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Mouse Anti-GTF2B Recombinant Antibody (CB433A) (CBMAB-CA433LY)

The product is antibody recognizes GTF2B. The antibody CB433A immunoassay techniques such as: ELISA, WB, IHC, ICC.
See all GTF2B antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Pig, Rat, Dog
Clone
CB433A
Antibody Isotype
IgG2b
Application
ELISA, WB, IHC, ICC

Basic Information

Immunogen
Recombinant full length protein (316aa) of Human GTF2B.
Specificity
Human, Mouse, Pig, Rat, Dog
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
general transcription factor IIB
Introduction
This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. [provided by RefSeq, Jul 2008]
Entrez Gene ID
UniProt ID
Alternative Names
General Transcription Factor IIB; General Transcription Factor TFIIB; S300-II; TFIIB; TF2B; RNA Polymerase II Transcription Factor IIB; Transcription Initiation Factor IIB;
Function
General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA (PubMed:1876184, PubMed:1946368, PubMed:1517211, PubMed:3818643, PubMed:3029109, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312, PubMed:7601352, PubMed:9420329, PubMed:12931194, PubMed:27193682).

Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex (PubMed:8504927, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312).

Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle (PubMed:7601352).

Associates with chromatin to core promoter-specific regions (PubMed:12931194, PubMed:24441171).

Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element (PubMed:9420329, PubMed:16230532, PubMed:7675079, PubMed:10619841).

Modulates transcription start site selection (PubMed:10318856).

Exhibits also autoacetyltransferase activity that contributes to the activated transcription (PubMed:12931194).
Biological Process
DNA-templated transcription, initiation Source: GO_Central
Positive regulation of core promoter binding Source: GO_Central
Protein acetylation Source: UniProtKB
RNA polymerase II core complex assembly Source: UniProtKB
RNA polymerase II preinitiation complex assembly Source: UniProtKB
Transcriptional start site selection at RNA polymerase II promoter Source: UniProtKB
Transcription by RNA polymerase II Source: ARUK-UCL
Transcription initiation from RNA polymerase II promoter Source: UniProtKB
Viral transcription Source: GO_Central
Cellular Location
Nucleus; Chromosome. Non-acetylated form colocalizes with DNA in the G0/1, S and G2 phases of the cell cycle, but not during mitosis (PubMed:24441171). Acetylated form colocalizes at transcriptionally silent mitotic chromatids during mitosis at metaphase, anaphase, and telophase phases of the cell cycle (PubMed:24441171).
PTM
Acetylated (PubMed:24441171). Autoacetylated; autoacetylation at Lys-238 stimulates transcription activation (PubMed:12931194).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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