Human Recombinant WTAP protein, GST Tag-1 (V2LY-0526-LY7679)

Name: Human Recombinant WTAP protein, GST Tag-1
SKU: V2LY-0526-LY7679
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant WTAP protein, GST Tag consist of Amino Acid: 1-151 and predicts a molecular mass of 45 kDa.

Molecule Mass

45 kDa

Sequence

Amino Acid: 1-151

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>70% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

WTAP

Function

Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (PubMed:29507755).
Required for accumulation of METTL3 and METTL14 to nuclear speckle (PubMed:24316715, PubMed:24407421, PubMed:24981863).
Acts as a mRNA splicing regulator (PubMed:12444081).
Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability (PubMed:17088532).
Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes (PubMed:17095724).

Biological Process

Biological Process cell cycle Source:UniProtKB-KW
Biological Process mRNA methylation Source:UniProtKB3 Publications
Biological Process mRNA processing Source:UniProtKB-KW
Biological Process regulation of alternative mRNA splicing, via spliceosome Source:UniProtKB1 Publication
Biological Process RNA splicing Source:UniProtKB-KW

Cellular Location

Nucleus speckle
Nucleus, nucleoplasm
Cytoplasm
Mainly nuclear with some fraction located in the cytoplasm. ZC3H13 is required to anchor component of the MACOM subcomplex, such as VIRMA, in the nucleus.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant WTAP protein, GST Tag (CAT#: V2LY-0526-LY7678)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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