Mouse Anti-NME1 Recombinant Antibody (CBT946) (V2LY-0625-LY3159)

Name: Mouse Anti-NME1 Recombinant Antibody (CBT946)
SKU: V2LY-0625-LY3159
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-NME1 Recombinant Antibody (CBT946) (V2LY-0625-LY3159) in FC

Mouse Anti-NME1 Recombinant Antibody (CBT946) (V2LY-0625-LY3159) in IF

Mouse Anti-NME1 Recombinant Antibody (CBT946) (V2LY-0625-LY3159) in IHC

Mouse Anti-NME1 Recombinant Antibody (CBT946) (V2LY-0625-LY3159) in WB

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT946

Application

WB, IHC-P, IF, ICC, FC, ELISA

Immunogen

Purified recombinant fragment of human NM23-H1 expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
FC	1:200-1:400
IF	1:200-1:1,000
IHC	1:200-1:1,000
WB	1:500-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Buffer

BSA, Glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

NME/NM23 Nucleoside Diphosphate Kinase 1

Entrez Gene ID

4830

UniProt ID

Q12879

Function

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. During GZMA-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.

Biological Process

Cell differentiationIEA:UniProtKB-KW
CTP biosynthetic processIEA:InterPro
EndocytosisIEA:UniProtKB-KW
GTP biosynthetic processIEA:InterPro
LactationIEA:Ensembl
Negative regulation of cell population proliferationManual Assertion Based On ExperimentTAS:UniProtKB
Nervous system developmentIEA:UniProtKB-KW
Positive regulation of DNA bindingManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of epithelial cell proliferationManual Assertion Based On ExperimentIMP:HGNC-UCL
Regulation of apoptotic processManual Assertion Based On ExperimentTAS:UniProtKB
UTP biosynthetic processIEA:InterPro

Cellular Location

Cytoplasm
Nucleus
Cell-cycle dependent nuclear localization which can be induced by interaction with Epstein-barr viral proteins or by degradation of the SET complex by GzmA.

Iuso, D., Garcia-Saez, I., Couté, Y., Yamaryo-Botté, Y., Boeri Erba, E., Adrait, A., ... & Khochbin, S. (2023). Nucleoside diphosphate kinases 1 and 2 regulate a protective liver response to a high-fat diet. Science Advances, 9(36), eadh0140.

Adam, K., Lesperance, J., Hunter, T., & Zage, P. E. (2020). The potential functional roles of NME1 histidine kinase activity in neuroblastoma pathogenesis. International journal of molecular sciences, 21(9), 3319.

Adam, K., Ning, J., Reina, J., & Hunter, T. (2020). NME/NM23/NDPK and histidine phosphorylation. International journal of molecular sciences, 21(16), 5848.

Mátyási, B., Farkas, Z., Kopper, L., Sebestyén, A., Boissan, M., Mehta, A., & Takács-Vellai, K. (2020). The function of NM23-H1/NME1 and its homologs in major processes linked to metastasis. Pathology & Oncology Research, 26, 49-61.

Radić, M., Šoštar, M., Weber, I., Ćetković, H., Slade, N., & Herak Bosnar, M. (2020). The subcellular localization and oligomerization preferences of NME1/NME2 upon radiation-induced DNA damage. International Journal of Molecular Sciences, 21(7), 2363.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

NME1 Matched Antibody Pair (803) (CAT#: APMAB-803LY)

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Isotype control

For research use only. Not intended for any clinical use.

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