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Rabbit Anti-CDC25B Recombinant Antibody (EG561) (CBMAB-EN678-LY)


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Summary

Host Animal
Rabbit
Specificity
Human
Clone
EG561
Antibody Isotype
IgG
Application
WB

Basic Information

Immunogen
Recombinant protein within Human Cdc25B aa 171-220.
Host Species
Rabbit
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
TBS, pH 7.4, 40% Glycerol, 0.05% BSA
Preservative
0.05% sodium azide
Concentration
1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Cell Division Cycle 25B
Introduction
CDC25B (Cell Division Cycle 25B) is a Protein Coding gene. Diseases associated with CDC25B include Breast Cancer. Among its related pathways are p38 signaling mediated by MAPKAP kinases and MAPK signaling pathway. Gene Ontology (GO) annotations related to this gene include protein kinase binding and protein tyrosine phosphatase activity. An important paralog of this gene is CDC25A.
Entrez Gene ID
Human994
Mouse12531
Rat171103
UniProt ID
HumanP30305
MouseP30306
RatP48966
Alternative Names
Cell Division Cycle 25B; EC 3.1.3.48; Dual Specificity Phosphatase Cdc25B; CDC25HU2;
Function
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
Biological Process
Cell division Source: UniProtKB-KW
Female meiosis I Source: Ensembl
G2/M transition of mitotic cell cycle Source: GO_Central
Mitotic cell cycle Source: UniProtKB
Oocyte maturation Source: Ensembl
Positive regulation of cell population proliferation Source: ProtInc
Positive regulation of cytokinesis Source: UniProtKB
Positive regulation of G2/MI transition of meiotic cell cycle Source: GO_Central
Positive regulation of G2/M transition of mitotic cell cycle Source: GO_Central
Positive regulation of mitotic cell cycle Source: UniProtKB
Positive regulation of protein kinase activity Source: Ensembl
Protein phosphorylation Source: UniProtKB
Cellular Location
Centrosome; Spindle pole
PTM
Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins.
More Infomation

Li, H. X., Yang, W. Y., Li, L. P., Zhou, H., Li, W. Y., Ma, Y., & Wang, R. L. (2021). Molecular dynamics study of CDC25BR492L mutant causing the activity decrease of CDC25B. Journal of Molecular Graphics and Modelling, 109, 108030.

Liu, J., Yu, X., Yu, H., Liu, B., Zhang, Z., Kong, C., & Li, Z. (2020). Knockdown of MAPK14 inhibits the proliferation and migration of clear cell renal cell carcinoma by downregulating the expression of CDC25B. Cancer medicine, 9(3), 1183-1195.

Cerchia, C., Nasso, R., Mori, M., Villa, S., Gelain, A., Capasso, A., ... & Lavecchia, A. (2019). Discovery of novel naphthylphenylketone and naphthylphenylamine derivatives as cell division cycle 25B (CDC25B) phosphatase inhibitors: design, synthesis, inhibition mechanism, and in vitro efficacy against melanoma cell lines. Journal of medicinal chemistry, 62(15), 7089-7110.

Al‐Matouq, J., Holmes, T. R., & Hansen, L. A. (2019). CDC25B and CDC25C overexpression in nonmelanoma skin cancer suppresses cell death. Molecular carcinogenesis, 58(9), 1691-1700.

Xiao, Y., Yu, Y., Gao, D., Jin, W., Jiang, P., Li, Y., ... & Zhang, R. (2019). Inhibition of CDC25B With WG-391D impedes the tumorigenesis of ovarian cancer. Frontiers in oncology, 9, 236.

Cui, L., Mahesutihan, M., Zheng, W., Meng, L., Fan, W., Li, J., ... & Sun, L. (2018). CDC25B promotes influenza A virus replication by regulating the phosphorylation of nucleoprotein. Virology, 525, 40-47.

Wang, D., Si, S., Wang, Q., Luo, G., Du, Q., Liang, Q., ... & Leng, Z. (2018). MiR-27a promotes hemin-induced erythroid differentiation of K562 cells by targeting CDC25B. Cellular Physiology and Biochemistry, 46(1), 365-374.

Castro‐Sánchez, P., Ramirez‐Munoz, R., Lamana, A., Ortiz, A., González‐Álvaro, I., & Roda‐Navarro, P. (2017). mRNA profilin identifies low levels of phosphatases dual‐specific phosphatase‐7 (DUSP7) and cell division cycle‐25B (CDC25B) in patients with early arthritis. Clinical & Experimental Immunology, 189(1), 113-119.

Zhong, Y., Yang, J., Xu, W. W., Wang, Y., Zheng, C. C., Li, B., & He, Q. Y. (2017). KCTD12 promotes tumorigenesis by facilitating CDC25B/CDK1/Aurora A-dependent G2/M transition. Oncogene, 36(44), 6177-6189.

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For research use only. Not intended for any clinical use.

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