ADNP Antibodies

Structure of ADNP

ADNP is a relatively large multifunctional protein with a molecular weight of approximately 140 kDa. This protein is composed of 1,102 amino acids, and its structural features are mainly reflected in multiple key functional domains.

The core functional region of this protein consists of two tandem PhD-type zinc finger domains, which can directly bind to the histone H3 tail and mediate the interaction with the chromatin remodeling complex. Its C-terminal contains a nuclear localization signal peptide, while the N-terminal region is responsible for binding to various partner proteins. The entire molecule forms a rigid spatial conformation through nine conserved zinc finger modules, which is crucial for its function as a transcriptional regulatory factor.

Fig. 1 3D structure of ADNP retrieved from Alphafold database.¹

Key structural properties of ADNP:

• Contains nine highly conserved zinc-finger domains
• There is a mechanism of nuclear transfer mediated by nuclear localization signal
• Involved in transcriptional regulation through homeotypic box domains
• Form a stable β -folded lamellar and α -helical combined structure

Functions of ADNP

The core function of ADNP protein is to regulate neural development and maintain cellular homeostasis, and its mechanism of action covers multiple levels:

This protein functions in the form of a complex regulatory network through the nine zinc finger domains and nuclear localization signals it contains. The loss of its function has a much greater impact on the central nervous system than a defect in a single pathway, demonstrating its irreplaceable core position in the nervous system.

Applications of ADNP and ADNP Antibody in Literature

1. Sun X, Peng X, et al. "ADNP promotes neural differentiation by modulating Wnt/β-catenin signaling." Nature communications 11.1 (2020): 2984. https://doi.org/10.1038/s41467-020-16799-0

The article indicates that ADNP stabilizes β-Catenin and enhances the Wnt signaling pathway by binding to β-Catenin and preventing it from binding to the Axin/APC degradation complex, thereby promoting neural induction and differentiation. The absence of ADNP can lead to the degradation of β-Catenin, causing neurodevelopmental abnormalities, which provides new insights into the pathological mechanism of ADNP syndrome.

2. Gozes, Illana. "The ADNP syndrome and CP201 (NAP) potential and hope." Frontiers in neurology 11 (2020): 608444. https://doi.org/10.3389/fneur.2020.608444

The article indicates that ADNP syndrome is a rare autism spectrum disorder caused by de novo mutations in the ADNP gene. This mutation leads to neurological dysfunction, manifested as intellectual, social and motor disorders. Studies have shown that its active fragment CP201 can alleviate related symptoms in preclinical models, and clinical trials are planned to be conducted.

3. D’Incal, Claudio Peter, et al. "Chromatin remodeler Activity-Dependent Neuroprotective Protein (ADNP) contributes to syndromic autism." Clinical Epigenetics 15.1 (2023): 45. https://doi.org/10.1186/s13148-023-01450-8

The article indicates that the ADNP gene is one of the most common mutant genes causing Helsmoortel-Van der Aa syndrome (a syndromic type of autism). The proteins encoded by it mainly function by regulating chromatin remodeling. Abnormal ADNP function can trigger pleiotropic clinical features, which provides a mechanism-level explanation for understanding the complex manifestations of this syndrome.

4. Ferreira, Ana CF, et al. "Neuroprotective protein ADNP-dependent histone remodeling complex promotes T helper 2 immune cell differentiation." Immunity 56.7 (2023): 1468-1484. https://doi.org/10.1016/j.immuni.2023.05.010

This study first discovered that ADNP is indispensable in the differentiation of Th2 cells and allergic immune responses. The mechanism lies in that ADNP can act as a key bridge, by recruiting chromatin remodeling complexes (such as CHD4 and BRG1), to activate the chromatin accessibility of type 2 cytokine gene loci, thereby driving the specialization of immune cells.

5. Maugeri, Grazia, et al. "Activity-dependent neuroprotective protein (ADNP): An overview of its role in the eye." International Journal of Molecular Sciences 23.21 (2022): 13654. https://doi.org/10.3390/ijms232113654

This study indicates that ADNP and its derivative NAP are widely expressed in ocular tissues such as the retina and cornea. Studies have shown that NAP possesses neuroprotective, antioxidant and anti-apoptotic properties, and has demonstrated good safety in clinical trials. It is expected to become a potential candidate drug for the treatment of various eye diseases.

Creative Biolabs: ADNP Antibodies for Research

Creative Biolabs specializes in the production of high-quality ADNP antibodies for research and industrial applications. Our portfolio includes monoclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

• Custom ADNP Antibody Development: Tailor-made solutions to meet specific research requirements.
• Bulk Production: Large-scale antibody manufacturing for industry partners.
• Technical Support: Expert consultation for protocol optimization and troubleshooting.
• Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our ADNP antibodies, custom preparations, or technical support, contact us at email.