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Mouse Anti-CLDN5 Recombinant Antibody (CBYY-C3191) (V2LY-1206-LY50)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYY-C3191
Antibody Isotype
IgG1
Application
IHC-P, WB

Basic Information

Immunogen
A synthetic peptide of human Claudin 5.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB0.1-1 µg/ml
IHC-P2-10 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
0.01% sodium azide
Concentration
0.5 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
claudin 5
Entrez Gene ID
7122
UniProt ID
O00501
Function
Plays a major role in tight junction-specific obliteration of the intercellular space.
Biological Process
Bicellular tight junction assembly Source: GO_Central
Calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules Source: UniProtKB
Cell adhesion Source: GO_Central
Cell-cell junction assembly Source: UniProtKB
Establishment of blood-retinal barrier Source: ARUK-UCL
Face morphogenesis Source: UniProtKB
Learning Source: UniProtKB
Maintenance of blood-brain barrier Source: ARUK-UCL
Negative regulation of angiogenesis Source: ARUK-UCL
Negative regulation of cell migration Source: ARUK-UCL
Negative regulation of gene expression Source: ARUK-UCL
Negative regulation of vascular permeability Source: ARUK-UCL
Outflow tract morphogenesis Source: UniProtKB
Positive regulation of bicellular tight junction assembly Source: ARUK-UCL
Positive regulation of cell population proliferation Source: ARUK-UCL
Positive regulation of establishment of endothelial barrier Source: UniProtKB
Positive regulation of gene expression Source: ARUK-UCL
Positive regulation of protein binding Source: ARUK-UCL
Regulation of bicellular tight junction assembly Source: Reactome
Roof of mouth development Source: UniProtKB
Tight junction assembly Source: ARUK-UCL
Transforming growth factor beta receptor signaling pathway Source: UniProtKB
Cellular Location
Cell membrane; Tight junction
Topology
Cytoplasmic: 1-7
Helical: 8-28
Extracellular: 29-81
Helical: 82-102
Cytoplasmic: 103-122
Helical: 123-143
Extracellular: 144-159
Helical: 160-180
Cytoplasmic: 181-218
More Infomation

Yang, Z., Lin, P., Chen, B., Zhang, X., Xiao, W., Wu, S., ... & Zhang, J. (2021). Autophagy alleviates hypoxia-induced blood-brain barrier injury via regulation of CLDN5 (claudin 5). Autophagy, 17(10), 3048-3067.

Chiba, H., Ichikawa‐Tomikawa, N., Imura, T., & Sugimoto, K. (2021). The region‐selective regulation of endothelial claudin‐5 expression and signaling in brain health and disorders. Journal of Cellular Physiology.

Tachibana, K., Hashimoto, Y., Shirakura, K., Okada, Y., Hirayama, R., Iwashita, Y., ... & Kondoh, M. (2021). Safety and efficacy of an anti-claudin-5 monoclonal antibody to increase blood–brain barrier permeability for drug delivery to the brain in a non-human primate. Journal of Controlled Release.

Berndt, P., Winkler, L., Cording, J., Breitkreuz-Korff, O., Rex, A., Dithmer, S., ... & Haseloff, R. F. (2019). Tight junction proteins at the blood–brain barrier: far more than claudin-5. Cellular and molecular life sciences, 76(10), 1987-2002.

Hashimoto, Y., Zhou, W., Hamauchi, K., Shirakura, K., Doi, T., Yagi, K., ... & Takeda, H. (2018). Engineered membrane protein antigens successfully induce antibodies against extracellular regions of claudin-5. Scientific reports, 8(1), 1-12.

Zhang, X., Chen, J., Sun, L., & Xu, Y. (2018). SIRT1 deacetylates KLF4 to activate Claudin‐5 transcription in ovarian cancer cells. Journal of cellular biochemistry, 119(2), 2418-2426.

Neuhaus, W., Piontek, A., Protze, J., Eichner, M., Mahringer, A., Subileau, E. A., ... & Piontek, J. (2018). Reversible opening of the blood-brain barrier by claudin-5-binding variants of Clostridium perfringens enterotoxin's claudin-binding domain. Biomaterials, 161, 129-143.

Hashimoto, Y., Shirakura, K., Okada, Y., Takeda, H., Endo, K., Tamura, M., ... & Kondoh, M. (2017). Claudin-5-binders enhance permeation of solutes across the blood-brain barrier in a mammalian model. Journal of Pharmacology and Experimental Therapeutics, 363(2), 275-283.

Ma, S. C., Li, Q., Peng, J. Y., Zhouwen, J. L., Diao, J. F., Niu, J. X., ... & Jiang, W. G. (2017). Claudin‐5 regulates blood‐brain barrier permeability by modifying brain microvascular endothelial cell proliferation, migration, and adhesion to prevent lung cancer metastasis. CNS neuroscience & therapeutics, 23(12), 947-960.

Nishiura, K., Ichikawa-Tomikawa, N., Sugimoto, K., Kunii, Y., Kashiwagi, K., Tanaka, M., ... & Chiba, H. (2017). PKA activation and endothelial claudin-5 breakdown in the schizophrenic prefrontal cortex. Oncotarget, 8(55), 93382.

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For research use only. Not intended for any clinical use.

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