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Mouse Anti-GPR161 Recombinant Antibody (CBLG1-1632) (CBMAB-G4636-LY)

This product is antibody recognizes GPR161. The antibody CBLG1-1632 immunoassay techniques such as: ELISA, WB.
See all GPR161 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBLG1-1632
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
G protein-coupled receptor 161
Introduction
This gene belongs to the G-protein coupled receptor 1 family. G protein-coupled receptors contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins. The protein encoded by this gene is most closely related to biogenic amine receptors. [provided by RefSeq, Jul 2008]
Entrez Gene ID
23432
UniProt ID
Q8N6U8
Alternative Names
G Protein-Coupled Receptor 161; G-Protein Coupled Receptor RE2; G-Protein Coupled Receptor 161; RE2;
Function
Key negative regulator of Shh signaling, which promotes the processing of GLI3 into GLI3R during neural tube development. Recruited by TULP3 and the IFT-A complex to primary cilia and acts as a regulator of the PKA-dependent basal repression machinery in Shh signaling by increasing cAMP levels, leading to promote the PKA-dependent processing of GLI3 into GLI3R and repress the Shh signaling. In presence of SHH, it is removed from primary cilia and is internalized into recycling endosomes, preventing its activity and allowing activation of the Shh signaling. Its ligand is unknown (By similarity).
Biological Process
Adenylate cyclase-activating G protein-coupled receptor signaling pathway Source: UniProtKB
G protein-coupled receptor signaling pathway Source: GO_Central
Negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterning Source: UniProtKB
Cellular Location
Cilium membrane; Cell membrane. Mainly localizes to primary cilium in a TULP3 and IFT-A complex-dependent manner. In presence of SHH, it is removed from primary cilia and is internalized into recycling endosomes and is apparently not degraded (By similarity).
Topology
Extracellular: 1-30
Helical: 31-51
Cytoplasmic: 52-64
Helical: 65-85
Extracellular: 86-101
Helical: 102-123
Cytoplasmic: 124-143
Helical: 144-164
Extracellular: 165-190
Helical: 191-211
Cytoplasmic: 212-269
Helical: 270-290
Extracellular: 291-306
Helical: 307-327
Cytoplasmic: 328-529

Patel, K., & Smith, N. J. (2023). Primary cilia, A‐kinase anchoring proteins and constitutive activity at the orphan G protein‐coupled receptor GPR161: A tale about a tail. British Journal of Pharmacology.

Chou, C. H., Wang, H. K., Lin, Y. C., Tsai, D. H., Lu, M. T., Ho, C. T., ... & Way, T. D. (2021). Bisdemethoxycurcumin Promotes Apoptosis and Inhibits the Epithelial–Mesenchymal Transition through the Inhibition of the G-Protein-Coupled Receptor 161/Mammalian Target of Rapamycin Signaling Pathway in Triple Negative Breast Cancer Cells. Journal of agricultural and food chemistry, 69(48), 14557-14567.

Hannah-Shmouni, F., & Stratakis, C. A. (2021). GPR101, an orphan G-protein coupled receptor, with roles in growth, puberty, and possibly appetite regulation. In Gigantism and Acromegaly (pp. 79-88). Academic Press.

Begemann, M., Waszak, S. M., Robinson, G. W., Jäger, N., Sharma, T., Knopp, C., ... & Kurth, I. (2020). Germline GPR161 mutations predispose to pediatric medulloblastoma. Journal of Clinical Oncology, 38(1), 43.

Wang, M., Li, P., Wang, H., Dong, L., Wu, C., & Zhao, Z. (2020). Identification and spatiotemporal expression of gpr161 genes in zebrafish. Gene, 730, 144303.

Tschaikner, P., Enzler, F., Torres-Quesada, O., Aanstad, P., & Stefan, E. (2020). Hedgehog and Gpr161: regulating cAMP signaling in the primary cilium. Cells, 9(1), 118.

Trivellin, G., Hernández-Ramírez, L. C., Swan, J., & Stratakis, C. A. (2018). An orphan G-protein-coupled receptor causes human gigantism and/or acromegaly: Molecular biology and clinical correlations. Best Practice & Research Clinical Endocrinology & Metabolism, 32(2), 125-140.

Pusapati, G. V., Kong, J. H., Patel, B. B., Gouti, M., Sagner, A., Sircar, R., ... & Rohatgi, R. (2018). G protein–coupled receptors control the sensitivity of cells to the morphogen Sonic Hedgehog. Science Signaling, 11(516), eaao5749.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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