Mouse Anti-NME1 Recombinant Antibody (CBWJN-1444) (CBMAB-N2722-WJ)

Name: Mouse Anti-NME1 Recombinant Antibody (CBWJN-1444)
SKU: CBMAB-N2722-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBWJN-1444

Application

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, Trehalose

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

NME/NM23 Nucleoside Diphosphate Kinase 1

Introduction

This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Jul 2008]

Entrez Gene ID

Human	4830
Mouse	18102
Rat	191575

UniProt ID

Human	P15531
Mouse	P15532
Rat	Q05982

Alternative Names

NME/NM23 Nucleoside Diphosphate Kinase 1; Non-Metastatic Cells 1, Protein (NM23A) Expressed In; Tumor Metastatic Process-Associated Protein; Metastasis Inhibition Factor Nm23; Granzyme A-Activated DNase; NDP Kinase A; EC 2.7.4.6; NM23-H1; NDPKA; GAAD;

Function

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. During GZMA-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.

Biological Process

Cell differentiationIEA:UniProtKB-KW
CTP biosynthetic processIEA:InterPro
EndocytosisIEA:UniProtKB-KW
GTP biosynthetic processIEA:InterPro
LactationIEA:Ensembl
Negative regulation of cell population proliferationManual Assertion Based On ExperimentTAS:UniProtKB
Nervous system developmentIEA:UniProtKB-KW
Positive regulation of DNA bindingManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of epithelial cell proliferationManual Assertion Based On ExperimentIMP:HGNC-UCL
Regulation of apoptotic processManual Assertion Based On ExperimentTAS:UniProtKB
UTP biosynthetic processIEA:InterPro

Cellular Location

Cytoplasm
Nucleus
Cell-cycle dependent nuclear localization which can be induced by interaction with Epstein-barr viral proteins or by degradation of the SET complex by GzmA.

Iuso, D., Garcia-Saez, I., Couté, Y., Yamaryo-Botté, Y., Boeri Erba, E., Adrait, A., ... & Khochbin, S. (2023). Nucleoside diphosphate kinases 1 and 2 regulate a protective liver response to a high-fat diet. Science Advances, 9(36), eadh0140.

Adam, K., Lesperance, J., Hunter, T., & Zage, P. E. (2020). The potential functional roles of NME1 histidine kinase activity in neuroblastoma pathogenesis. International journal of molecular sciences, 21(9), 3319.

Adam, K., Ning, J., Reina, J., & Hunter, T. (2020). NME/NM23/NDPK and histidine phosphorylation. International journal of molecular sciences, 21(16), 5848.

Mátyási, B., Farkas, Z., Kopper, L., Sebestyén, A., Boissan, M., Mehta, A., & Takács-Vellai, K. (2020). The function of NM23-H1/NME1 and its homologs in major processes linked to metastasis. Pathology & Oncology Research, 26, 49-61.

Radić, M., Šoštar, M., Weber, I., Ćetković, H., Slade, N., & Herak Bosnar, M. (2020). The subcellular localization and oligomerization preferences of NME1/NME2 upon radiation-induced DNA damage. International Journal of Molecular Sciences, 21(7), 2363.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

NME1 Matched Antibody Pair (803) (CAT#: APMAB-803LY)

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Isotype control

For research use only. Not intended for any clinical use.

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