Sign in or Register   Sign in or Register
  |  

Mouse Anti-GRK2 Recombinant Antibody (CBYC-A972) (CBMAB-A4452-YC)

Provided herein is a Mouse monoclonal antibody against Human G Protein-Coupled Receptor Kinase 2. The antibody can be used for immunoassay techniques, such as ELISA, IF, IHC, IP, WB.
See all GRK2 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYC-A972
Antibody Isotype
IgG2b
Application
ELISA, IF, IHC, IP, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid in PBS, pH 7.2
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
G protein-coupled receptor kinase 2
Introduction
GRK2 is a member of the G protein-coupled receptor kinase family of proteins. The encoded protein phosphorylates the beta-adrenergic receptor as well as a wide range of other substrates including non-GPCR cell surface receptors, and cytoskeletal, mitochon
Entrez Gene ID
156
UniProt ID
P25098
Alternative Names
G Protein-Coupled Receptor Kinase 2; EC 2.7.11.15; Beta-ARK-1; ADRBK1; BARK1; Adrenergic, Beta, Receptor Kinase 1; G-Protein Coupled Receptor Kinase 2;
Function
Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them (PubMed:19715378).

Key regulator of LPAR1 signaling (PubMed:19306925).

Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor (PubMed:19306925).

Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner (PubMed:19306925).

Positively regulates ciliary smoothened (SMO)-dependent Hedgehog (Hh) signaling pathway by facilitating the trafficking of SMO into the cilium and the stimulation of SMO activity (By similarity).

Inhibits relaxation of airway smooth muscle in response to blue light (PubMed:30284927).
Biological Process
Cardiac muscle contraction Source: BHF-UCL
Desensitization of G protein-coupled receptor signaling pathway Source: BHF-UCL
G protein-coupled acetylcholine receptor signaling pathway Source: BHF-UCL
G protein-coupled receptor signaling pathway Source: GO_Central
Heart development Source: Ensembl
Negative regulation of relaxation of smooth muscle Source: UniProtKB
Negative regulation of striated muscle contraction Source: BHF-UCL
Negative regulation of the force of heart contraction by chemical signal Source: BHF-UCL
Peptidyl-serine phosphorylation Source: BHF-UCL
Peptidyl-threonine phosphorylation Source: Ensembl
Positive regulation of catecholamine secretion Source: BHF-UCL
Protein phosphorylation Source: GO_Central
Receptor internalization Source: UniProtKB
Regulation of signal transduction Source: GO_Central
Regulation of the force of heart contraction Source: GO_Central
Tachykinin receptor signaling pathway Source: BHF-UCL
Viral entry into host cell Source: CACAO
Viral genome replication Source: CACAO
Cellular Location
Cell membrane; Cytoplasm

Davis, K., Mitchell, C., Weissenfels, O., Bai, J., Raizen, D. M., Ailion, M., & Topalidou, I. (2023). G protein-coupled receptor kinase-2 (GRK-2) controls exploration through neuropeptide signaling in Caenorhabditis elegans. PLoS genetics, 19(1), e1010613.

Crudden, C., Shibano, T., Song, D., Dragomir, M. P., Cismas, S., Serly, J., ... & Girnita, L. (2021). Inhibition of G Protein–Coupled Receptor Kinase 2 Promotes Unbiased Downregulation of IGF1 Receptor and Restrains Malignant Cell GrowthUnbiased IGF1R Downregulation through GRK2 Inhibition. Cancer research, 81(2), 501-514.

Xu, G., Gaul, M. D., Liu, Z., DesJarlais, R. L., Qi, J., Wang, W., ... & Spurlino, J. C. (2020). Hit-to-lead optimization and discovery of a potent, and orally bioavailable G protein coupled receptor kinase 2 (GRK2) inhibitor. Bioorganic & Medicinal Chemistry Letters, 30(23), 127602.

Kang, J. H., Toita, R., Kawano, T., Murata, M., & Asai, D. (2020). Design of substrates and inhibitors of G protein-coupled receptor kinase 2 (GRK2) based on its phosphorylation reaction. Amino Acids, 52, 863-870.

Gambardella, J., Sorriento, D., Bova, M., Rusciano, M., Loffredo, S., Wang, X., ... & Ciccarelli, M. (2020). Role of endothelial G protein-coupled receptor kinase 2 in angioedema. Hypertension, 76(5), 1625-1636.

Murga, C., Arcones, A. C., Cruces-Sande, M., Briones, A. M., Salaices, M., & Mayor Jr, F. (2019). G protein-coupled receptor kinase 2 (GRK2) as a potential therapeutic target in cardiovascular and metabolic diseases. Frontiers in pharmacology, 10, 112.

Penela, P., Ribas, C., Sánchez-Madrid, F., & Mayor, F. (2019). G protein-coupled receptor kinase 2 (GRK2) as a multifunctional signaling hub. Cellular and Molecular Life Sciences, 76, 4423-4446.

Mayor Jr, F., Cruces-Sande, M., Arcones, A. C., Vila-Bedmar, R., Briones, A. M., Salaices, M., & Murga, C. (2018). G protein-coupled receptor kinase 2 (GRK2) as an integrative signalling node in the regulation of cardiovascular function and metabolic homeostasis. Cellular Signalling, 41, 25-32.

Cruces-Sande, M., Vila-Bedmar, R., Arcones, A. C., Gonzalez-Rodriguez, A., Rada, P., Gutierrez-de-Juan, V., ... & Murga, C. (2018). Involvement of G protein-coupled receptor kinase 2 (GRK2) in the development of non-alcoholic steatosis and steatohepatitis in mice and humans. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1864(12), 3655-3667.

Pfleger, J., Gross, P., Johnson, J., Carter, R. L., Gao, E., Tilley, D. G., ... & Koch, W. J. (2018). G protein-coupled receptor kinase 2 contributes to impaired fatty acid metabolism in the failing heart. Journal of molecular and cellular cardiology, 123, 108-117.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-GRK2 Recombinant Antibody (CBYC-A972)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare