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Mouse Anti-GRK1 Recombinant Antibody (CBLG1-1779) (CBMAB-G4984-LY)

This product is antibody recognizes GRK1. The antibody CBLG1-1779 immunoassay techniques such as: IF, IP, WB.
See all GRK1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBLG1-1779
Antibody Isotype
IgG
Application
IF, IP, WB

Basic Information

Immunogen
Full length human GRK1
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
G protein-coupled receptor kinase 1
Introduction
This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates rhodopsin and initiates its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2). [provided by RefSeq, Jul 2008]
Entrez Gene ID
6011
UniProt ID
Q15835
Alternative Names
G Protein-Coupled Receptor Kinase 1; Rhodopsin Kinase; EC 2.7.11.14; RHOK; RK; EC 2.7.11; GPRK1;
Function
Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade (PubMed:15946941).

This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination (By similarity).

May play a role in the maintenance of the outer nuclear layer in the retina (By similarity).
Biological Process
Protein autophosphorylation Source: UniProtKB
Protein phosphorylation Source: GO_Central
Regulation of G protein-coupled receptor signaling pathway Source: ProtInc
Regulation of rhodopsin mediated signaling pathway Source: UniProtKB
Regulation of signal transduction Source: GO_Central
Rhodopsin mediated signaling pathway Source: ProtInc
Visual perception Source: ProtInc
Cellular Location
Membrane; Photoreceptor outer segment. Subcellular location is not affected by light or dark conditions.
Involvement in disease
Night blindness, congenital stationary, Oguchi type 2 (CSNBO2):
A non-progressive retinal disorder characterized by impaired night vision, often associated with nystagmus and myopia. Congenital stationary night blindness Oguchi type is associated with fundus discoloration and abnormally slow dark adaptation.
PTM
Autophosphorylated, Ser-21 is a minor site of autophosphorylation compared to Ser-491 and Thr-492 (By similarity). Phosphorylation at Ser-21 is regulated by light and activated by cAMP.
Farnesylation is required for full activity.

Millette, M. A., Roy, S., & Salesse, C. (2022). Farnesylation and lipid unsaturation are critical for the membrane binding of the C-terminal segment of G-Protein Receptor Kinase 1. Colloids and Surfaces B: Biointerfaces, 211, 112315.

Gulati, S., & Palczewski, K. (2022). Structural view of G protein-coupled receptor signaling in the retinal rod outer segment. Trends in Biochemical Sciences.

Chen, Q., Plasencia, M., Li, Z., Mukherjee, S., Patra, D., Chen, C. L., ... & Tesmer, J. J. (2021). Structures of rhodopsin in complex with G-protein-coupled receptor kinase 1. Nature, 595(7868), 600-605.

Sulon, S. M., & Benovic, J. L. (2021). Targeting G protein–coupled receptor kinases to G protein–coupled receptors. Current opinion in endocrine and metabolic research, 16, 56-65.

Benovic, J. L. (2021). Historical perspective of the G protein-coupled receptor kinase family. Cells, 10(3), 555.

Kolesnikov, A. V., Chrispell, J. D., Osawa, S., Kefalov, V. J., & Weiss, E. R. (2020). Phosphorylation at Serine 21 in G protein-coupled receptor kinase 1 (GRK1) is required for normal kinetics of dark adaption in rod but not cone photoreceptors. FASEB journal: official publication of the Federation of American Societies for Experimental Biology, 34(2), 2677.

Yu, S., Sun, L., Jiao, Y., & Lee, L. T. O. (2018). The role of G protein-coupled receptor kinases in cancer. International journal of biological sciences, 14(2), 189.

Komolov, K. E., & Benovic, J. L. (2018). G protein-coupled receptor kinases: Past, present and future. Cellular signalling, 41, 17-24.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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