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Mouse Anti-HERC2 (AA 1781-1974) Recombinant Antibody (17/HERC2) (CBMAB-H1865-FY)

This product is mouse antibody that recognizes HERC2. The antibody 17/HERC2 can be used for immunoassay techniques such as: WB.
See all HERC2 antibodies

Summary

Host Animal
Mouse
Specificity
Mouse, Human, Rat
Clone
17/HERC2
Antibody Isotype
IgG1
Application
WB

Basic Information

Immunogen
Mouse HERC2 aa. 1781-1974
Specificity
Mouse, Human, Rat
Antibody Isotype
IgG1
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Concentration
0.25 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 1781-1974

Target

Full Name
HECT And RLD Domain Containing E3 Ubiquitin Protein Ligase 2
Introduction
This gene belongs to the HERC gene family that encodes a group of unusually large proteins, which contain multiple structural domains. All members have at least 1 copy of an N-terminal region showing homology to the cell cycle regulator RCC1 and a C-terminal HECT (homologous to E6-AP C terminus) domain found in a number of E3 ubiquitin protein ligases. Genetic variations in this gene are associated with skin/hair/eye pigmentation variability. Multiple pseudogenes of this gene are located on chromosomes 15 and 16.
Entrez Gene ID
Human8924
Mouse15204
Rat308669
UniProt ID
HumanO95714
MouseQ4U2R1
RatD4ACN3
Alternative Names
HECT And RLD Domain Containing E3 Ubiquitin Protein Ligase 2; HECT-Type E3 Ubiquitin Transferase HERC2; Hect Domain And RLD 2; Hect Domain And RCC1-Like Domain-Containing Protein 2; HECT Domain And RCC1-Like Domain-Containing Protein 2; Probable E3 Ubiquitin-Protein Ligase HERC2; E3 Ubiquitin-Protein Ligase HERC2; EC 2.3.2.26
Function
E3 ubiquitin-protein ligase that regulates ubiquitin-dependent retention of repair proteins on damaged chromosomes. Recruited to sites of DNA damage in response to ionizing radiation (IR) and facilitates the assembly of UBE2N and RNF8 promoting DNA damage-induced formation of 'Lys-63'-linked ubiquitin chains. Acts as a mediator of binding specificity between UBE2N and RNF8. Involved in the maintenance of RNF168 levels. E3 ubiquitin-protein ligase that promotes the ubiquitination and proteasomal degradation of XPA which influences the circadian oscillation of DNA excision repair activity. By controlling the steady-state expression of the IGF1R receptor, indirectly regulates the insulin-like growth factor receptor signaling pathway (PubMed:26692333).
Biological Process
Cellular response to DNA damage stimulus Source: UniProtKB
DNA repair Source: UniProtKB-KW
Intracellular protein transport Source: UniProtKB
Proteasome-mediated ubiquitin-dependent protein catabolic process Source: Ensembl
Protein ubiquitination Source: UniProtKB
Spermatogenesis Source: Ensembl
Cellular Location
Centriole; Nucleus; Cytoplasm. Recruited to sites of DNA damage in response to ionizing radiation (IR) via its interaction with RNF8. May loose association with centrosomes during mitosis.
Involvement in disease
Mental retardation, autosomal recessive 38 (MRT38):
A disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period. MRT38 is characterized by global developmental delay affecting motor, speech, adaptive, and social development. Patients manifest autistic features, aggression, self-injury, impulsivity, and distractibility.
PTM
Phosphorylation at Thr-4827 is required for interaction with RNF8.
Sumoylated with SUMO1 by PIAS4 in response to double-strand breaks (DSBs), promoting the interaction with RNF8.

Anandhan, A., Dodson, M., Shakya, A., Chen, J., Liu, P., Wei, Y., ... & Zhang, D. D. (2023). NRF2 controls iron homeostasis and ferroptosis through HERC2 and VAMP8. Science Advances, 9(5), eade9585.

Liu, Y., Xu, Q., Deng, F., Zheng, Z., Luo, J., Wang, P., ... & Zuo, D. (2023). HERC2 promotes inflammation-driven cancer stemness and immune evasion in hepatocellular carcinoma by activating STAT3 pathway. Journal of Experimental & Clinical Cancer Research, 42(1), 1-20.

Tencer, A. H., Liu, J., Zhu, J., Burkholder, N. T., Zhang, Y., Wu, W., ... & Kutateladze, T. G. (2022). The ZZ domain of HERC2 is a receptor of arginylated substrates. Scientific Reports, 12(1), 6063.

Salvo, N. M., Mathisen, M. G., Janssen, K., Berg, T., & Olsen, G. H. (2022). Experimental long-distance haplotyping of OCA2-HERC2 variants. Forensic Science International: Genetics Supplement Series, 8, 188-190.

Mathieu, N. A., Levin, R. H., & Spratt, D. E. (2021). Exploring the roles of HERC2 and the NEDD4L HECT E3 ubiquitin ligase subfamily in p53 signaling and the DNA damage response. Frontiers in Oncology, 11, 659049.

García‐Cano, J., Sánchez‐Tena, S., Sala‐Gaston, J., Figueras, A., Viñals, F., Bartrons, R., ... & Rosa, J. L. (2020). Regulation of the MDM2‐p53 pathway by the ubiquitin ligase HERC2. Molecular Oncology, 14(1), 69-86.

Lai, Y., Zhu, M., Wu, W., Rokutanda, N., Togashi, Y., Liang, W., & Ohta, T. (2019). HERC2 regulates RPA2 by mediating ATR-induced Ser33 phosphorylation and ubiquitin-dependent degradation. Scientific reports, 9(1), 14257.

Abraham, J. R., Barnard, J., Wang, H., Noritz, G. H., Yeganeh, M., Buhas, D., & Natowicz, M. R. (2019). Proteomic investigations of human HERC2 mutants: Insights into the pathobiology of a neurodevelopmental disorder. Biochemical and biophysical research communications, 512(2), 421-427.

Wu, W., Rokutanda, N., Takeuchi, J., Lai, Y., Maruyama, R., Togashi, Y., ... & Ohta, T. (2018). HERC2 facilitates BLM and WRN helicase complex interaction with RPA to suppress G-quadruplex DNA. Cancer Research, 78(22), 6371-6385.

Wang, L. Q., Zheng, Y. Y., Zhou, H. J., Zhang, X. X., Wu, P., & Zhu, S. M. (2021). LncRNA-Fendrr protects against the ubiquitination and degradation of NLRC4 protein through HERC2 to regulate the pyroptosis of microglia. Molecular Medicine, 27(1), 1-10.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

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