Mouse Anti-BBC3 Recombinant Antibody (CBYY-1838) (CBMAB-2187-YY)

This product is mouse antibody that recognizes BBC3. The antibody CBYY-1838 can be used for immunoassay techniques such as: ELISA, WB
See all BBC3 antibodies

Published Data

Mouse Anti-BBC3 Recombinant Antibody (CBYY-1838) (CBMAB-2187-YY) in IF

LRRK2 KO induces no activation of pro-apoptosis proteins under ROT stimuli. ...View More

Quintero-Espinosa, D. A., Sanchez-Hernandez, S., Velez-Pardo, C., Martin, F., & Jimenez-Del-Rio, M. (2023). LRRK2 Knockout Confers Resistance in HEK-293 Cells to Rotenone-Induced Oxidative Stress, Mitochondrial Damage, and Apoptosis. International Journal of Molecular Sciences, 24(13), 10474.

Mouse Anti-BBC3 Recombinant Antibody (CBYY-1838) (CBMAB-2187-YY) in WB

Western blotting was performed to evaluate the overexpression efficiency of pcDNA‑PUMA‑α. β‑actin was used as an internal control. ...View More

Gao, X., Yang, X., He, F., Liu, X., Liu, D., & Yuan, X. (2023). Downregulation of microRNA‑494 inhibits cell proliferation in lung squamous cell carcinoma via the induction of PUMA‑α‑mediated apoptosis. Experimental and Therapeutic Medicine, 25(6), 1-9.

Mouse Anti-BBC3 Recombinant Antibody (CBYY-1838) (CBMAB-2187-YY) in Immunoblot

HCT-116 cells were transfected with LC3B-specific siRNA or nonsilencing siRNA (NSRNA) as a control. After 48 h, BFT (300 ng/mL) was added to transfected and or untransfected cells for 6 h. The expression of LC3, p62, Bax, PUMA, and actin was analyzed by immunoblotting. ...View More

Ko, S. H., Choi, J. H., & Kim, J. M. (2023). Bacteroides fragilis Enterotoxin Induces Autophagy through an AMPK and FoxO3-Pathway, Leading to the Inhibition of Apoptosis in Intestinal Epithelial Cells. Toxins, 15(9), 544.

Mouse Anti-BBC3 Recombinant Antibody (CBYY-1838) (CBMAB-2187-YY) in WB

Aortic SMC were transfected with control (AD-shGFP) or C/EBPβsiRNA (AD-shC/EBPβ) followed by treatment with vehicle or BAPN+METH (1.0 mM) as indicated. PARP and caspase3 cleavage levels and IGFBP5/PUMA/p53 protein expression were detected by Western blot. ...View More

Luo, B. Y., Zhou, J., Guo, D., Yang, Q., Tian, Q., Cai, D. P., ... & Xie, W. B. (2022). Methamphetamine induces thoracic aortic aneurysm/dissection through C/EBPβ. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1868(9), 166447.

Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat, Cattle, Dog, Pig

Clone

CBYY-1838

Antibody Isotype

IgG1, κ

Application

WB, IP, IF, ELISA, IHC-P

Basic Information

Immunogen

Amino acids 2-29 at the N-terminus of PUMAα of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Cattle, Dog, Pig

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

BCL2 Binding Component 3

Introduction

This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants.

Entrez Gene ID

27113

UniProt ID

Q96PG8

Alternative Names

BCL2 Binding Component 3; P53 Up-Regulated Modulator Of Apoptosis; P53-Upregulated Modulator Of Apoptosis; JFY-1; PUMA; Bcl-2-Binding Component 3; JFY1;

Function

Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis (PubMed:11463391).
Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53. Regulates ER stress-induced neuronal apoptosis (PubMed:23340338).

Biological Process

Activation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
Apoptotic signaling pathway Source: UniProtKB
Cellular response to DNA damage stimulus Source: BHF-UCL
Cellular response to hypoxia Source: UniProtKB
Determination of adult lifespan Source: BHF-UCL
Execution phase of apoptosis Source: UniProtKB
Intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: ParkinsonsUK-UCL
Negative regulation of endoplasmic reticulum calcium ion concentration Source: UniProtKB
Negative regulation of growth Source: UniProtKB
Positive regulation of cysteine-type endopeptidase activity Source: UniProtKB
Positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway Source: ParkinsonsUK-UCL
Positive regulation of intrinsic apoptotic signaling pathway Source: UniProtKB
Positive regulation of IRE1-mediated unfolded protein response Source: ParkinsonsUK-UCL
Positive regulation of neuron apoptotic process Source: UniProtKB
Positive regulation of protein-containing complex assembly Source: UniProtKB
Positive regulation of protein homooligomerization Source: UniProtKB
Positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway Source: UniProtKB
Positive regulation of release of cytochrome c from mitochondria Source: BHF-UCL
Positive regulation of thymocyte apoptotic process Source: BHF-UCL
Protein insertion into mitochondrial membrane involved in apoptotic signaling pathway Source: Reactome
Regulation of apoptotic process Source: Reactome
Release of cytochrome c from mitochondria Source: UniProtKB
Release of sequestered calcium ion into cytosol Source: UniProtKB
Response to endoplasmic reticulum stress Source: UniProtKB

Cellular Location

Mitochondrion. Localized to the mitochondria in order to induce cytochrome c release.

References

Ma, B., Li, J., Yang, W. K., Zhang, M. G., Xie, X. D., & Bai, Z. T. (2021). N-trans-Feruloyloctopamine Wakes Up BBC3, DDIT3, CDKN1A, and NOXA Signals to Accelerate HCC Cell Apoptosis. Analytical Cellular Pathology, 2021.

Chen, W., & Li, X. (2020). MiR-222-3p promotes cell proliferation and inhibits apoptosis by targeting PUMA (BBC3) in non-small cell lung cancer. Technology in Cancer Research & Treatment, 19, 1533033820922558.

Hemati, H., Kaur, J., Sobti, R. C., & Trehanpati, N. (2020). Inhibition of NOTCH signaling pathway chemosensitizes HCC CD133+ cells to vincristine and 5-fluorouracil through upregulation of BBC3. Biochemical and biophysical research communications, 525(4), 941-947.

Fiori, M. E., Villanova, L., Barbini, C., De Angelis, M. L., & De Maria, R. (2018). miR-663 sustains NSCLC by inhibiting mitochondrial outer membrane permeabilization (MOMP) through PUMA/BBC3 and BTG2. Cell death & disease, 9(2), 1-13.

Liu, Z., Sun, J., Liu, B., Zhao, M., Xing, E., & Dang, C. (2018). miRNA‑222 promotes liver cancer cell proliferation, migration and invasion and inhibits apoptosis by targeting BBC3. International journal of molecular medicine, 42(1), 141-148.

Liu, H., Cheng, Y., Yang, J., Wang, W., Fang, S., Zhang, W., ... & Liao, H. (2017). BBC3 in macrophages promoted pulmonary fibrosis development through inducing autophagy during silicosis. Cell death & disease, 8(3), e2657-e2657.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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