Mouse Anti-RHD Recombinant Antibody (
28D12) (V2LY-0725-LY872)

Name: Mouse Anti-RHD Recombinant Antibody (28D12)
SKU: V2LY-0725-LY872
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-RHD Recombinant Antibody (28D12) (V2LY-0725-LY872) in FC

Overlay Peak curve showing Hela cells surface stained with V2LY-0725-LY872 (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of>10, 000 events was performed.

Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

28D12

Application

ELISA, FC

Immunogen

Recombinant Human RHD protein.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
FC	1:20-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, Glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Rh Blood Group D Antigen

Entrez Gene ID

6007

UniProt ID

Q02161

Function

May be part of an oligomeric complex which is likely to have a transport or channel function in the erythrocyte membrane.

Biological Process

Biological Process ammonium homeostasisManual Assertion Based On ExperimentIBA:GO_Central
Biological Process ammonium transmembrane transportManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Cell membrane

Involvement in disease

Hemolytic disease of fetus and newborn, RH-induced (HDFNRH):
A disease that occurs in pregnancies in which mothers who lack the D antigen (RhD) of the Rh blood group have been exposed to the RhD-positive red cells of the fetus. The resulting maternal autoantibodies cross the placenta and destroy fetal red cells.

PTM

Palmitoylated.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-RHD Recombinant Antibody (3H2011) (CAT#: CBMAB-R2507-CN)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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