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Rat Anti-ALCAM Recombinant Antibody (200622) (CBMAB-C4338-LY)

This product is antibody recognizes ALCAM. The antibody 200622 immunoassay techniques such as: IHC-F, WB.
See all ALCAM antibodies
Published Data
Rat Anti-ALCAM Recombinant Antibody (200622) in IF
Co-immunostaining of Cbln1 with Lhx2 or Alcam in spinal cord cross-sections during E10.5 showed expression of Cbln1 in the DCN neurons (white arrowheads) and FP (arrows). ...View More
Han, P., She, Y., Yang, Z., Zhuang, M., Wang, Q., Luo, X., ... & Ji, S. J. (2022). Cbln1 regulates axon growth and guidance in multiple neural regions. PLoS Biology, 20(11), e3001853.

Summary

Host Animal
Rat
Specificity
Mouse
Clone
200622
Antibody Isotype
IgG2a
Application
FC, WB

Basic Information

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse ALCAM/CD166 Trp28-Lys527
Host Species
Rat
Specificity
Mouse
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1 µg/ml
FC2.5 µg/10^6 cells

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS, Trehalose
Preservative
None
Concentration
0.5 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Activated Leukocyte Cell Adhesion Molecule
Introduction
This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2011]
Entrez Gene ID
11658
UniProt ID
Q61490
Function
Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts (PubMed:7760007, PubMed:15496415, PubMed:15048703, PubMed:16352806, PubMed:23169771, PubMed:24945728). Promotes T-cell activation and proliferation via its interactions with CD6 (PubMed:15048703, PubMed:16352806, PubMed:24945728). Contributes to the formation and maturation of the immunological synapse via its interactions with CD6 (PubMed:15294938, PubMed:16352806). Mediates homotypic interactions with cells that express ALCAM (PubMed:15496415, PubMed:16352806). Required for normal hematopoietic stem cell engraftment in the bone marrow (PubMed:24740813). Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction (PubMed:23169771). Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions (PubMed:15496415, PubMed:23169771). Required for normal organization of the lymph vessel network. Required for normal hematopoietic stem cell engraftment in the bone marrow. Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow. Promotes in vitro osteoblast proliferation and differentiation (By similarity). Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM. Mediates outgrowth and pathfinding for retinal ganglion cell axons (By similarity).
Isoform 3: Inhibits activities of membrane-bound isoforms by competing for the same interaction partners. Inhibits cell attachment via homotypic interactions. Promotes endothelial cell migration. Inhibits endothelial cell tube formation.
Biological Process
Adaptive immune response Source: UniProtKB-KW
Axon extension involved in axon guidance Source: UniProtKB
Cell adhesion Source: UniProtKB
Heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules Source: UniProtKB
Motor neuron axon guidance Source: Ensembl
Neuron projection extension Source: UniProtKB
Retinal ganglion cell axon guidance Source: UniProtKB
Signal transduction Source: ProtInc
Cellular Location
Cell membrane; Axon; Dendrite. Detected at the immunological synapse, i.e, at the contact zone between antigen-presenting dendritic cells and T-cells (PubMed:15294938, PubMed:16352806). Colocalizes with CD6 and the TCR/CD3 complex at the immunological synapse (PubMed:15294938).
Isoform 3: Secreted
Topology
Extracellular: 28-527 aa
Helical: 528-549 aa
Cytoplasmic: 550-583 aa
PTM
Glycosylated.

Zarghami, N., Soto, M. S., Perez-Balderas, F., Khrapitchev, A. A., Karali, C. S., Johanssen, V. A., ... & Sibson, N. R. (2021). A novel molecular magnetic resonance imaging agent targeting activated leukocyte cell adhesion molecule as demonstrated in mouse brain metastasis models. Journal of Cerebral Blood Flow & Metabolism, 41(7), 1592-1607.

Darvishi, B., Boroumandieh, S., Majidzadeh-A, K., Salehi, M., Jafari, F., & Farahmand, L. (2020). The role of activated leukocyte cell adhesion molecule (ALCAM) in cancer progression, invasion, metastasis and recurrence: A novel cancer stem cell marker and tumor-specific prognostic marker. Experimental and molecular pathology, 115, 104443.

Xie, S., Zhang, H., Wang, F., Liu, Y., Gao, K., Zhang, J., ... & Jiang, W. (2020). Activated leukocyte cell adhesion molecule as a biomarker for disease severity and efficacy of sublingual immunotherapy in allergic rhinitis. International Immunopharmacology, 88, 106975.

Achiha, T., Kijima, N., Kodama, Y., Kagawa, N., Kinoshita, M., Fujimoto, Y., ... & Kishima, H. (2020). Activated leukocyte cell adhesion molecule expression correlates with the WNT subgroup in medulloblastoma and is involved in regulating tumor cell proliferation and invasion. PloS one, 15(12), e0243272.

Sanders, A. J., Owen, S., Morgan, L. D., Ruge, F., Collins, R. J., Ye, L., ... & Jiang, W. G. (2019). Importance of activated leukocyte cell adhesion molecule (ALCAM) in prostate cancer progression and metastatic dissemination. Oncotarget, 10(59), 6362.

Ferragut, F., Cagnoni, A. J., Colombo, L. L., Terrero, C. S., Wolfenstein-Todel, C., Troncoso, M. F., ... & Elola, M. T. (2019). Dual knockdown of Galectin-8 and its glycosylated ligand, the activated leukocyte cell adhesion molecule (ALCAM/CD166), synergistically delays in vivo breast cancer growth. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1866(8), 1338-1352.

Oh, M. S., Hong, J. Y., Kim, M. N., Kwak, E. J., Kim, S. Y., Kim, E. G., ... & Sohn, M. H. (2019). Activated leukocyte cell adhesion molecule modulates Th2 immune response in atopic dermatitis. Allergy, asthma & immunology research, 11(5), 677-690.

Jeong, Y. J., Oh, H. K., Park, S. H., & Bong, J. G. (2018). Prognostic significance of activated leukocyte cell adhesion molecule (ALCAM) in association with promoter methylation of the ALCAM gene in breast cancer. Molecules, 23(1), 131.

Kim, M. N., Hong, J. Y., Shim, D. H., Sol, I. S., Kim, Y. S., Lee, J. H., ... & Sohn, M. H. (2018). Activated leukocyte cell adhesion molecule stimulates the T-cell response in allergic asthma. American journal of respiratory and critical care medicine, 197(8), 994-1008.

Devis, L., Moiola, C. P., Masia, N., Martinez‐Garcia, E., Santacana, M., Stirbat, T. V., ... & Colas, E. (2017). Activated leukocyte cell adhesion molecule (ALCAM) is a marker of recurrence and promotes cell migration, invasion, and metastasis in early‐stage endometrioid endometrial cancer. The Journal of pathology, 241(4), 475-487.

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For research use only. Not intended for any clinical use.

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