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Rabbit Anti-GPRC5B Recombinant Antibody (BA0082) (CBMAB-0353CQ)

This product is a rabbit antibody that recognizes GPRC5B. The antibody BA0082 can be used for immunoassay techniques such as: WB.
See all GPRC5B antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
BA0082
Antibody Isotype
IgG
Application
WB

Basic Information

Immunogen
Human GPCR GPRC5B aa 1-100
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
G Protein-Coupled Receptor Class C Group 5 Member B
Introduction
G protein-coupled receptors (GPCRs) share a common structure of 7 transmembrane domains, an extracellular N-terminal domain, an intracellular C-terminal domain, and several conserved residues. Family C of the GPCR superfamily, which includes GPRC5B, consists of metabotropic glutamate receptor-like proteins. This gene encodes a member of the type 3 G protein-coupled receptor family. Using STS analysis, Robbins et al. (2000) mapped the GPRC5B gene to 16p12.3-p12.1. Members of this superfamily are characterized by a signature 7-transmembrane domain motif. The encoded protein may modulate insulin secretion and increased protein expression is associated with type 2 diabetes. Alternative splicing results in multiple transcript variants.
Entrez Gene ID
Human51704
Mouse64297
Rat293546
UniProt ID
HumanQ9NZH0
MouseQ923Z0
RatD4A3F5
Alternative Names
G Protein-Coupled Receptor Class C Group 5 Member B; G Protein-Coupled Receptor, Class C, Group 5, Member B; G Protein-Coupled Receptor, Family C, Group 1, Member B; Retinoic Acid-Induced Gene 2 Protein; RAIG-2; RAIG2; G Protein-Coupled Receptor, Family C, Group 5, Member B; Retinoic Acid Responsive Gene Protein; A-69G12.1
Function
Unknown. This retinoic acid-inducible G-protein coupled receptor provide evidence for a possible interaction between retinoid and G-protein signaling pathways.
Biological Process
Positive regulation of canonical Wnt signaling pathway Source: CAFA
Positive regulation of I-kappaB kinase/NF-kappaB signaling Source: CAFA
Positive regulation of inflammatory response Source: CAFA
Positive regulation of macrophage cytokine production Source: CAFA
Positive regulation of neuron differentiation Source: CAFA
Positive regulation of protein tyrosine kinase activity Source: CAFA
Cellular Location
Cell membrane; Cytoplasmic vesicle membrane. Localized in the plasma membrane and perinuclear vesicles.
Topology
Extracellular: 29-56
Helical: 57-77
Cytoplasmic: 78-94
Helical: 95-115
Extracellular: 116-126
Helical: 127-147
Cytoplasmic: 148-162
Helical: 163-183
Extracellular: 184-199
Helical: 200-220
Cytoplasmic: 221-234
Helical: 235-255
Extracellular: 256-271
Helical: 272-292
Cytoplasmic: 293-403

Fu, W., Franchini, L., & Orlandi, C. (2022). Comprehensive Spatial Profile of the Orphan G Protein Coupled Receptor GPRC5B Expression in Mouse Brain. Frontiers in Neuroscience, 16, 891544.

Xu, W., Nelson-Maney, N. P., Bálint, L., Kwon, H. B., Davis, R. B., Dy, D. C., ... & Caron, K. M. (2022). Orphan G-Protein Coupled Receptor GPRC5B Is Critical for Lymphatic Development. International Journal of Molecular Sciences, 23(10), 5712.

Alonso-Gardón, M., Elorza-Vidal, X., Castellanos, A., La Sala, G., Armand-Ugon, M., Gilbert, A., ... & Estévez, R. (2021). Identification of the GlialCAM interactome: the G protein-coupled receptors GPRC5B and GPR37L1 modulate megalencephalic leukoencephalopathy proteins. Human Molecular Genetics, 30(17), 1649-1665.

Hirabayashi, Y., & Kim, Y. J. (2020). Roles of GPRC5 family proteins: focusing on GPRC5B and lipid-mediated signalling. The journal of biochemistry, 167(6), 541-547.

Carvalho, J., Chennupati, R., Li, R., Günther, S., Kaur, H., Zhao, W., ... & Wettschureck, N. (2020). Orphan G protein–coupled receptor GPRC5B controls smooth muscle contractility and differentiation by inhibiting prostacyclin receptor signaling. Circulation, 141(14), 1168-1183.

Zambrano, S., Möller-Hackbarth, K., Li, X., Rodriguez, P. Q., Charrin, E., Schwarz, A., ... & Patrakka, J. (2019). GPRC5B modulates inflammatory response in glomerular diseases via NF-κB pathway. Journal of the American Society of Nephrology, 30(9), 1573-1586.

von Samson-Himmelstjerna, F. A., Freundt, G., Nitz, J. T., Stelter, F., Luedde, M., Wieland, T., ... & Hippe, H. J. (2019). The orphan receptor GPRC5B modulates inflammatory and fibrotic pathways in cardiac fibroblasts and mice hearts. Biochemical and biophysical research communications, 514(4), 1198-1203.

Kim, Y. J., Greimel, P., & Hirabayashi, Y. (2018). GPRC5B-mediated sphingomyelin synthase 2 phosphorylation plays a critical role in insulin resistance. IScience, 8, 250-266.

Sano, T., Kohyama-Koganeya, A., Kinoshita, M. O., Tatsukawa, T., Shimizu, C., Oshima, E., ... & Hirabayashi, Y. (2018). Loss of GPRC5B impairs synapse formation of Purkinje cells with cerebellar nuclear neurons and disrupts cerebellar synaptic plasticity and motor learning. Neuroscience research, 136, 33-47.

Atanes, P., Ruz-Maldonado, I., Hawkes, R., Liu, B., Persaud, S. J., & Amisten, S. (2018). Identifying signalling pathways regulated by GPRC5B in β-Cells by CRISPR-Cas9-mediated genome editing. Cellular Physiology and Biochemistry, 45(2), 656-666.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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