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Mouse Anti-AFAP1 Recombinant Antibody (V2-611022) (CBMAB-A0175-LY)

The product is antibody recognizes AFAP. The antibody 3B12 immunoassay techniques such as: ELISA.
See all AFAP1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-611022
Antibody Isotype
IgG2a, κ
Application
ELISA

Basic Information

Immunogen
AFAP (NP_067651, 458 a.a. ~ 558 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH7.4
Preservative
None
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Actin Filament Associated Protein 1
Introduction
The protein encoded by this gene is a Src binding partner. It may represent a potential modulator of actin filament integrity in response to cellular signals, and may function as an adaptor protein by linking Src family members and/or other signaling proteins to actin filaments. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Entrez Gene ID
60312
UniProt ID
Q8N556
Alternative Names
AFAP; AFAP-110; FLJ56849
Function
Can cross-link actin filaments into both network and bundle structures (By similarity). May modulate changes in actin filament integrity and induce lamellipodia formation. May function as an adapter molecule that links other proteins, such as SRC and PKC to the actin cytoskeleton. Seems to play a role in the development and progression of prostate adenocarcinoma by regulating cell-matrix adhesions and migration in the cancer cells.
Biological Process
Regulation of cytoskeleton organization
Regulation of signal transduction
Cellular Location
Stress fiber
PTM
Phosphorylated on tyrosine residues by SRC.

Fan, H., Dong, Q., Yu, J., Yin, L., Sun, X., Yang, X., & Zhang, W. (2021). Knockdown of long non-coding RNA actin filament-associated protein 1 antisense RNA1 (AFAP1-AS1) inhibits epithelial-mesenchymal transition, invasion and migration of TPC-1 papillary thyroid cancer cells. Xi bao yu fen zi Mian yi xue za zhi= Chinese Journal of Cellular and Molecular Immunology, 37(1), 54-60.

Piacsek, M., Sand, A., Rovin, R. A., Bosenko, D., Konduri, S. D., Tjoe, J. A., & Yin, J. (2019). Transglutaminase 2 (TGM2) overexpression contributes to triple-negative breast cancer metastasis through AFAP1-dependent pathway.

Yu, S., Yang, D., Ye, Y., Liu, P., Chen, Z., Lei, T., ... & Wang, Z. (2019). Long noncoding RNA actin filament‐associated protein 1 antisense RNA 1 promotes malignant phenotype through binding with lysine‐specific demethylase 1 and repressing HMG box‐containing protein 1 in non‐small‐cell lung cancer. Cancer science, 110(7), 2211-2225.

Zhang, F., Li, J., Xiao, H., Zou, Y., Liu, Y., & Huang, W. (2018). AFAP1‐AS1: A novel oncogenic long non‐coding RNA in human cancers. Cell proliferation, 51(1), e12397.

Leng, X., Ding, X., Wang, S., Fang, T., Shen, W., Xia, W., ... & Yin, R. (2018). Long noncoding RNA AFAP1‑AS1 is upregulated in NSCLC and associated with lymph node metastasis and poor prognosis. Oncology letters, 16(1), 727-732.

Wang, M., Han, X., Sun, W., Li, X., Jing, G., & Zhang, X. (2018). Actin filament-associated protein 1-like 1 mediates proliferation and survival in non-small cell lung cancer cells. Medical science monitor: international medical journal of experimental and clinical research, 24, 215.

Tang, Y., He, Y., Shi, L., Yang, L., Wang, J., Lian, Y., ... & Zeng, Z. (2017). Co-expression of AFAP1-AS1 and PD-1 predicts poor prognosis in nasopharyngeal carcinoma. Oncotarget, 8(24), 39001.

Hoshi, Y., Uchida, Y., Tachikawa, M., Ohtsuki, S., & Terasaki, T. (2017). Actin filament‐associated protein 1 (AFAP‐1) is a key mediator in inflammatory signaling‐induced rapid attenuation of intrinsic P‐gp function in human brain capillary endothelial cells. Journal of neurochemistry, 141(2), 247-262.

Shi, X., Zhang, H., Wang, M., Xu, X., Zhao, Y., He, R., ... & Qin, R. (2017). LncRNA AFAP1-AS1 promotes growth and metastasis of cholangiocarcinoma cells. Oncotarget, 8(35), 58394.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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