Mouse Anti-ATRIP Recombinant Antibody (EG314) (CBMAB-EN345-LY)

Name: Mouse Anti-ATRIP Recombinant Antibody (EG314)
SKU: CBMAB-EN345-LY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ATRIP Recombinant Antibody (EG314) in Immunoblot

Pulldown assays suggest that WT/mutant His-APE1 associated with ATR, ATRIP and RPA32 in PANC1 nuclear extracts. 'Input' or 'Pulldown' samples were examined via immunoblotting analysis as indicated. ...View More

Li, J., Zhao, H., McMahon, A., & Yan, S. (2022). APE1 assembles biomolecular condensates to promote the ATR-Chk1 DNA damage response in nucleolus. Nucleic Acids Research, 50(18), 10503-10525.

Mouse Anti-ATRIP Recombinant Antibody (EG314) in WB

Cells were incubated with vehicle or 1 μg/ml Dox for 72 h and then, where indicated, added 3 mM HU for 4 h (B), 6 h or 24 h (C). ...View More

Guerra, B., Doktor, T. K., Frederiksen, S. B., Somyajit, K., & Andresen, B. S. (2022). Essential role of CK2α for the interaction and stability of replication fork factors during DNA synthesis and activation of the S-phase checkpoint. Cellular and Molecular Life Sciences, 79(6), 339.

Mouse Anti-ATRIP Recombinant Antibody (EG314) in WB

PANC-1 cells were treated with 5 mmol/L shikonin, 200 nmol/L CPT or both for 0, 12, or 24 h, and protein expression was analyzed. The protein band intensity was analyzed using the Image J software and listed
above the corresponding gels. ...View More

Wang, F., Jin, S., Pozo, F. M., Tian, D., Tang, X., Dai, Y., ... & Zhang, Y. (2022). Chemical screen identifies shikonin as a broad DNA damage response inhibitor that enhances chemotherapy through inhibiting ATM and ATR. Acta Pharmaceutica Sinica B, 12(3), 1339-1350.

Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

EG314

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 492-791 mapping at the C-terminus of ATR-Interacting Protein of human origin.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

ATR Interacting Protein

Introduction

This gene encodes an essential component of the DNA damage checkpoint. The encoded protein binds to single-stranded DNA coated with replication protein A. The protein also interacts with the ataxia telangiectasia and Rad3 related protein kinase, resulting in its accumulation at intranuclear foci induced by DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2012]

Entrez Gene ID

84126

UniProt ID

Q8WXE1

Alternative Names

ATR Interacting Protein; ATM And Rad3-Related-Interacting Protein; AGS1;

Function

Required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein.

Biological Process

DNA damage checkpoint Source: GO_Central
DNA repair Source: GO_Central
DNA replication Source: Reactome
Interstrand cross-link repair Source: Reactome
Regulation of signal transduction by p53 class mediator Source: Reactome

Cellular Location

Nucleus. Redistributes to discrete nuclear foci upon DNA damage.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-ATRIP Recombinant Antibody (EG315) (CAT#: CBMAB-EN346-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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