Mouse Anti-MCC (AA 5-146) Recombinant Antibody (1/MCC) (CBMAB-M2033-FY)

Name: Mouse Anti-MCC (AA 5-146) Recombinant Antibody (1/MCC)
SKU: CBMAB-M2033-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1/MCC

Application

WB, IF

Immunogen

Human MCC aa. 5-146

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Concentration

0.25 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 5-146

More Infomation

Target

Full Name

Mutated In Colorectal Cancers

Introduction

This gene is a candidate colorectal tumor suppressor gene that is thought to negatively regulate cell cycle progression. The orthologous gene in the mouse expresses a phosphoprotein associated with the plasma membrane and membrane organelles, and overexpression of the mouse protein inhibits entry into S phase. Multiple transcript variants encoding different isoforms have been found for this gene.

Entrez Gene ID

Human	4163
Mouse	328949
Rat	307449

UniProt ID

Human	P23508
Mouse	E9PWI3
Rat	F1LZS6

Alternative Names

Mutated In Colorectal Cancers; Colorectal Mutant Cancer Protein; Protein MCC; MCC1

Function

Candidate for the putative colorectal tumor suppressor gene located at 5q21. Suppresses cell proliferation and the Wnt/b-catenin pathway in colorectal cancer cells. Inhibits DNA binding of b-catenin/TCF/LEF transcription factors. Involved in cell migration independently of RAC1, CDC42 and p21-activated kinase (PAK) activation (PubMed:18591935, PubMed:19555689, PubMed:22480440).

Represses the beta-catenin pathway (canonical Wnt signaling pathway) in a CCAR2-dependent manner by sequestering CCAR2 to the cytoplasm, thereby impairing its ability to inhibit SIRT1 which is involved in the deacetylation and negative regulation of beta-catenin (CTNB1) transcriptional activity (PubMed:24824780).

Biological Process

Establishment of protein localization Source: CACAO
Negative regulation of canonical Wnt signaling pathway Source: UniProtKB
Negative regulation of epithelial cell migration Source: UniProtKB
Negative regulation of epithelial cell proliferation Source: UniProtKB
Signal transduction Source: ProtInc
Wnt signaling pathway Source: UniProtKB-KW

Cellular Location

Cytoplasm
Plasma membrane
Cell membrane
Nucleus
Other locations
lamellipodium
Note: Colocalizes with actin at the leading edge of polarized cells.

Zhou, H. M., & Zhao, L. M. (2023). Wnt signaling pathway-derived score for predicting therapeutic resistance and tumor microenvironment in lung adenocarcinoma. Frontiers in Pharmacology, 13, 1091018.

Tomaz, L. B., Liu, B. A., Meroshini, M., Ong, S. L., Tan, E. K., Tolwinski, N. S., ... & Dunn, N. R. (2022). MCC is a centrosomal protein that relocalizes to non-centrosomal apical sites during intestinal cell differentiation. Journal of Cell Science, 135(21), jcs259272.

Song, H., Zhuang, L., Xu, X., Shi, J., Hu, W., Liu, Z., & Shi, S. (2021). MCC Regulator of WNT Signaling Pathway (MCC) Is a Podocyte Essential Gene. Frontiers in Medicine, 8, 777563.

Liu, Z., Wang, P., Wold, E. A., Song, Q., Zhao, C., Wang, C., & Zhou, J. (2021). Small-molecule inhibitors targeting the canonical WNT signaling pathway for the treatment of cancer. Journal of medicinal chemistry, 64(8), 4257-4288.

Oss‐Ronen, L., & Cohen, I. (2021). Epigenetic regulation and signalling pathways in Merkel cell development. Experimental Dermatology, 30(8), 1051-1064.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

MCC Matched Antibody Pair (723) (CAT#: APMAB-723LY)

MCC Matched Antibody Pair (724) (CAT#: APMAB-724LY)

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MCC Matched Antibody Pair (723) (CAT#: APMAB-723LY)

MCC Matched Antibody Pair (724) (CAT#: APMAB-724LY)

Mouse Anti-MCC (AA 31-130) Recombinant Antibody (CBFYM-0477) (CAT#: CBMAB-M0605-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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