Mouse Anti-SAFB Recombinant Antibody (CBT1120) (V2LY-0625-LY3019)

Name: Mouse Anti-SAFB Recombinant Antibody (CBT1120)
SKU: V2LY-0625-LY3019
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-SAFB Recombinant Antibody (CBT1120) (V2LY-0625-LY3019) in WB

Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

CBT1120

Application

Immunogen

Purified recombinant human SAF-B protein fragments expressed in E.coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:1,000-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Tris-Glycine, Glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

scaffold attachment factor B

Entrez Gene ID

6294

Function

Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing (PubMed:9671816).
Functions as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription (PubMed:12660241).
Thereby acts as a negative regulator of cell proliferation (PubMed:12660241).
When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter (By similarity).

Biological Process

Biological Process chromatin organizationManual Assertion Based On ExperimentTAS:ProtInc
Biological Process intracellular estrogen receptor signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Biological Process regulation of mRNA processingManual Assertion Based On ExperimentIBA:GO_Central
Biological Process regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Nucleus

PTM

Sumoylated by PIAS1 with SUMO1 and SUMO2/3, desumoylated by SENP1. Sumoylation is required for transcriptional repressor activity.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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