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Mouse Anti-ILF2 Recombinant Antibody (1E2) (CBMAB-A4450-LY)

The product is antibody recognizes ILF2. The antibody 1E2 immunoassay techniques such as: WB, ELISA.
See all ILF2 antibodies
Published Data
Fig. 1 Wholecell lysates of control and LiCl-treated (25 mM for 1 h) cells were immunoprecipitated with antibodies against proteins of interest.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
1E2
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
ILF2 (NP_004506, 151 a.a. ~ 250 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
interleukin enhancer binding factor 2, 45kDa
Introduction
Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of the interleukin 2 gene. NFAT binds to a sequence in the interleukin 2 gene enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the smaller of which is the product of this gene. The encoded protein binds strongly to the 90 kDa protein and stimulates its ability to enhance gene expression. [provided by RefSeq]
Entrez Gene ID
3608
UniProt ID
Q12905
Alternative Names
MGC8391; NF45; PRO3063
Function
Chromatin-interacting protein that forms a stable heterodimer with interleukin enhancer-binding factor 3/ILF3 and plays a role in several biological processes including transcription, innate immunity or cell growth (PubMed:18458058, PubMed:31212927).
Essential for the efficient reshuttling of ILF3 (isoform 1 and isoform 2) into the nucleus. Together with ILF3, forms an RNA-binding complex that is required for mitotic progression and cytokinesis by regulating the expression of a cluster of mitotic genes. Mechanistically, competes with STAU1/STAU2-mediated mRNA decay (PubMed:32433969).
Plays also a role in the inhibition of various viruses including Japanese encephalitis virus or enterovirus 71.
(Microbial infection) Plays a positive role in HIV-1 virus production by binding to and thereby stabilizing HIV-1 RNA, together with ILF3.
Biological Process
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Nucleus, nucleolus; Cytoplasm; Nucleus. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
PTM
Ubiquitinated at Lys-45 by CRBN with polyubiquitin chains by the CUL4-RING E3 ligase (CRL4-CRBN) and then degraded by the proteasome.

Xu, J., Li, F., Zhang, J., Wang, L., Liu, X., Xue, W., ... & Cui, Y. (2023). The interleukin enhancer binding factor-2 (ILF2) gene in Penaeus japonicus shrimp: cloning, tissue distribution, and antiviral effects. Aquaculture International, 1-15.

Zhang, H., Che, Y., Xuan, B., Wu, X., & Li, H. (2022). Serine hydroxymethyltransferase 2 (SHMT2) potentiates the aggressive process of oral squamous cell carcinoma by binding to interleukin enhancer-binding factor 2 (ILF2). Bioengineered, 13(4), 8785-8797.

Wei, L., Yang, C., Wang, G., Li, K., Zhang, Y., Guan, H., ... & Zhong, C. (2021). Interleukin enhancer binding factor 2 regulates cell viability and apoptosis of human brain vascular smooth muscle cells. Journal of Molecular Neuroscience, 71, 225-233.

Zhang, X., Bustos, M. A., Gross, R., Ramos, R. I., Takeshima, T. L., Mills, G. B., ... & Hoon, D. S. (2021). Interleukin enhancer‐binding factor 2 promotes cell proliferation and DNA damage response in metastatic melanoma. Clinical and Translational Medicine, 11(10), e608.

Cheung, Y., Wu, Z., Garcia-Barcelo, M. M., Tam, P. K. H., Ma, A. C. H., & Lui, V. C. H. (2021). Deletion of interleukin enhancer binding factor 2 (ILF2) resulted in defective biliary development and bile flow blockage. Journal of Pediatric Surgery, 56(2), 352-359.

Shiu, T. Y., Lin, H. H., Shih, Y. L., Feng, A. C., Huang, H. H., Huang, T. Y., ... & Hsieh, T. Y. (2021). CRNDE-h transcript/miR-136-5p axis regulates interleukin enhancer binding factor 2 expression to promote hepatocellular carcinoma cell proliferation. Life Sciences, 284, 119708.

Jiang, X., Zhang, X., Ren, C., Ruan, Y., Lu, Y., Yuan, L., ... & Chen, T. (2021). Interleukin-2 enhancer binding factor 2 (ILF2) in pacific white shrimp (Litopenaeus vannamei): Alternatively spliced isoforms with different responses in the immune defenses against vibrio infection. Developmental & Comparative Immunology, 118, 103975.

Cui, X., Qian, P., Rao, T., Wei, Y., Zhao, F., Zhang, H., ... & Li, X. (2019). Cellular interleukin enhancer-binding factor 2, ILF2, inhibits Japanese Encephalitis virus replication in vitro. Viruses, 11(6), 559.

Jin, J., Wang, W., Ai, S., Liu, W., Song, Y., Luo, Z., ... & Wu, J. (2019). Enterovirus 71 represses interleukin enhancer-binding factor 2 production and nucleus translocation to antagonize ILF2 antiviral effects. Viruses, 12(1), 22.

Jin, Z., Xu, L., Zhang, L., Zhao, M., Li, D., Ye, L., ... & Chen, B. (2018). Interleukin enhancer binding factor 2 is a prognostic biomarker for breast cancer that also predicts neoadjuvant chemotherapy responses. American journal of translational research, 10(6), 1677.

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For research use only. Not intended for any clinical use.

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