Mouse Anti-CPLX3 Recombinant Antibody (CBFYC-2158) (V2LY-1206-LY959)

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Basic Information

Host Animal
Mouse
Clone
CBFYC-2158
Application
WB, IP, IF, ELISA
Immunogen
Amino acids 1-44 mapping at the N-terminus of human Complexin-3.
Host Species
Mouse
Specificity
Human, Mouse, Rat, Dog, Cattle, Pig, Horse
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
0.1% gelatin
Preservative
0.09% sodium azide
Concentration
0.2 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
Complexin 3
Entrez Gene ID
Mouse235415
Rat501005
UniProt ID
MouseQ8R1B5
RatD4ABY0
Function
Complexin that regulates SNARE protein complex-mediated synaptic vesicle fusion (By similarity).

Required for the maintenance of synaptic ultrastructure in the adult retina (By similarity).

Positively regulates synaptic transmission through synaptic vesicle availability and exocytosis of neurotransmitters at photoreceptor ribbon synapses in the retina (By similarity).

Suppresses tonic photoreceptor activity and baseline 'noise' by suppression of Ca2+ vesicle tonic release and the facilitation of evoked synchronous and asynchronous Ca2+ vesicle release (By similarity).
Biological Process
Insulin secretion Source: Ensembl
Regulation of neurotransmitter secretion Source: GO_Central
Regulation of synaptic vesicle fusion to presynaptic active zone membrane Source: Ensembl
Response to stimulus Source: UniProtKB-KW
Synaptic vesicle exocytosis Source: GO_Central
Visual perception Source: UniProtKB-KW
Cellular Location
Cell membrane; Synapse. Enriched at the synaptic terminal (By similarity). Localized at glycinergic synaptic contacts of AII amacrine cells with OFF cone bipolar cells in the OFF sublamina of the retina inner nuclear layer (By similarity).
PTM
Farnesylation mediates presynaptic targeting.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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