Rabbit Anti-BBC3 Recombinant Antibody (CBAb027) (CBMAB-Ab027-LY)

The product is antibody recognizes BBC3. The antibody CBAb027 immunoassay techniques such as: WB 1:500-1:2000; IHC 1:50-1:200; IF 1:50-1:200.
See all BBC3 antibodies

Datasheet

Summary

Host Animal

Rabbit

Specificity

Human, Mouse, Rat

Clone

CBAb027

Antibody Isotype

IgG

Application

WB, IHC, IF

Basic Information

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-193 of human PUMA.

Host Species

Rabbit

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:500-1:1,000
IF(ICC)	1:50-1:200
IHC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

BCL2 Binding Component 3

Introduction

This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants.

Entrez Gene ID

Human	27113
Mouse	170770
Rat	317673

UniProt ID

Human	Q9BXH1
Mouse	Q99ML1
Rat	Q80ZG6

Alternative Names

BBC3; JFY-1; JFY1; PUMA; BCL2 binding component 3

Function

Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis (PubMed:11463391).
Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53. Regulates ER stress-induced neuronal apoptosis (PubMed:23340338).

Biological Process

Activation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
Apoptotic signaling pathway Source: UniProtKB
Cellular response to DNA damage stimulus Source: BHF-UCL
Cellular response to hypoxia Source: UniProtKB
Determination of adult lifespan Source: BHF-UCL
Execution phase of apoptosis Source: UniProtKB
Intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: ParkinsonsUK-UCL
Negative regulation of endoplasmic reticulum calcium ion concentration Source: UniProtKB
Negative regulation of growth Source: UniProtKB
Positive regulation of cysteine-type endopeptidase activity Source: UniProtKB
Positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway Source: ParkinsonsUK-UCL
Positive regulation of intrinsic apoptotic signaling pathway Source: UniProtKB
Positive regulation of IRE1-mediated unfolded protein response Source: ParkinsonsUK-UCL
Positive regulation of neuron apoptotic process Source: UniProtKB
Positive regulation of protein-containing complex assembly Source: UniProtKB
Positive regulation of protein homooligomerization Source: UniProtKB
Positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway Source: UniProtKB
Positive regulation of release of cytochrome c from mitochondria Source: BHF-UCL
Positive regulation of thymocyte apoptotic process Source: BHF-UCL
Protein insertion into mitochondrial membrane involved in apoptotic signaling pathway Source: Reactome
Regulation of apoptotic process Source: Reactome
Release of cytochrome c from mitochondria Source: UniProtKB
Release of sequestered calcium ion into cytosol Source: UniProtKB
Response to endoplasmic reticulum stress Source: UniProtKB

Cellular Location

Mitochondrion. Localized to the mitochondria in order to induce cytochrome c release.

References

Ma, B., Li, J., Yang, W. K., Zhang, M. G., Xie, X. D., & Bai, Z. T. (2021). N-trans-Feruloyloctopamine Wakes Up BBC3, DDIT3, CDKN1A, and NOXA Signals to Accelerate HCC Cell Apoptosis. Analytical Cellular Pathology, 2021.

Chen, W., & Li, X. (2020). MiR-222-3p promotes cell proliferation and inhibits apoptosis by targeting PUMA (BBC3) in non-small cell lung cancer. Technology in Cancer Research & Treatment, 19, 1533033820922558.

Hemati, H., Kaur, J., Sobti, R. C., & Trehanpati, N. (2020). Inhibition of NOTCH signaling pathway chemosensitizes HCC CD133+ cells to vincristine and 5-fluorouracil through upregulation of BBC3. Biochemical and biophysical research communications, 525(4), 941-947.

Fiori, M. E., Villanova, L., Barbini, C., De Angelis, M. L., & De Maria, R. (2018). miR-663 sustains NSCLC by inhibiting mitochondrial outer membrane permeabilization (MOMP) through PUMA/BBC3 and BTG2. Cell death & disease, 9(2), 1-13.

Liu, Z., Sun, J., Liu, B., Zhao, M., Xing, E., & Dang, C. (2018). miRNA‑222 promotes liver cancer cell proliferation, migration and invasion and inhibits apoptosis by targeting BBC3. International journal of molecular medicine, 42(1), 141-148.

Liu, H., Cheng, Y., Yang, J., Wang, W., Fang, S., Zhang, W., ... & Liao, H. (2017). BBC3 in macrophages promoted pulmonary fibrosis development through inducing autophagy during silicosis. Cell death & disease, 8(3), e2657-e2657.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

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Rabbit Anti-BBC3 Recombinant Antibody (CAP229)

Rabbit Anti-BBC3 Recombinant Antibody (E1S7A)

Mouse Anti-BBC3 Recombinant Antibody (10C5G1)

Mouse Anti-BBC3 Recombinant Antibody (CBYY-1838)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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