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Mouse Anti-GPC3 (AA 121-220) Recombinant Antibody (CBFYH-0434) (CBMAB-H1309-FY)

This product is mouse antibody that recognizes GPC3. The antibody CBFYH-0434 can be used for immunoassay techniques such as: ELISA, WB.
See all GPC3 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYH-0434
Antibody Isotype
IgM
Application
ELISA, WB

Basic Information

Immunogen
GPC3 (121 a.a. ~ 220 a.a) partial recombinant protein with GST tag
Specificity
Human
Antibody Isotype
IgM
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Supernatant
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 121-220

Target

Full Name
Glypican 3
Introduction
Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants.
Entrez Gene ID
2719
UniProt ID
P51654
Alternative Names
Glypican 3; Intestinal Protein OCI-5; Glypican Proteoglycan 3; GTR2-2; MXR7; Heparan Sulphate Proteoglycan; Secreted Glypican-3; Glypican-3
Function
Cell surface proteoglycan that bears heparan sulfate (PubMed:14610063).

Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins (By similarity).

Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation (By similarity).

Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands (PubMed:16227623, PubMed:24496449).

Positively regulates the non-canonical Wnt signaling pathway (By similarity).

Binds to CD81 which decreases the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression (By similarity).

Inhibits the dipeptidyl peptidase activity of DPP4 (PubMed:17549790).

Plays a role in limb patterning and skeletal development by controlling the cellular response to BMP4 (By similarity).

Modulates the effects of growth factors BMP2, BMP7 and FGF7 on renal branching morphogenesis (By similarity).

Required for coronary vascular development (By similarity).

Plays a role in regulating cell movements during gastrulation (By similarity).
Biological Process
Anatomical structure morphogenesis Source: ProtInc
Anterior/posterior axis specification Source: Ensembl
Body morphogenesis Source: Ensembl
Bone mineralization Source: Ensembl
Branching involved in ureteric bud morphogenesis Source: Ensembl
Cell migration Source: GO_Central
Cell migration involved in gastrulation Source: UniProtKB
Cell proliferation involved in kidney development Source: UniProtKB
Cell proliferation involved in metanephros development Source: Ensembl
Coronary vasculature development Source: UniProtKB
Embryonic hindlimb morphogenesis Source: Ensembl
Lung development Source: Ensembl
Mesenchymal cell proliferation involved in ureteric bud development Source: UniProtKB
Mesonephric duct morphogenesis Source: UniProtKB
Negative regulation of canonical Wnt signaling pathway Source: Ensembl
Negative regulation of epithelial cell proliferation Source: Ensembl
Negative regulation of growth Source: Ensembl
Negative regulation of peptidase activity Source: UniProtKB-KW
Negative regulation of smoothened signaling pathway Source: UniProtKB
Osteoclast differentiation Source: Ensembl
Positive regulation of BMP signaling pathway Source: Ensembl
Positive regulation of canonical Wnt signaling pathway Source: UniProtKB
Positive regulation of endocytosis Source: UniProtKB
Positive regulation of glucose import Source: Ensembl
Positive regulation of protein catabolic process Source: UniProtKB
Positive regulation of smoothened signaling pathway Source: Ensembl
Positive regulation of Wnt signaling pathway, planar cell polarity pathway Source: Ensembl
Regulation of canonical Wnt signaling pathway Source: UniProtKB
Regulation of non-canonical Wnt signaling pathway Source: UniProtKB
Regulation of protein localization to membrane Source: GO_Central
Response to bacterium Source: Ensembl
Cellular Location
Cell membrane
Involvement in disease
Simpson-Golabi-Behmel syndrome 1 (SGBS1):
A condition characterized by pre- and postnatal overgrowth (gigantism), facial dysmorphism and a variety of inconstant visceral and skeletal malformations. Characteristic dysmorphic features include macrocephaly with coarse, distinctive facies with a large protruding jaw, broad nasal bridge and cleft palate. Cardiac defects are frequent.
PTM
O-glycosylated; contains heparan sulfate.
Cleaved intracellularly by a furin-like convertase to generate 2 subunits, alpha and beta, which remain associated through disulfide bonds and are associated with the cell surface via the GPI-anchor (PubMed:14610063). This processing is essential for its role in inhibition of hedgehog signaling (PubMed:25653284). A second proteolytic event may result in cleavage of the protein on the cell surface, separating it from the GPI-anchor and leading to its shedding from the cell surface (PubMed:14610063).

Makkouk, A., Yang, X. C., Barca, T., Lucas, A., Turkoz, M., Wong, J. T., ... & Herrman, M. (2021). Off-the-shelf Vδ1 gamma delta T cells engineered with glypican-3 (GPC-3)-specific chimeric antigen receptor (CAR) and soluble IL-15 display robust antitumor efficacy against hepatocellular carcinoma. Journal for immunotherapy of cancer, 9(12).

Guereño, M., Pastore, M. D., Lugones, A. C., Cercato, M., Todaro, L., Urtreger, A., & Peters, M. G. (2020). Glypican-3 (GPC3) inhibits metastasis development promoting dormancy in breast cancer cells by p38 MAPK pathway activation. European Journal of Cell Biology, 99(6), 151096.

Liu, S., Wang, M., Zheng, C., Zhong, Q., Shi, Y., & Han, X. (2020). Diagnostic value of serum glypican-3 alone and in combination with AFP as an aid in the diagnosis of liver cancer. Clinical biochemistry, 79, 54-60.

Batra, S. A., Rathi, P., Guo, L., Courtney, A. N., Fleurence, J., Balzeau, J., ... & Heczey, A. (2020). Glypican-3–Specific CAR T Cells Coexpressing IL15 and IL21 Have Superior Expansion and Antitumor Activity against Hepatocellular CarcinomaIL15 and IL21 Armored GPC3-CAR T Cells against Liver Cancer. Cancer immunology research, 8(3), 309-320.

Fu, Y., Urban, D. J., Nani, R. R., Zhang, Y. F., Li, N., Fu, H., ... & Ho, M. (2019). Glypican‐3‐specific antibody drug conjugates targeting hepatocellular carcinoma. Hepatology, 70(2), 563-576.

Nishida, T., & Kataoka, H. (2019). Glypican 3-targeted therapy in hepatocellular carcinoma. Cancers, 11(9), 1339.

Shimizu, Y., Suzuki, T., Yoshikawa, T., Endo, I., & Nakatsura, T. (2019). Next-generation cancer immunotherapy targeting glypican-3. Frontiers in oncology, 9, 248.

Xu, D., Su, C., Sun, L., Gao, Y., & Li, Y. (2019). Performance of serum glypican 3 in diagnosis of hepatocellular carcinoma: a meta-analysis. Annals of Hepatology, 18(1), 58-67.

Liu, H., Yang, C., Lu, W., & Zeng, Y. (2018). Prognostic significance of glypican-3 expression in hepatocellular carcinoma: A meta-analysis. Medicine, 97(4).

Zhou, F., Shang, W., Yu, X., & Tian, J. (2018). Glypican‐3: a promising biomarker for hepatocellular carcinoma diagnosis and treatment. Medicinal research reviews, 38(2), 741-767.

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For research use only. Not intended for any clinical use.

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