Mouse Anti-HELLS Recombinant Antibody (1D10) (CBMAB-A3819-LY)

Name: Mouse Anti-HELLS Recombinant Antibody (1D10)
SKU: CBMAB-A3819-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1D10

Application

WB, ELISA

Immunogen

HELLS (AAH30963, 1 a.a. ~ 348 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

HELLS

Introduction

This gene encodes a lymphoid-specific helicase. Other helicases function in processes involving DNA strand separation, including replication, repair, recombination, and transcription. This protein is thought to be involved with cellular proliferation and may play a role in leukemogenesis. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]

Entrez Gene ID

3070

UniProt ID

Q9NRZ9

Alternative Names

FLJ10339; LSH; Nbla10143; PASG; SMARCA6

Function

Plays an essential role in normal development and survival. Involved in regulation of the expansion or survival of lymphoid cells. Required for de novo or maintenance DNA methylation. May control silencing of the imprinted CDKN1C gene through DNA methylation. May play a role in formation and organization of heterochromatin, implying a functional role in the regulation of transcription and mitosis (By similarity).

Biological Process

Cell cycle Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Cellular response to leukemia inhibitory factor Source: Ensembl
DNA methylation Source: GO_Central
DNA methylation-dependent heterochromatin assembly Source: UniProtKB
Kidney development Source: UniProtKB
Lymphocyte proliferation Source: UniProtKB
Maintenance of DNA methylation Source: UniProtKB
Negative regulation of intrinsic apoptotic signaling pathway Source: Ensembl
Pericentric heterochromatin assembly Source: UniProtKB

Cellular Location

Nucleus. Closely associated with pericentric heterochromatin.

Involvement in disease

Immunodeficiency-centromeric instability-facial anomalies syndrome 4 (ICF4):
A rare disorder characterized by a variable immunodeficiency resulting in recurrent infections, facial anomalies, and branching of chromosomes 1, 9, and 16. Other variable symptoms include growth retardation, failure to thrive, and psychomotor retardation. Laboratory studies show limited hypomethylation of DNA in a small fraction of the genome in some, but not all, patients.

Spruce, C., Dlamini, S., Ananda, G., Bronkema, N., Tian, H., Paigen, K., ... & Baker, C. L. (2020). HELLS and PRDM9 form a pioneer complex to open chromatin at meiotic recombination hot spots. Genes & development, 34(5-6), 398-412.

Law, C. T., Wei, L., Tsang, F. H. C., Chan, C. Y. K., Xu, I. M. J., Lai, R. K. H., ... & Wong, C. M. (2019). HELLS regulates chromatin remodeling and epigenetic silencing of multiple tumor suppressor genes in human hepatocellular carcinoma. Hepatology, 69(5), 2013-2030.

Velasco, G., Grillo, G., Touleimat, N., Ferry, L., Ivkovic, I., Ribierre, F., ... & Francastel, C. (2018). Comparative methylome analysis of ICF patients identifies heterochromatin loci that require ZBTB24, CDCA7 and HELLS for their methylated state. Human molecular genetics, 27(14), 2409-2424.

Jenness, C., Giunta, S., Müller, M. M., Kimura, H., Muir, T. W., & Funabiki, H. (2018). HELLS and CDCA7 comprise a bipartite nucleosome remodeling complex defective in ICF syndrome. Proceedings of the National Academy of Sciences, 115(5), E876-E885.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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