Mouse Anti-MKNK1 Recombinant Antibody (3C9) (CBMAB-A5499-LY)

Name: Mouse Anti-MKNK1 Recombinant Antibody (3C9)
SKU: CBMAB-A5499-LY
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse

Clone

3C9

Antibody Isotype

IgG2a, κ

Application

WB, ELISA

Basic Information

Immunogen

MKNK1 (AAH02755.1, 1 a.a. ~ 465 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human, Mouse

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

MAP Kinase Interacting Serine/Threonine Kinase 1

Entrez Gene ID

Human	8569
Mouse	17346

UniProt ID

Human	Q9BUB5
Mouse	O08605

Alternative Names

MNK1

Function

May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.

Biological Process

Intracellular signal transduction Source: UniProtKB
Peptidyl-serine phosphorylation Source: BHF-UCL
Protein autophosphorylation Source: GO_Central
Protein phosphorylation Source: UniProtKB
Regulation of translation Source: UniProtKB-KW

Cellular Location

Isoform 2:
Cytoplasm
Isoform 3:
Cytoplasm
Nucleus

PTM

Dual phosphorylation of Thr-250 and Thr-255 activates the kinase. Phosphorylation of Thr-385 activates the kinase. MAPK3/ERK1 is one of the kinases which activate MKNK1/MNK1. Phosphorylation by PAK2 leads to a reduced phosphorylation of EIF4G1.

More Infomation

References

Huang, T., Chen, C., Du, J., Zheng, Z., Ye, S., Fang, S., & Liu, K. (2023). A tRF‐5a fragment that regulates radiation resistance of colorectal cancer cells by targeting MKNK1. Journal of Cellular and Molecular Medicine, 27(24), 4021-4033.

Huang, Y., Luo, J., Zhang, Y., Zhang, T., Fei, X., Chen, L., ... & Zhou, J. (2023). Identification of MKNK1 and TOP3A as ovarian endometriosis risk-associated genes using integrative genomic analyses and functional experiments. Computational and Structural Biotechnology Journal, 21, 1510-1522.

Zhang, P., Li, X., Zhang, S., Wu, S., Xiao, Q., Gu, Y., ... & Zhu, L. (2021). miR-370-3p regulates adipogenesis through targeting Mknk1. Molecules, 26(22), 6926.

Fu, M., & Zhang, K. (2021). MAPK interacting serine/threonine kinase 1 (MKNK1), one target gene of miR-223-3p, correlates with neutrophils in sepsis based on bioinformatic analysis. Bioengineered, 12(1), 2550-2562.

Wang, C., Song, J., Liu, W., Yao, Y., Kapranov, P., Sample, K. M., ... & Ben‑David, Y. (2020). FLI1 promotes protein translation via the transcriptional regulation of MKNK1 expression. International Journal of Oncology, 56(2), 430-438.

Liu, K., He, B., Xu, J., Li, Y., Guo, C., Cai, Q., & Wang, S. (2019). miR-483-5p targets MKNK1 to suppress Wilms’ tumor cell proliferation and apoptosis in vitro and in vivo. Medical science monitor: international medical journal of experimental and clinical research, 25, 1459.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Antibody Pairs

MKNK1 Matched Antibody Pair (737)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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