Mouse Anti-HTRA3 (AA 344-453) Recombinant Antibody (CBFYH-2527) (CBMAB-H3541-FY)

Name: Mouse Anti-HTRA3 (AA 344-453) Recombinant Antibody (CBFYH-2527)
SKU: CBMAB-H3541-FY
Rating: 5 (1 reviews)

See all HTRA3 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYH-2527

Application

ELISA, WB

Immunogen

Recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Immunogen sequence: FQDKQIKDWK KRFIGIRMRT ITPSLVDELK ASNPDFPEVS SGIYVQEVAP NSPSQRGGIQ DGDIIVKVNG RPLVDSSELQ EAVLTESPLL LEVRRGNDDL LFSIAPEVVM

Specificity

Human

Antibody Isotype

IgG2a, κ

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 344-453

More Infomation

Target

Full Name

HtrA serine peptidase 3

Introduction

HTRA3 (HtrA Serine Peptidase 3) is a Protein Coding gene. Diseases associated with HTRA3 include Ovary Serous Adenocarcinoma and Ovarian Cystadenocarcinoma. Gene Ontology (GO) annotations related to this gene include serine-type endopeptidase activity and endopeptidase activity. An important paralog of this gene is HTRA1.

Entrez Gene ID

94031

UniProt ID

P83110

Alternative Names

HtrA Serine Peptidase 3; Pregnancy-Related Serine Protease; High-Temperature Requirement Factor A3; Prsp; Probable Serine Protease HTRA3; Serine Protease HTRA3

Function

Serine protease that cleaves beta-casein/CSN2 as well as several extracellular matrix (ECM) proteoglycans such as decorin/DCN, biglycan/BGN and fibronectin/FN1. Inhibits signaling mediated by TGF-beta family proteins possibly indirectly by degradation of these ECM proteoglycans (By similarity).

May act as a tumor suppressor. Negatively regulates, in vitro, trophoblast invasion during placental development and may be involved in the development of the placenta in vivo. May also have a role in ovarian development, granulosa cell differentiation and luteinization (PubMed:21321049, PubMed:22229724).

Biological Process

Negative regulation of BMP signaling pathway Source: UniProtKB
Negative regulation of transforming growth factor beta receptor signaling pathway Source: UniProtKB
Proteolysis Source: UniProtKB

Cellular Location

Secreted. Secretion increased during decidualization of endometrial stromal cells.

Xu, Q., Wang, J., Wang, J., Zhu, Y., Chen, B., Guo, R., ... & Liu, F. (2023). A pan-cancer analysis to provide insight into the immunological role and prognostic value of HTRA3. Critical Reviews™ in Eukaryotic Gene Expression, 33.

Ko, T., Nomura, S., Yamada, S., Fujita, K., Fujita, T., Satoh, M., ... & Komuro, I. (2022). Cardiac fibroblasts regulate the development of heart failure via Htra3-TGF-β-IGFBP7 axis. Nature communications, 13(1), 3275.

Guo, Y., Ma, S., Xu, M., Wei, Y., Zhang, X., Huang, Y., ... & Deng, X. (2021). HtrA3‐Mediated Endothelial Cell–Extracellular Matrix Crosstalk Regulates Tip Cell Specification. Advanced Functional Materials, 31(30), 2100633.

Acharya, S., Dutta, S., & Bose, K. (2020). A distinct concerted mechanism of structural dynamism defines activity of human serine protease HtrA3. Biochemical Journal, 477(2), 407-429.

Ji, C., Sun, L. S., Xing, F., Niu, N., Gao, H. L., Dai, J. W., ... & Jiang, B. C. (2020). HTRA3 is a prognostic biomarker and associated with immune infiltrates in gastric cancer. Frontiers in oncology, 10, 603480.

Wenta, T., Rychlowski, M., Jurewicz, E., Jarzab, M., Zurawa‐Janicka, D., Filipek, A., & Lipinska, B. (2019). The HtrA3 protease promotes drug‐induced death of lung cancer cells by cleavage of the X‐linked inhibitor of apoptosis protein (XIAP). The FEBS Journal, 286(22), 4579-4596.

Zhao, J., Feng, M., Liu, D., Liu, H., Shi, M., Zhang, J., & Qu, J. (2019). Antagonism between HTRA3 and TGFβ1 Contributes to Metastasis in Non–Small Cell Lung Cancer. Cancer Research, 79(11), 2853-2864.

Wenta, T., Zurawa-Janicka, D., Rychlowski, M., Jarzab, M., Glaza, P., Lipinska, A., ... & Lipinska, B. (2018). HtrA3 is a cellular partner of cytoskeleton proteins and TCP1α chaperonin. Journal of proteomics, 177, 88-111.

Lv, Q., Yang, B., Ning, C., Xie, B., Nie, G., Chen, X., & Chen, Q. (2018). Hypoxia is involved in the reduction of HtrA3 in patients with endometrial hyperplasia and cancer. Biochemical and biophysical research communications, 503(4), 2918-2923.

Wang, Y., Li, Y., Hyett, J., da Silva Costa, F., & Nie, G. (2018). HtrA3 isoform–specific ELISAs for early detection of preeclampsia. SLAS DISCOVERY: Advancing Life Sciences R&D, 23(10), 1092-1099.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-HTRA3 (AA 344-453) Recombinant Antibody (CBFYH-2527)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry