Mouse Anti-EDEM2 Recombinant Antibody (2E4) (CBMAB-C5000-LY)

Name: Mouse Anti-EDEM2 Recombinant Antibody (2E4)
SKU: CBMAB-C5000-LY
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

2E4

Antibody Isotype

IgG1, κ

Application

WB, ELISA, IHC-P, IP

Basic Information

Immunogen

C20orf31 (aa479-578) partial recombinant protein.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IHC-P	3 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

ER Degradation Enhancing Alpha-Mannosidase Like Protein 2

Entrez Gene ID

55741

UniProt ID

Q9BV94

Research Area

Involved in the endoplasmic reticulum-associated degradation (ERAD) pathway that targets misfolded glycoproteins for degradation in an N-glycan-dependent manner (PubMed:15537790, PubMed:25092655).

May initiate ERAD by promoting the first mannose trimming step of ERAD substrates, from Man9GlcNAc2 to Man8GlcNAc2 (PubMed:25092655).

Seems to recognize and bind to exposed hydrophobic regions in target proteins (By similarity).

Biological Process

Carbohydrate metabolic process Source: InterPro
Mannose trimming involved in glycoprotein ERAD pathway Source: ParkinsonsUK-UCL
Positive regulation of retrograde protein transport, ER to cytosol Source: ParkinsonsUK-UCL
Response to unfolded protein Source: UniProtKB-KW
Trimming of first mannose on A branch Source: Reactome
Trimming of second mannose on A branch Source: Reactome
Trimming of terminal mannose on B branch Source: ParkinsonsUK-UCL
Trimming of terminal mannose on C branch Source: Reactome
Ubiquitin-dependent glycoprotein ERAD pathway Source: ParkinsonsUK-UCL
Viral protein processing Source: Reactome

Cellular Location

Endoplasmic reticulum lumen

PTM

N-glycosylated.

More Infomation

References

Raja, R., & Liu, W. (2022). EDEM2 regulation of cardiac lipid metabolism in heart failure with preserved ejection fraction. Cardiovascular Research, 118(Supplement_1), cvac066-099.

Munteanu, C. V., Chirițoiu, G. N., Chirițoiu, M., Ghenea, S., Petrescu, A. J., & Petrescu, Ş. M. (2021). Affinity proteomics and deglycoproteomics uncover novel EDEM2 endogenous substrates and an integrative ERAD network. Molecular & Cellular Proteomics, 20.

Chirițoiu, G. N., Chirițoiu, M., & Munteanu, C. V. (2021). Dataset of human EDEM2 melanoma cells proteomics, affinity proteomics and deglycoproteomics. Data in Brief, 39, 107471.

George, G., Ninagawa, S., Yagi, H., Saito, T., Ishikawa, T., Sakuma, T., ... & Mori, K. (2020). EDEM2 stably disulfide-bonded to TXNDC11 catalyzes the first mannose trimming step in mammalian glycoprotein ERAD. Elife, 9, e53455.

Alhaidan, Y., Christesen, H. T., Højlund, K., Al Balwi, M. A., & Brusgaard, K. (2020). A novel gene in early childhood diabetes: EDEM2 silencing decreases SLC2A2 and PXD1 expression, leading to impaired insulin secretion. Molecular Genetics and Genomics, 295(5), 1253-1262.

Shenkman, M., Ron, E., Yehuda, R., Benyair, R., Khalaila, I., & Lederkremer, G. Z. (2018). Mannosidase activity of EDEM1 and EDEM2 depends on an unfolded state of their glycoprotein substrates. Communications Biology, 1(1), 1-11.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-EDEM2 Recombinant Antibody (CBFYE-0436)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
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