Sign in or Register   Sign in or Register
  |  

Mouse Anti-WAS Recombinant Antibody (CBWJW-047) (CBMAB-W0201-WJ)

This product is a mouse antibody that recognizes WAS. The antibody CBWJW-047 can be used for immunoassay techniques such as: ELISA, WB.
See all WAS antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBWJW-047
Antibody Isotype
IgG2b, Κ
Application
ELISA, WB

Basic Information

Immunogen
WAS (NP_000368, aa57-170) partial recombinant protein with GST tag. MW of the GST tag alone is 26 Kda.
Specificity
Human
Antibody Isotype
IgG2b, Κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Wiskott-Aldrich syndrome (eczema-thrombocytopenia)
Introduction
WAS is a protein-coding gene. The WAS family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The WAS gene product is a cytoplasmic protein, expressed exclusively in hematopoietic cells, which show signalling and cytoskeletal abnormalities in WAS patients. Diseases associated with WAS include Thrombocytopenia 1 and Neutropenia, Severe Congenital, X-Linked. An important paralog of this gene is WASL.
Entrez Gene ID
7454
UniProt ID
P42768
Alternative Names
THC; IMD2; SCNX; THC1; WASP; WASPA
Function
Effector protein for Rho-type GTPases that regulates actin filament reorganization via its interaction with the Arp2/3 complex (PubMed:12235133, PubMed:12769847, PubMed:16275905).
Important for efficient actin polymerization (PubMed:8625410, PubMed:12235133, PubMed:16275905).
Possible regulator of lymphocyte and platelet function (PubMed:9405671).
Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria (PubMed:18650809).
In addition to its role in the cytoplasmic cytoskeleton, also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed:20574068).
Promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed:29925947).
Biological Process
Biological Process actin filament polymerization Source:UniProtKB1 Publication
Biological Process actin filament-based movement Source:GO_Central1 Publication
Biological Process actin polymerization or depolymerization Source:ProtInc1 Publication
Biological Process blood coagulation Source:ProtInc1 Publication
Biological Process Cdc42 protein signal transduction Source:CAFA1 Publication
Biological Process cellular response to type II interferon Source:Ensembl
Biological Process defense response Source:ProtInc1 Publication
Biological Process endosomal transport Source:Ensembl
Biological Process epidermis development Source:ProtInc1 Publication
Biological Process immune response Source:HGNC-UCL1 Publication
Biological Process negative regulation of cell motility Source:CACAO1 Publication
Biological Process negative regulation of stress fiber assembly Source:CAFA1 Publication
Biological Process positive regulation of Arp2/3 complex-mediated actin nucleation Source:InterPro
Biological Process positive regulation of double-strand break repair via homologous recombination Source:UniProtKB1 Publication
Biological Process positive regulation of transcription by RNA polymerase II Source:UniProtKB1 Publication
Biological Process protein-containing complex assembly Source:ProtInc1 Publication
Biological Process regulation of actin polymerization or depolymerization Source:CAFA1 Publication
Biological Process regulation of lamellipodium assembly Source:CAFA2 Publications
Biological Process regulation of stress fiber assembly Source:CAFA2 Publications
Biological Process regulation of T cell antigen processing and presentation Source:CACAO1 Publication
Biological Process T cell activation Source:Ensembl
Cellular Location
Cytoplasm, cytoskeleton
Nucleus
Involvement in disease
Wiskott-Aldrich syndrome (WAS):
An X-linked recessive immunodeficiency characterized by eczema, thrombocytopenia, recurrent infections, and bloody diarrhea. Death usually occurs before age 10.
Thrombocytopenia 1 (THC1):
A form of thrombocytopenia, a hematologic disorder defined by a decrease in the number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting.
Neutropenia, severe congenital, X-linked (XLN):
A disorder of hematopoiesis characterized by maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections.
PTM
Phosphorylated at Tyr-291 by FYN and HCK, inducing WAS effector activity after TCR engagement. Phosphorylation at Tyr-291 enhances WAS activity in promoting actin polymerization and filopodia formation.
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-WAS Recombinant Antibody (CBWJW-047)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare