Sign in or Register   Sign in or Register
  |  

Mouse Anti-TOPORS Recombinant Antibody (CBYJT-4223) (CBMAB-T3687-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to TOPORS (TOP1 Binding Arginine/Serine Rich Protein). The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all TOPORS antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYJT-4223
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Immunogen
TOPORS (NP_005793, 98 a.a. ~ 205 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.Immunogen sequence: SPDSKCPICL DRFDNVSYLD RCLHKFCFRC VQEWSKNKAE CPLCKQPFDS IFHSVRAEDD FKEYVLRPSY NGSFVTPDRR FRYRTTLTRE RNASVYSPSG PVNRRTTT
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 98-205

Target

Full Name
topoisomerase I binding, arginine/serine-rich
Introduction
TOPORS is a nuclear protein which is serine and arginine rich, and contains a RING-type zinc finger domain. TOPORS is highly expressed in the testis, and plays a role as an ubiquitin-protein E3 ligase. Mutations in TOPORS are associated with retinitis pigmentosa type 31.
Entrez Gene ID
10210
UniProt ID
Q9NS56
Alternative Names
TOP1 Binding Arginine/Serine Rich Protein; Topoisomerase I Binding, Arginine/Serine-Rich, E3 Ubiquitin Protein Ligase; Topoisomerase I-Binding RING Finger Protein; RING-Type E3 Ubiquitin Transferase Topors; Tumor Suppressor P53-Binding Protein 3; SUMO1-Protein E3 Ligase Topors; TP53BPL; P53BP3
Function
Functions as an E3 ubiquitin-protein ligase and as an E3 SUMO1-protein ligase. Probable tumor suppressor involved in cell growth, cell proliferation and apoptosis that regulates p53/TP53 stability through ubiquitin-dependent degradation. May regulate chromatin modification through sumoylation of several chromatin modification-associated proteins. May be involved in DNA damage-induced cell death through IKBKE sumoylation.
Biological Process
Biological Process cellular response to DNA damage stimulus Source:UniProtKB1 Publication
Biological Process DNA-templated transcription Source:UniProtKB1 Publication
Biological Process intrinsic apoptotic signaling pathway in response to DNA damage Source:UniProtKB1 Publication
Biological Process intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source:Ensembl
Biological Process maintenance of protein location in nucleus Source:UniProtKB1 Publication
Biological Process negative regulation of apoptotic process Source:BHF-UCLBy Similarity
Biological Process photoreceptor cell outer segment organization Source:BHF-UCLBy Similarity
Biological Process positive regulation of DNA-templated transcription Source:Ensembl
Biological Process positive regulation of ubiquitin-protein transferase activity Source:BHF-UCL1 Publication
Biological Process proteasome-mediated ubiquitin-dependent protein catabolic process Source:UniProtKB1 Publication
Biological Process protein K48-linked ubiquitination Source:BHF-UCL1 Publication
Biological Process protein localization to nucleus Source:UniProtKB1 Publication
Biological Process protein monoubiquitination Source:BHF-UCL1 Publication
Biological Process protein polyubiquitination Source:BHF-UCL1 Publication
Biological Process protein sumoylation Source:UniProtKB1 Publication
Biological Process regulation of cell population proliferation Source:Ensembl
Biological Process retina layer formation Source:BHF-UCLBy Similarity
Biological Process retinal cone cell development Source:BHF-UCLBy Similarity
Biological Process retinal rod cell development Source:BHF-UCLBy Similarity
Biological Process ubiquitin-dependent protein catabolic process Source:UniProtKB1 Publication
Cellular Location
Nucleus
Nucleus, PML body
Localizes to discrete nuclear foci which partly overlap with PML nuclear bodies. Targeted to PML nuclear bodies upon DNA damage.
Involvement in disease
Retinitis pigmentosa 31 (RP31):
A retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
PTM
Phosphorylation at Ser-98 regulates the E3 ubiquitin-protein ligase activity but not the SUMO1-protein ligase activity. Phosphorylation at Ser-718 increases the E3 ubiquitin-protein ligase activity versus the SUMO1-protein ligase activity resulting in increased p53/TP53 ubiquitination and degradation.
Sumoylated.
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-TOPORS Recombinant Antibody (CBYJT-4223)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare