Rabbit Anti-CDC25B Recombinant Antibody (
3D6) (V2LY-0725-LY649)

Name: Rabbit Anti-CDC25B Recombinant Antibody (3D6)
SKU: V2LY-0725-LY649
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

3D6

Application

ELISA

Immunogen

A synthesized peptide derived from human CDC25B.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

cell division cycle 25 homolog B (S. pombe)

Entrez Gene ID

994

UniProt ID

P30305

Function

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.

Biological Process

Cell division Source: UniProtKB-KW
Female meiosis I Source: Ensembl
G2/M transition of mitotic cell cycle Source: GO_Central
Mitotic cell cycle Source: UniProtKB
Oocyte maturation Source: Ensembl
Positive regulation of cell population proliferation Source: ProtInc
Positive regulation of cytokinesis Source: UniProtKB
Positive regulation of G2/MI transition of meiotic cell cycle Source: GO_Central
Positive regulation of G2/M transition of mitotic cell cycle Source: GO_Central
Positive regulation of mitotic cell cycle Source: UniProtKB
Positive regulation of protein kinase activity Source: Ensembl
Protein phosphorylation Source: UniProtKB

Cellular Location

Centrosome; Spindle pole

PTM

Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins.

Li, H. X., Yang, W. Y., Li, L. P., Zhou, H., Li, W. Y., Ma, Y., & Wang, R. L. (2021). Molecular dynamics study of CDC25BR492L mutant causing the activity decrease of CDC25B. Journal of Molecular Graphics and Modelling, 109, 108030.

Liu, J., Yu, X., Yu, H., Liu, B., Zhang, Z., Kong, C., & Li, Z. (2020). Knockdown of MAPK14 inhibits the proliferation and migration of clear cell renal cell carcinoma by downregulating the expression of CDC25B. Cancer medicine, 9(3), 1183-1195.

Cerchia, C., Nasso, R., Mori, M., Villa, S., Gelain, A., Capasso, A., ... & Lavecchia, A. (2019). Discovery of novel naphthylphenylketone and naphthylphenylamine derivatives as cell division cycle 25B (CDC25B) phosphatase inhibitors: design, synthesis, inhibition mechanism, and in vitro efficacy against melanoma cell lines. Journal of medicinal chemistry, 62(15), 7089-7110.

Al‐Matouq, J., Holmes, T. R., & Hansen, L. A. (2019). CDC25B and CDC25C overexpression in nonmelanoma skin cancer suppresses cell death. Molecular carcinogenesis, 58(9), 1691-1700.

Xiao, Y., Yu, Y., Gao, D., Jin, W., Jiang, P., Li, Y., ... & Zhang, R. (2019). Inhibition of CDC25B With WG-391D impedes the tumorigenesis of ovarian cancer. Frontiers in oncology, 9, 236.

Cui, L., Mahesutihan, M., Zheng, W., Meng, L., Fan, W., Li, J., ... & Sun, L. (2018). CDC25B promotes influenza A virus replication by regulating the phosphorylation of nucleoprotein. Virology, 525, 40-47.

Wang, D., Si, S., Wang, Q., Luo, G., Du, Q., Liang, Q., ... & Leng, Z. (2018). MiR-27a promotes hemin-induced erythroid differentiation of K562 cells by targeting CDC25B. Cellular Physiology and Biochemistry, 46(1), 365-374.

Castro‐Sánchez, P., Ramirez‐Munoz, R., Lamana, A., Ortiz, A., González‐Álvaro, I., & Roda‐Navarro, P. (2017). mRNA profilin identifies low levels of phosphatases dual‐specific phosphatase‐7 (DUSP7) and cell division cycle‐25B (CDC25B) in patients with early arthritis. Clinical & Experimental Immunology, 189(1), 113-119.

Zhong, Y., Yang, J., Xu, W. W., Wang, Y., Zheng, C. C., Li, B., & He, Q. Y. (2017). KCTD12 promotes tumorigenesis by facilitating CDC25B/CDK1/Aurora A-dependent G2/M transition. Oncogene, 36(44), 6177-6189.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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