Rabbit Anti-NPR2 Recombinant Antibody (
19C7) (V2LY-0725-LY1484)

Name: Rabbit Anti-NPR2 Recombinant Antibody (19C7)
SKU: V2LY-0725-LY1484
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-NPR2 Recombinant Antibody (19C7) (V2LY-0725-LY1484) in WB

Western Blot
Positive WB detected in: HEPG2 whole cell lysate, MCF7 whole cell lysate
All lanes: ANPRB antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 117 kDa
Observed band size: 117 kDa

Rabbit Anti-NPR2 Recombinant Antibody (19C7) (V2LY-0725-LY1484) in IF

Immunofluorescence staining of Hela with V2LY-0725-LY1484 at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 501-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Rabbit Anti-NPR2 Recombinant Antibody (19C7) (V2LY-0725-LY1484) in FC

Overlay Peak curve showing Hela cells surface stained with V2LY-0725-LY1484 (red line) at 1:50. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

19C7

Application

ELISA, WB, IF, FC

Immunogen

A synthesized peptide derived from Human NPR2.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IF	1:50-1:200
FC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

natriuretic peptide receptor B/guanylate cyclase B (atrionatriuretic peptide receptor B)

Entrez Gene ID

4882

UniProt ID

P20594

Function

Receptor for the C-type natriuretic peptide NPPC/CNP hormone. Has guanylate cyclase activity upon binding of its ligand. May play a role in the regulation of skeletal growth.

Biological Process

Bone developmentIEA:Ensembl
Cellular response to granulocyte macrophage colony-stimulating factor stimulusManual Assertion Based On ExperimentIEP:UniProtKB
cGMP biosynthetic processManual Assertion Based On ExperimentIDA:GO_Central
cGMP-mediated signalingIEA:Ensembl
Negative regulation of meiotic cell cycleIEA:Ensembl
Negative regulation of oocyte maturationIEA:Ensembl
OssificationIEA:UniProtKB-KW
Positive regulation of cGMP-mediated signalingIEA:Ensembl
Receptor guanylyl cyclase signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of blood pressureManual Assertion Based On ExperimentTAS:ProtInc
Reproductive processIEA:Ensembl
Signal transductionManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Cell membrane

Involvement in disease

Acromesomelic dysplasia 1 (AMD1):
A form of acromesomelic dysplasia, a skeletal disorder characterized by short stature, very short limbs and hand/foot malformations. The severity of limb abnormalities increases from proximal to distal with profoundly affected hands and feet showing brachydactyly and/or rudimentary fingers (knob-like fingers). AMD1 is an autosomal recessive form characterized by axial skeletal involvement with wedging of vertebral bodies. All skeletal elements are present but show abnormal rates of linear growth.
Epiphyseal chondrodysplasia, Miura type (ECDM):
An overgrowth syndrome characterized by tall stature, long hands and feet with arachnodactyly, macrodactyly of the great toes, scoliosis, coxa valga and slipped capital femoral epiphysis.
Short stature with non-specific skeletal abnormalities (SNSK):
A condition characterized by short stature, defined as a height less than 2 SD below normal, and no endocrine abnormalities.

Topology

Extracellular: 23-458
Helical: 459-478
Cytoplasmic: 479-1047

PTM

Phosphorylated (PubMed:26980729).
Phosphorylation of the protein kinase-like domain is required for full activation by CNP (By similarity).
Glycosylated.

Korshunov, V. A., Smolock, E. M., Wines‐Samuelson, M. E., Faiyaz, A., Mickelsen, D. M., Quinn, B., ... & Berk, B. C. (2020). Natriuretic peptide receptor 2 locus contributes to carotid remodeling. Journal of the American Heart Association, 9(10), e014257.

Dugbartey, G. J., Quinn, B., Luo, L., Mickelsen, D. M., Ture, S. K., Morrell, C. N., ... & Korshunov, V. A. (2019). The protective role of natriuretic peptide receptor 2 against high salt injury in the Renal Papilla. The American Journal of Pathology, 189(9), 1721-1731.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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