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Mouse Anti-PLK3 Recombinant Antibody (B37-2) (CBMAB-P2148-YC)

Provided herein is a Mouse monoclonal antibody against Human Polo Like Kinase 3. The antibody can be used for immunoassay techniques, such as WB, IP.
See all PLK3 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
B37-2
Antibody Isotype
IgG1
Application
WB, IP

Basic Information

Immunogen
Recombinant human PRK Protein, AA 334-607
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Polo like kinase 3
Introduction
PLK3 is a member of the highly conserved polo-like kinase family of serine/threonine kinases. Members of this family are characterized by an amino-terminal kinase domain and a carboxy-terminal bipartite polo box domain that functions as a substrate-binding motif and a cellular localization signal. Polo-like kinases are important regulators of cell cycle progression. This gene has also been implicated in stress responses and double-strand break repair.
Entrez Gene ID
1263
UniProt ID
Q9H4B4
Alternative Names
CNK; FNK; PLK-3; PRK
Function
Serine/threonine-protein kinase involved in cell cycle regulation, response to stress and Golgi disassembly. Polo-like kinases act by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates ATF2, BCL2L1, CDC25A, CDC25C, CHEK2, HIF1A, JUN, p53/TP53, p73/TP73, PTEN, TOP2A and VRK1. Involved in cell cycle regulation: required for entry into S phase and cytokinesis. Phosphorylates BCL2L1, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis. Plays a key role in response to stress: rapidly activated upon stress stimulation, such as ionizing radiation, reactive oxygen species (ROS), hyperosmotic stress, UV irradiation and hypoxia. Involved in DNA damage response and G1/S transition checkpoint by phosphorylating CDC25A, p53/TP53 and p73/TP73. Phosphorylates p53/TP53 in response to reactive oxygen species (ROS), thereby promoting p53/TP53-mediated apoptosis. Phosphorylates CHEK2 in response to DNA damage, promoting the G2/M transition checkpoint. Phosphorylates the transcription factor p73/TP73 in response to DNA damage, leading to inhibit p73/TP73-mediated transcriptional activation and pro-apoptotic functions. Phosphorylates HIF1A and JUN is response to hypoxia. Phosphorylates ATF2 following hyperosmotic stress in corneal epithelium. Also involved in Golgi disassembly during the cell cycle: part of a MEK1/MAP2K1-dependent pathway that induces Golgi fragmentation during mitosis by mediating phosphorylation of VRK1. May participate in endomitotic cell cycle, a form of mitosis in which both karyokinesis and cytokinesis are interrupted and is a hallmark of megakaryocyte differentiation, via its interaction with CIB1.
Biological Process
Apoptotic processManual Assertion Based On ExperimentTAS:UniProtKB
Cellular response to DNA damage stimulusManual Assertion Based On ExperimentIDA:UniProtKB
Cytoplasmic microtubule organizationManual Assertion Based On ExperimentIMP:UniProtKB
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrestTAS:Reactome
Endomitotic cell cycleManual Assertion Based On ExperimentTAS:UniProtKB
G1/S transition of mitotic cell cycleManual Assertion Based On ExperimentIMP:UniProtKB
G2/M transition of mitotic cell cycleManual Assertion Based On ExperimentTAS:UniProtKB
Golgi disassemblyManual Assertion Based On ExperimentIDA:UniProtKB
Mitotic cell cycleManual Assertion Based On ExperimentIBA:GO_Central
Mitotic G1/S transition checkpoint signalingISS:UniProtKB
Negative regulation of apoptotic processManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of chaperone-mediated autophagyManual Assertion Based On ExperimentIDA:ParkinsonsUK-UCL
Positive regulation of intracellular protein transportManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of proteasomal ubiquitin-dependent protein catabolic process involved in cellular response to hypoxiaManual Assertion Based On ExperimentIDA:UniProtKB
Protein kinase B signalingISS:UniProtKB
Protein phosphorylationManual Assertion Based On ExperimentTAS:ProtInc
Regulation of cell divisionManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of cytokinesisManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of signal transduction by p53 class mediatorTAS:Reactome
Response to osmotic stressManual Assertion Based On ExperimentIDA:UniProtKB
Response to radiationManual Assertion Based On ExperimentIDA:UniProtKB
Response to reactive oxygen speciesManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Cytoplasm
Nucleus
Nucleus, nucleolus
Golgi apparatus
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Translocates to the nucleus upon cisplatin treatment. Localizes to the Golgi apparatus during interphase. According to a report, PLK3 localizes only in the nucleolus and not in the centrosome, or in any other location in the cytoplasm (PubMed:17264206).
The discrepancies in results may be explained by the PLK3 antibody specificity, by cell line-specific expression or post-translational modifications.
PTM
Phosphorylated in an ATM-dependent manner following DNA damage. Phosphorylated as cells enter mitosis and dephosphorylated as cells exit mitosis.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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