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Mouse Anti-ILF2 Recombinant Antibody (6F1) (CBMAB-N0880-WJ)

This product is a Mouse antibody that recognizes ILF2. The antibody 6F1 can be used for immunoassay techniques such as: IF, IHC-P, WB.
See all ILF2 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
6F1
Antibody Isotype
IgG1
Application
IF, IHC-P, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.3, 1% BSA, 50% glycerol
Preservative
0.02% sodium azide
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
interleukin enhancer binding factor 2, 45kDa
Introduction
The protein encoded by this gene is a transcription factor required for T-cell expression of the interleukin 2 gene. It also binds RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. The encoded 45 kDa protein (NF45, ILF2) forms a complex with the 90 kDa interleukin enhancer-binding factor 3 (NF90, ILF3), and this complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm, to repair DNA breaks by nonhomologous end joining, and to negatively regulate the microRNA processing pathway. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. Alternative splicing results in multiple transcript variants. Related pseudogenes have been found on chromosomes 3 and 14. [provided by RefSeq, Dec 2014]
Entrez Gene ID
3608
UniProt ID
Q12905
Alternative Names
Interleukin Enhancer Binding Factor 2
Function
Chromatin-interacting protein that forms a stable heterodimer with interleukin enhancer-binding factor 3/ILF3 and plays a role in several biological processes including transcription, innate immunity or cell growth (PubMed:18458058, PubMed:31212927).
Essential for the efficient reshuttling of ILF3 (isoform 1 and isoform 2) into the nucleus. Together with ILF3, forms an RNA-binding complex that is required for mitotic progression and cytokinesis by regulating the expression of a cluster of mitotic genes. Mechanistically, competes with STAU1/STAU2-mediated mRNA decay (PubMed:32433969).
Plays also a role in the inhibition of various viruses including Japanese encephalitis virus or enterovirus 71.
(Microbial infection) Plays a positive role in HIV-1 virus production by binding to and thereby stabilizing HIV-1 RNA, together with ILF3.
Biological Process
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Nucleus, nucleolus; Cytoplasm; Nucleus. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
PTM
Ubiquitinated at Lys-45 by CRBN with polyubiquitin chains by the CUL4-RING E3 ligase (CRL4-CRBN) and then degraded by the proteasome.

Xu, J., Li, F., Zhang, J., Wang, L., Liu, X., Xue, W., ... & Cui, Y. (2023). The interleukin enhancer binding factor-2 (ILF2) gene in Penaeus japonicus shrimp: cloning, tissue distribution, and antiviral effects. Aquaculture International, 1-15.

Zhang, H., Che, Y., Xuan, B., Wu, X., & Li, H. (2022). Serine hydroxymethyltransferase 2 (SHMT2) potentiates the aggressive process of oral squamous cell carcinoma by binding to interleukin enhancer-binding factor 2 (ILF2). Bioengineered, 13(4), 8785-8797.

Wei, L., Yang, C., Wang, G., Li, K., Zhang, Y., Guan, H., ... & Zhong, C. (2021). Interleukin enhancer binding factor 2 regulates cell viability and apoptosis of human brain vascular smooth muscle cells. Journal of Molecular Neuroscience, 71, 225-233.

Zhang, X., Bustos, M. A., Gross, R., Ramos, R. I., Takeshima, T. L., Mills, G. B., ... & Hoon, D. S. (2021). Interleukin enhancer‐binding factor 2 promotes cell proliferation and DNA damage response in metastatic melanoma. Clinical and Translational Medicine, 11(10), e608.

Cheung, Y., Wu, Z., Garcia-Barcelo, M. M., Tam, P. K. H., Ma, A. C. H., & Lui, V. C. H. (2021). Deletion of interleukin enhancer binding factor 2 (ILF2) resulted in defective biliary development and bile flow blockage. Journal of Pediatric Surgery, 56(2), 352-359.

Shiu, T. Y., Lin, H. H., Shih, Y. L., Feng, A. C., Huang, H. H., Huang, T. Y., ... & Hsieh, T. Y. (2021). CRNDE-h transcript/miR-136-5p axis regulates interleukin enhancer binding factor 2 expression to promote hepatocellular carcinoma cell proliferation. Life Sciences, 284, 119708.

Jiang, X., Zhang, X., Ren, C., Ruan, Y., Lu, Y., Yuan, L., ... & Chen, T. (2021). Interleukin-2 enhancer binding factor 2 (ILF2) in pacific white shrimp (Litopenaeus vannamei): Alternatively spliced isoforms with different responses in the immune defenses against vibrio infection. Developmental & Comparative Immunology, 118, 103975.

Cui, X., Qian, P., Rao, T., Wei, Y., Zhao, F., Zhang, H., ... & Li, X. (2019). Cellular interleukin enhancer-binding factor 2, ILF2, inhibits Japanese Encephalitis virus replication in vitro. Viruses, 11(6), 559.

Jin, J., Wang, W., Ai, S., Liu, W., Song, Y., Luo, Z., ... & Wu, J. (2019). Enterovirus 71 represses interleukin enhancer-binding factor 2 production and nucleus translocation to antagonize ILF2 antiviral effects. Viruses, 12(1), 22.

Jin, Z., Xu, L., Zhang, L., Zhao, M., Li, D., Ye, L., ... & Chen, B. (2018). Interleukin enhancer binding factor 2 is a prognostic biomarker for breast cancer that also predicts neoadjuvant chemotherapy responses. American journal of translational research, 10(6), 1677.

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For research use only. Not intended for any clinical use.

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