PLK3

PLK3 (Polo Like Kinase 3) is a protein coding gene. Among its related pathways are Gene Expression and Integrated Breast Cancer Pathway. Gene Ontology annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of the gene is PLK2.

Polo like kinase 3

Serine/threonine-protein kinase involved in cell cycle regulation, response to stress and Golgi disassembly. Polo-like kinases act by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates ATF2, BCL2L1, CDC25A, CDC25C, CHEK2, HIF1A, JUN, p53/TP53, p73/TP73, PTEN, TOP2A and VRK1. Involved in cell cycle regulation: required for entry into S phase and cytokinesis. Phosphorylates BCL2L1, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis. Plays a key role in response to stress: rapidly activated upon stress stimulation, such as ionizing radiation, reactive oxygen species (ROS), hyperosmotic stress, UV irradiation and hypoxia. Involved in DNA damage response and G1/S transition checkpoint by phosphorylating CDC25A, p53/TP53 and p73/TP73. Phosphorylates p53/TP53 in response to reactive oxygen species (ROS), thereby promoting p53/TP53-mediated apoptosis. Phosphorylates CHEK2 in response to DNA damage, promoting the G2/M transition checkpoint. Phosphorylates the transcription factor p73/TP73 in response to DNA damage, leading to inhibit p73/TP73-mediated transcriptional activation and pro-apoptotic functions. Phosphorylates HIF1A and JUN is response to hypoxia. Phosphorylates ATF2 following hyperosmotic stress in corneal epithelium. Also involved in Golgi disassembly during the cell cycle: part of a MEK1/MAP2K1-dependent pathway that induces Golgi fragmentation during mitosis by mediating phosphorylation of VRK1. May participate in endomitotic cell cycle, a form of mitosis in which both karyokinesis and cytokinesis are interrupted and is a hallmark of megakaryocyte differentiation, via its interaction with CIB1.

Apoptotic processManual Assertion Based On ExperimentTAS:UniProtKB
Cellular response to DNA damage stimulusManual Assertion Based On ExperimentIDA:UniProtKB
Cytoplasmic microtubule organizationManual Assertion Based On ExperimentIMP:UniProtKB
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrestTAS:Reactome
Endomitotic cell cycleManual Assertion Based On ExperimentTAS:UniProtKB
G1/S transition of mitotic cell cycleManual Assertion Based On ExperimentIMP:UniProtKB
G2/M transition of mitotic cell cycleManual Assertion Based On ExperimentTAS:UniProtKB
Golgi disassemblyManual Assertion Based On ExperimentIDA:UniProtKB
Mitotic cell cycleManual Assertion Based On ExperimentIBA:GO_Central
Mitotic G1/S transition checkpoint signalingISS:UniProtKB
Negative regulation of apoptotic processManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of chaperone-mediated autophagyManual Assertion Based On ExperimentIDA:ParkinsonsUK-UCL
Positive regulation of intracellular protein transportManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of proteasomal ubiquitin-dependent protein catabolic process involved in cellular response to hypoxiaManual Assertion Based On ExperimentIDA:UniProtKB
Protein kinase B signalingISS:UniProtKB
Protein phosphorylationManual Assertion Based On ExperimentTAS:ProtInc
Regulation of cell divisionManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of cytokinesisManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of signal transduction by p53 class mediatorTAS:Reactome
Response to osmotic stressManual Assertion Based On ExperimentIDA:UniProtKB
Response to radiationManual Assertion Based On ExperimentIDA:UniProtKB
Response to reactive oxygen speciesManual Assertion Based On ExperimentIDA:UniProtKB

Cytoplasm
Nucleus
Nucleus, nucleolus
Golgi apparatus
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Translocates to the nucleus upon cisplatin treatment. Localizes to the Golgi apparatus during interphase. According to a report, PLK3 localizes only in the nucleolus and not in the centrosome, or in any other location in the cytoplasm (PubMed:17264206).
The discrepancies in results may be explained by the PLK3 antibody specificity, by cell line-specific expression or post-translational modifications.

Phosphorylated in an ATM-dependent manner following DNA damage. Phosphorylated as cells enter mitosis and dephosphorylated as cells exit mitosis.