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Mouse Anti-AFAP1 Recombinant Antibody (V2-180040) (CBMAB-A1447-YC)

Provided herein is a Mouse monoclonal antibody against Human Actin Filament Associated Protein 1. The antibody can be used for immunoassay techniques, such as FC, ELISA.
See all AFAP1 antibodies
Published Data
Mouse Anti-AFAP1 Recombinant Antibody (16F50) (CBMAB-A1447-YC) in FC
Representative scatterplot for tumor cells stained with CellROX and PE-conjugated FAP antibody. Only VybrantDiO-labeled FIBs were gated for analysis of ROS and FAP expression. The percentage of cells in each quadrant is indicated. The numbers in brackets on the Y axis indicate CellROX signal intensity. ...View More
Chan, J. S. K., Tan, M. J., Sng, M. K., Teo, Z., Phua, T., Choo, C. C., ... & Tan, N. S. (2018). Cancer-associated fibroblasts enact field cancerization by promoting extratumoral oxidative stress. Cell death & disease, 8(1), e2562-e2562.

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-180040
Antibody Isotype
IgG1
Application
IHC-F, ELISA

Basic Information

Immunogen
Purified recombinant aFAP
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
ELISA1:1,000-1:2,000
FC1:10-1:20

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS, Trehalose
Preservative
None
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Actin Filament Associated Protein 1
Introduction
AFAP1 is a Src binding partner. It may represent a potential modulator of actin filament integrity in response to cellular signals, and may function as an adaptor protein by linking Src family members and/or other signaling proteins to actin filaments.
Entrez Gene ID
60312
UniProt ID
Q8N556
Alternative Names
Actin Filament Associated Protein 1; 110 KDa Actin Filament-Associated Protein; AFAP-110; AFAP; Actin Filament-Associated Protein, 110 KDa; Actin Filament-Associated Protein 1; AFAP110;
Function
Can cross-link actin filaments into both network and bundle structures (By similarity). May modulate changes in actin filament integrity and induce lamellipodia formation. May function as an adapter molecule that links other proteins, such as SRC and PKC to the actin cytoskeleton. Seems to play a role in the development and progression of prostate adenocarcinoma by regulating cell-matrix adhesions and migration in the cancer cells.
Biological Process
Regulation of cytoskeleton organization
Regulation of signal transduction
Cellular Location
Stress fiber
PTM
Phosphorylated on tyrosine residues by SRC.

Fan, H., Dong, Q., Yu, J., Yin, L., Sun, X., Yang, X., & Zhang, W. (2021). Knockdown of long non-coding RNA actin filament-associated protein 1 antisense RNA1 (AFAP1-AS1) inhibits epithelial-mesenchymal transition, invasion and migration of TPC-1 papillary thyroid cancer cells. Xi bao yu fen zi Mian yi xue za zhi= Chinese Journal of Cellular and Molecular Immunology, 37(1), 54-60.

Piacsek, M., Sand, A., Rovin, R. A., Bosenko, D., Konduri, S. D., Tjoe, J. A., & Yin, J. (2019). Transglutaminase 2 (TGM2) overexpression contributes to triple-negative breast cancer metastasis through AFAP1-dependent pathway.

Yu, S., Yang, D., Ye, Y., Liu, P., Chen, Z., Lei, T., ... & Wang, Z. (2019). Long noncoding RNA actin filament‐associated protein 1 antisense RNA 1 promotes malignant phenotype through binding with lysine‐specific demethylase 1 and repressing HMG box‐containing protein 1 in non‐small‐cell lung cancer. Cancer science, 110(7), 2211-2225.

Zhang, F., Li, J., Xiao, H., Zou, Y., Liu, Y., & Huang, W. (2018). AFAP1‐AS1: A novel oncogenic long non‐coding RNA in human cancers. Cell proliferation, 51(1), e12397.

Leng, X., Ding, X., Wang, S., Fang, T., Shen, W., Xia, W., ... & Yin, R. (2018). Long noncoding RNA AFAP1‑AS1 is upregulated in NSCLC and associated with lymph node metastasis and poor prognosis. Oncology letters, 16(1), 727-732.

Wang, M., Han, X., Sun, W., Li, X., Jing, G., & Zhang, X. (2018). Actin filament-associated protein 1-like 1 mediates proliferation and survival in non-small cell lung cancer cells. Medical science monitor: international medical journal of experimental and clinical research, 24, 215.

Tang, Y., He, Y., Shi, L., Yang, L., Wang, J., Lian, Y., ... & Zeng, Z. (2017). Co-expression of AFAP1-AS1 and PD-1 predicts poor prognosis in nasopharyngeal carcinoma. Oncotarget, 8(24), 39001.

Hoshi, Y., Uchida, Y., Tachikawa, M., Ohtsuki, S., & Terasaki, T. (2017). Actin filament‐associated protein 1 (AFAP‐1) is a key mediator in inflammatory signaling‐induced rapid attenuation of intrinsic P‐gp function in human brain capillary endothelial cells. Journal of neurochemistry, 141(2), 247-262.

Shi, X., Zhang, H., Wang, M., Xu, X., Zhao, Y., He, R., ... & Qin, R. (2017). LncRNA AFAP1-AS1 promotes growth and metastasis of cholangiocarcinoma cells. Oncotarget, 8(35), 58394.

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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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