Rabbit Anti-GPRC5B Recombinant Antibody (EG1413) (CBMAB-EN1692-LY)

Name: Rabbit Anti-GPRC5B Recombinant Antibody (EG1413)
SKU: CBMAB-EN1692-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

EG1413

Application

WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000

Immunogen

The antibody was produced against synthesized peptide derived from internal of human GPRC5B.

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

G Protein-Coupled Receptor Class C Group 5 Member B

Introduction

This gene encodes a member of the type 3 G protein-coupled receptor family. Members of this superfamily are characterized by a signature 7-transmembrane domain motif. The encoded protein may modulate insulin secretion and increased protein expression is associated with type 2 diabetes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2015]

Entrez Gene ID

51704

UniProt ID

Q9NZH0

Alternative Names

G Protein-Coupled Receptor Class C Group 5 Member B; G Protein-Coupled Receptor, Family C, Group 1, Member B; Retinoic Acid-Induced Gene 2 Protein; RAIG-2; RAIG2; G Protein-Coupled Receptor, Family C, Group 5, Member B;

Function

Unknown. This retinoic acid-inducible G-protein coupled receptor provide evidence for a possible interaction between retinoid and G-protein signaling pathways.

Biological Process

Positive regulation of canonical Wnt signaling pathway Source: CAFA
Positive regulation of I-kappaB kinase/NF-kappaB signaling Source: CAFA
Positive regulation of inflammatory response Source: CAFA
Positive regulation of macrophage cytokine production Source: CAFA
Positive regulation of neuron differentiation Source: CAFA
Positive regulation of protein tyrosine kinase activity Source: CAFA

Cellular Location

Cell membrane; Cytoplasmic vesicle membrane. Localized in the plasma membrane and perinuclear vesicles.

Topology

Extracellular: 29-56
Helical: 57-77
Cytoplasmic: 78-94
Helical: 95-115
Extracellular: 116-126
Helical: 127-147
Cytoplasmic: 148-162
Helical: 163-183
Extracellular: 184-199
Helical: 200-220
Cytoplasmic: 221-234
Helical: 235-255
Extracellular: 256-271
Helical: 272-292
Cytoplasmic: 293-403

Fu, W., Franchini, L., & Orlandi, C. (2022). Comprehensive Spatial Profile of the Orphan G Protein Coupled Receptor GPRC5B Expression in Mouse Brain. Frontiers in Neuroscience, 16, 891544.

Xu, W., Nelson-Maney, N. P., Bálint, L., Kwon, H. B., Davis, R. B., Dy, D. C., ... & Caron, K. M. (2022). Orphan G-Protein Coupled Receptor GPRC5B Is Critical for Lymphatic Development. International Journal of Molecular Sciences, 23(10), 5712.

Alonso-Gardón, M., Elorza-Vidal, X., Castellanos, A., La Sala, G., Armand-Ugon, M., Gilbert, A., ... & Estévez, R. (2021). Identification of the GlialCAM interactome: the G protein-coupled receptors GPRC5B and GPR37L1 modulate megalencephalic leukoencephalopathy proteins. Human Molecular Genetics, 30(17), 1649-1665.

Hirabayashi, Y., & Kim, Y. J. (2020). Roles of GPRC5 family proteins: focusing on GPRC5B and lipid-mediated signalling. The journal of biochemistry, 167(6), 541-547.

Carvalho, J., Chennupati, R., Li, R., Günther, S., Kaur, H., Zhao, W., ... & Wettschureck, N. (2020). Orphan G protein–coupled receptor GPRC5B controls smooth muscle contractility and differentiation by inhibiting prostacyclin receptor signaling. Circulation, 141(14), 1168-1183.

Zambrano, S., Möller-Hackbarth, K., Li, X., Rodriguez, P. Q., Charrin, E., Schwarz, A., ... & Patrakka, J. (2019). GPRC5B modulates inflammatory response in glomerular diseases via NF-κB pathway. Journal of the American Society of Nephrology, 30(9), 1573-1586.

von Samson-Himmelstjerna, F. A., Freundt, G., Nitz, J. T., Stelter, F., Luedde, M., Wieland, T., ... & Hippe, H. J. (2019). The orphan receptor GPRC5B modulates inflammatory and fibrotic pathways in cardiac fibroblasts and mice hearts. Biochemical and biophysical research communications, 514(4), 1198-1203.

Kim, Y. J., Greimel, P., & Hirabayashi, Y. (2018). GPRC5B-mediated sphingomyelin synthase 2 phosphorylation plays a critical role in insulin resistance. IScience, 8, 250-266.

Sano, T., Kohyama-Koganeya, A., Kinoshita, M. O., Tatsukawa, T., Shimizu, C., Oshima, E., ... & Hirabayashi, Y. (2018). Loss of GPRC5B impairs synapse formation of Purkinje cells with cerebellar nuclear neurons and disrupts cerebellar synaptic plasticity and motor learning. Neuroscience research, 136, 33-47.

Atanes, P., Ruz-Maldonado, I., Hawkes, R., Liu, B., Persaud, S. J., & Amisten, S. (2018). Identifying signalling pathways regulated by GPRC5B in β-Cells by CRISPR-Cas9-mediated genome editing. Cellular Physiology and Biochemistry, 45(2), 656-666.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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