Mouse Anti-MKI67 Recombinant Antibody (13B444) (CBMAB-K2120-LY)

Name: Mouse Anti-MKI67 Recombinant Antibody (13B444)
SKU: CBMAB-K2120-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

13B444

Application

ELISA, WB

Immunogen

Purified recombinant fragment of Ki167 (aa3118-3256) expressed in E. coli

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Preservative

0.03% sodium azide

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

marker of proliferation Ki-67

Introduction

This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]

Entrez Gene ID

4288

UniProt ID

P46013

Alternative Names

Marker Of Proliferation Ki-67; Antigen Identified By Monoclonal Antibody Ki-67; Protein Phosphatase 1; Regulatory Subunit 105; Antigen KI-67; Antigen Ki67; Proliferation-Related Ki-67 Antigen;

Function

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226).

Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226).

Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226).

Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551).

Does not contribute to the internal structure of mitotic chromosomes (By similarity).

May play a role in chromatin organization (PubMed:24867636).

It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).

Biological Process

Cell cycle Source: UniProtKB-KW
Cell population proliferation Source: ProtInc
Regulation of chromatin organization Source: UniProtKB
Regulation of chromosome segregation Source: UniProtKB
Regulation of mitotic nuclear division Source: UniProtKB

Cellular Location

Nucleus
nucleolus
Other locations
Chromosome
Note: Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106).

PTM

Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA.

Andrés-Sánchez, N., Fisher, D., & Krasinska, L. (2022). Physiological functions and roles in cancer of the proliferation marker Ki-67. Journal of Cell Science, 135(11), jcs258932.

Mrouj, K., Andrés-Sánchez, N., Dubra, G., Singh, P., Sobecki, M., Chahar, D., ... & Fisher, D. (2021). Ki-67 regulates global gene expression and promotes sequential stages of carcinogenesis. Proceedings of the National Academy of Sciences, 118(10), e2026507118.

Davey, M. G., Hynes, S. O., Kerin, M. J., Miller, N., & Lowery, A. J. (2021). Ki-67 as a prognostic biomarker in invasive breast cancer. Cancers, 13(17), 4455.

Uxa, S., Castillo-Binder, P., Kohler, R., Stangner, K., Müller, G. A., & Engeland, K. (2021). Ki-67 gene expression. Cell Death & Differentiation, 28(12), 3357-3370.

Li, Z., Li, F., Pan, C., He, Z., Pan, X., Zhu, Q., ... & Chen, L. (2021). Tumor cell proliferation (Ki-67) expression and its prognostic significance in histological subtypes of lung adenocarcinoma. Lung Cancer, 154, 69-75.

Li, C., Zheng, M., Zheng, X., Fang, X., Dong, J., Wang, C., & Wang, T. (2021). Predictive Ki-67 proliferation index of cervical squamous cell carcinoma based on IVIM-DWI combined with texture features. Contrast Media & Molecular Imaging, 2021.

Xiong, D. D., Zeng, C. M., Jiang, L., Luo, D. Z., & Chen, G. (2019). Ki-67/MKI67 as a predictive biomarker for clinical outcome in gastric cancer patients: an updated meta-analysis and systematic review involving 53 studies and 7078 patients. Journal of Cancer, 10(22), 5339.

Menon, S. S., Guruvayoorappan, C., Sakthivel, K. M., & Rasmi, R. R. (2019). Ki-67 protein as a tumour proliferation marker. Clinica chimica acta, 491, 39-45.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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