Human Recombinant CPLX3 protein, His Tag (V2LY-0526-LY3350)

Name: Human Recombinant CPLX3 protein, His Tag
SKU: V2LY-0526-LY3350
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant CPLX3 protein, His Tag consist of Amino Acid: 1-154 and predicts a molecular mass of 19.5 kDa.

Molecule Mass

19.5 kDa

Sequence

Amino Acid: 1-154

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Complexin 3

Function

Complexin that regulates SNARE protein complex-mediated synaptic vesicle fusion (By similarity).

Required for the maintenance of synaptic ultrastructure in the adult retina (By similarity).

Positively regulates synaptic transmission through synaptic vesicle availability and exocytosis of neurotransmitters at photoreceptor ribbon synapses in the retina (By similarity).

Suppresses tonic photoreceptor activity and baseline 'noise' by suppression of Ca2+ vesicle tonic release and the facilitation of evoked synchronous and asynchronous Ca2+ vesicle release (By similarity).

Biological Process

Insulin secretion Source: Ensembl
Regulation of neurotransmitter secretion Source: GO_Central
Regulation of synaptic vesicle fusion to presynaptic active zone membrane Source: Ensembl
Response to stimulus Source: UniProtKB-KW
Synaptic vesicle exocytosis Source: GO_Central
Visual perception Source: UniProtKB-KW

Cellular Location

Cell membrane; Synapse. Enriched at the synaptic terminal (By similarity). Localized at glycinergic synaptic contacts of AII amacrine cells with OFF cone bipolar cells in the OFF sublamina of the retina inner nuclear layer (By similarity).

PTM

Farnesylation mediates presynaptic targeting.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-CPLX3 Recombinant Antibody (CBFYC-2158) (CAT#: V2LY-1206-LY959)

Isotype control

For research use only. Not intended for any clinical use.

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