Mouse Anti-GPR161 Recombinant Antibody (CBLG1-200) (CBMAB-G0708-LY)

Name: Mouse Anti-GPR161 Recombinant Antibody (CBLG1-200)
SKU: CBMAB-G0708-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBLG1-200

Application

ELISA, WB

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

G protein-coupled receptor 161

Introduction

This gene belongs to the G-protein coupled receptor 1 family. G protein-coupled receptors contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins. The protein encoded by this gene is most closely related to biogenic amine receptors. [provided by RefSeq, Jul 2008]

Entrez Gene ID

23432

UniProt ID

Q8N6U8

Alternative Names

G Protein-Coupled Receptor 161; G-Protein Coupled Receptor RE2; G-Protein Coupled Receptor 161; RE2;

Function

Key negative regulator of Shh signaling, which promotes the processing of GLI3 into GLI3R during neural tube development. Recruited by TULP3 and the IFT-A complex to primary cilia and acts as a regulator of the PKA-dependent basal repression machinery in Shh signaling by increasing cAMP levels, leading to promote the PKA-dependent processing of GLI3 into GLI3R and repress the Shh signaling. In presence of SHH, it is removed from primary cilia and is internalized into recycling endosomes, preventing its activity and allowing activation of the Shh signaling. Its ligand is unknown (By similarity).

Biological Process

Adenylate cyclase-activating G protein-coupled receptor signaling pathway Source: UniProtKB
G protein-coupled receptor signaling pathway Source: GO_Central
Negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterning Source: UniProtKB

Cellular Location

Cilium membrane; Cell membrane. Mainly localizes to primary cilium in a TULP3 and IFT-A complex-dependent manner. In presence of SHH, it is removed from primary cilia and is internalized into recycling endosomes and is apparently not degraded (By similarity).

Topology

Extracellular: 1-30
Helical: 31-51
Cytoplasmic: 52-64
Helical: 65-85
Extracellular: 86-101
Helical: 102-123
Cytoplasmic: 124-143
Helical: 144-164
Extracellular: 165-190
Helical: 191-211
Cytoplasmic: 212-269
Helical: 270-290
Extracellular: 291-306
Helical: 307-327
Cytoplasmic: 328-529

Patel, K., & Smith, N. J. (2023). Primary cilia, A‐kinase anchoring proteins and constitutive activity at the orphan G protein‐coupled receptor GPR161: A tale about a tail. British Journal of Pharmacology.

Chou, C. H., Wang, H. K., Lin, Y. C., Tsai, D. H., Lu, M. T., Ho, C. T., ... & Way, T. D. (2021). Bisdemethoxycurcumin Promotes Apoptosis and Inhibits the Epithelial–Mesenchymal Transition through the Inhibition of the G-Protein-Coupled Receptor 161/Mammalian Target of Rapamycin Signaling Pathway in Triple Negative Breast Cancer Cells. Journal of agricultural and food chemistry, 69(48), 14557-14567.

Hannah-Shmouni, F., & Stratakis, C. A. (2021). GPR101, an orphan G-protein coupled receptor, with roles in growth, puberty, and possibly appetite regulation. In Gigantism and Acromegaly (pp. 79-88). Academic Press.

Begemann, M., Waszak, S. M., Robinson, G. W., Jäger, N., Sharma, T., Knopp, C., ... & Kurth, I. (2020). Germline GPR161 mutations predispose to pediatric medulloblastoma. Journal of Clinical Oncology, 38(1), 43.

Wang, M., Li, P., Wang, H., Dong, L., Wu, C., & Zhao, Z. (2020). Identification and spatiotemporal expression of gpr161 genes in zebrafish. Gene, 730, 144303.

Tschaikner, P., Enzler, F., Torres-Quesada, O., Aanstad, P., & Stefan, E. (2020). Hedgehog and Gpr161: regulating cAMP signaling in the primary cilium. Cells, 9(1), 118.

Trivellin, G., Hernández-Ramírez, L. C., Swan, J., & Stratakis, C. A. (2018). An orphan G-protein-coupled receptor causes human gigantism and/or acromegaly: Molecular biology and clinical correlations. Best Practice & Research Clinical Endocrinology & Metabolism, 32(2), 125-140.

Pusapati, G. V., Kong, J. H., Patel, B. B., Gouti, M., Sagner, A., Sircar, R., ... & Rohatgi, R. (2018). G protein–coupled receptors control the sensitivity of cells to the morphogen Sonic Hedgehog. Science Signaling, 11(516), eaao5749.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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