphorylated exclusively on serine residues. Hyperphosphorylated in quiescent fibroblasts. Hypophosphorylation leads to a decrease in endoribonuclease activity (By similarity). RASA1-dependent phosphorylation of Ser-149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. Ser-149 phosphorylation induces partial nuclear localization.
Arg-435 is dimethylated, probably to asymmetric dimethylarginine.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Anti-G3BP1 Recombinant Antibody (2F3) (CAT#: CBMAB-G1547-LY)

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Mouse Anti-G3BP1 Recombinant Antibody (2F3) (CAT#: CBMAB-G1547-LY)

Isotype control

For research use only. Not intended for any clinical use.

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