Rabbit Anti-NUDC Recombinant Antibody (
6C3) (V2LY-0725-LY1454)

Name: Rabbit Anti-NUDC Recombinant Antibody (6C3)
SKU: V2LY-0725-LY1454
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-NUDC Recombinant Antibody (6C3) (V2LY-0725-LY1454) in WB

Western Blot
Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, 293T whole cell lysate, A549 whole cell lysate, Rat Brain whole cell lysate, Mouse Brain whole cell lysate
All lanes: NUDC antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39 kDa
Observed band size: 45 kDa

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

6C3

Application

ELISA, WB

Immunogen

A synthesized peptide derived from human NUDC.

Host Species

Rabbit

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

nuclear distribution gene C homolog (A. nidulans)

Entrez Gene ID

10726

UniProt ID

Q9Y266

Function

Plays a role in neurogenesis and neuronal migration (By similarity).
Necessary for correct formation of mitotic spindles and chromosome separation during mitosis (PubMed:12852857, PubMed:12679384, PubMed:25789526).
Necessary for cytokinesis and cell proliferation (PubMed:12852857, PubMed:12679384).

Biological Process

Cell divisionIEA:UniProtKB-KW
Mitotic metaphase plate congressionManual Assertion Based On ExperimentIMP:UniProtKB
Mitotic spindle organizationManual Assertion Based On ExperimentIMP:UniProtKB
Nuclear migrationIEA:Ensembl
Protein foldingManual Assertion Based On ExperimentIBA:GO_Central
Response to peptide hormoneIEA:Ensembl

Cellular Location

Cytoplasm, cytoskeleton
Nucleus
Cytoplasm, cytoskeleton, spindle
Midbody
In a filamentous pattern adjacent to the nucleus of migrating cerebellar granule cells. Colocalizes with tubulin and dynein and with the microtubule organizing center. Distributed throughout the cytoplasm of non-migrating cells. A small proportion is nuclear, in a punctate pattern. Localizes to the mitotic spindle in a EML4-dependent manner (PubMed:25789526).

PTM

Reversibly phosphorylated on serine residues during the M phase of the cell cycle. Phosphorylation on Ser-274 and Ser-326 is necessary for correct formation of mitotic spindles and chromosome separation during mitosis. Phosphorylated by PLK and other kinases.

Vassileva, V., Georgieva, M., Todorov, D., & Mishev, K. (2023). Small Sized Yet Powerful: Nuclear Distribution C Proteins in Plants. Plants, 13(1), 119.

Garner, M. A., Hubbard, M. G., Boitet, E. R., Hubbard, S. T., Gade, A., Ying, G., ... & Gross, A. K. (2023). NUDC is critical for rod photoreceptor function, maintenance, and survival. bioRxiv, 2023-11.

Levi, H. J., Hubbard, M., Garner, M. A., Hollingsworth, T. J., Jiang, K., Nelson, N., ... & Gross, A. K. (2022). Dynein Dysregulation Due to the Absence of NUDC leads to Mitochondrial Mislocalization and Dysfunction in Rod Photoreceptors. Investigative Ophthalmology & Visual Science, 63(7), 3878-A0080.

Kawano, D., Pinter, K., Chlebowski, M., Petralia, R. S., Wang, Y. X., Nechiporuk, A. V., & Drerup, C. M. (2022). NudC regulated Lis1 stability is essential for the maintenance of dynamic microtubule ends in axon terminals. Iscience, 25(10).

Islam, M. A., Choi, H. J., Dash, R., Sharif, S. R., Oktaviani, D. F., Seog, D. H., & Moon, I. S. (2020). N-acetyl-D-Glucosamine kinase interacts with NudC and Lis1 in dynein motor complex and promotes cell migration. International Journal of Molecular Sciences, 22(1), 129.

Gross, A. K., Boitet, E. R., Reish, N. J., Benefield, D., Ying, G., Baehr, W., ... & Hubbard, M. (2020). NUDC is critical for regulation of photoreceptor cytoskeletal network. Investigative Ophthalmology & Visual Science, 61(7), 1121-1121.

Boitet, E. R., Reish, N. J., Hubbard, M. G., & Gross, A. K. (2019). NudC regulates photoreceptor disk morphogenesis and rhodopsin localization. The FASEB Journal, 33(8), 8799.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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