Mouse Anti-MDM4 Recombinant Antibody (CBT873) (V2LY-0625-LY3211)

Name: Mouse Anti-MDM4 Recombinant Antibody (CBT873)
SKU: V2LY-0625-LY3211
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-MDM4 Recombinant Antibody (CBT873) (V2LY-0625-LY3211) in IF

Mouse Anti-MDM4 Recombinant Antibody (CBT873) (V2LY-0625-LY3211) in IHC

Mouse Anti-MDM4 Recombinant Antibody (CBT873) (V2LY-0625-LY3211) in WB

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT873

Application

WB, IHC-P, IF, ICC, ELISA

Immunogen

Purified recombinant fragment of human MDMX expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:10,000
IF	1:200-1:1,000
IHC	1:200-1:1,000
WB	1:500-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Buffer

BSA, Glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Minichromosome Maintenance Complex Component 4

Entrez Gene ID

4194

UniProt ID

O15151

Function

Along with MDM2, contributes to TP53 regulation (PubMed:32300648).

Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions.

Biological Process

Atrial septum development Source: BHF-UCL
Atrioventricular valve morphogenesis Source: BHF-UCL
Cellular response to hypoxia Source: UniProtKB
DNA damage response, signal transduction by p53 class mediator Source: UniProtKB
Endocardial cushion morphogenesis Source: BHF-UCL
Heart valve development Source: BHF-UCL
Negative regulation of apoptotic process Source: InterPro
Negative regulation of cell population proliferation Source: ProtInc
Negative regulation of protein catabolic process Source: UniProtKB
Negative regulation of transcription, DNA-templated Source: ComplexPortal
Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Protein-containing complex assembly Source: UniProtKB
Protein stabilization Source: UniProtKB
Regulation of cell cycle Source: InterPro
Regulation of transcription by RNA polymerase II Source: GO_Central
Ventricular septum development Source: BHF-UCL

Cellular Location

Nucleus

Involvement in disease

Bone marrow failure syndrome 6 (BMFS6):
A form of bone marrow failure syndrome, a heterogeneous group of life-threatening disorders characterized by hematopoietic defects in association with a range of variable extra-hematopoietic manifestations. BMFS6 is an autosomal dominant form characterized by intermittent neutropenia, lymphopenia, or anemia associated with hypocellular bone marrow, and increased susceptibility to cancer.

PTM

Phosphorylated. Phosphorylation at Ser-367 promotes interaction with YWHAG and subsequent ubiquitination and degradation. Phosphorylation at Ser-342 also induces ubiquitination and degradation but to a lower extent.
Ubiquitinated and degraded by MDM2. Deubiquitination by USP2 on the other hand stabilizes the MDM4 protein.

Sanford, J. D., Yang, J., Han, J., Tollini, L. A., Jin, A., & Zhang, Y. (2021). MDMX is essential for the regulation of p53 protein levels in the absence of a functional MDM2 C-terminal tail. BMC Molecular and Cell Biology, 22, 1-9.

Wu, J., Lu, G., & Wang, X. (2021). MDM4 alternative splicing and implication in MDM4 targeted cancer therapies. American Journal of Cancer Research, 11(12), 5864.

Zhang, S., Lou, J., Li, Y., Zhou, F., Yan, Z., Lyu, X., & Zhao, Y. (2021). Recent progress and clinical development of inhibitors that block MDM4/p53 protein–protein interactions. Journal of Medicinal Chemistry, 64(15), 10621-10640.

Eskandari, M., Shi, Y., Liu, J., Albanese, J., Goel, S., Verma, A., & Wang, Y. (2021). The expression of MDM2, MDM4, p53 and p21 in myeloid neoplasms and the effect of MDM2/MDM4 dual inhibitor. Leukemia & Lymphoma, 62(1), 167-175.

Wohlberedt, K., Klusmann, I., Derevyanko, P. K., Henningsen, K., Choo, J. A. M. Y., Manzini, V., ... & Dobbelstein, M. (2020). Mdm4 supports DNA replication in a p53-independent fashion. Oncogene, 39(25), 4828-4843.

Liu, T., Zhang, H., Yi, S., Gu, L., & Zhou, M. (2019). Mutual regulation of MDM 4 and TOP 2A in cancer cell proliferation. Molecular oncology, 13(5), 1047-1058.

Haupt, S., Mejía-Hernández, J. O., Vijayakumaran, R., Keam, S. P., & Haupt, Y. (2019). The long and the short of it: the MDM4 tail so far. Journal of molecular cell biology, 11(3), 231-244.

Karki, A., Putra, J., Kim, S. S., Laquaglia, M. J., Perez‑Atayde, A. R., Sadri‑Vakili, G., & Vakili, K. (2019). MDM4 expression in fibrolamellar hepatocellular carcinoma. Oncology Reports, 42(4), 1487-1496.

Frosi, Y., Inoue, K., Ramlan, S. R., Lane, D. P., Watanabe, T., & Brown, C. J. (2019). Simultaneous measurement of p53: Mdm2 and p53: Mdm4 protein-protein interactions in whole cells using fluorescence labelled foci. Scientific Reports, 9(1), 17933.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

MDM4 Matched Antibody Pair (727) (CAT#: APMAB-727LY)

MDM4 Matched Antibody Pair (728) (CAT#: APMAB-728LY)

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Isotype control

For research use only. Not intended for any clinical use.

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