Cynomolgus Recombinant CD166 protein, hFc Tag (V2LY-0526-LY1405)

Name: Cynomolgus Recombinant CD166 protein, hFc Tag
SKU: V2LY-0526-LY1405
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Cynomolgus

Tag

hFc Tag

Protein Construction

This product is Cynomolgus Recombinant CD166 protein, hFc Tag consist of Amino Acid: 1-526 and predicts a molecular mass of 82.9 kDa.

Molecule Mass

82.9 kDa

Sequence

Amino Acid: 1-526

Species

Cynomolgus

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Activated Leukocyte Cell Adhesion Molecule

Alternative Names

Activated leukocyte cell adhesion molecule, ALCAM, CD166.

Function

Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts (PubMed:7760007, PubMed:15496415, PubMed:15048703, PubMed:16352806, PubMed:23169771, PubMed:24945728). Promotes T-cell activation and proliferation via its interactions with CD6 (PubMed:15048703, PubMed:16352806, PubMed:24945728). Contributes to the formation and maturation of the immunological synapse via its interactions with CD6 (PubMed:15294938, PubMed:16352806). Mediates homotypic interactions with cells that express ALCAM (PubMed:15496415, PubMed:16352806). Required for normal hematopoietic stem cell engraftment in the bone marrow (PubMed:24740813). Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction (PubMed:23169771). Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions (PubMed:15496415, PubMed:23169771). Required for normal organization of the lymph vessel network. Required for normal hematopoietic stem cell engraftment in the bone marrow. Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow. Promotes in vitro osteoblast proliferation and differentiation (By similarity). Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM. Mediates outgrowth and pathfinding for retinal ganglion cell axons (By similarity).
Isoform 3: Inhibits activities of membrane-bound isoforms by competing for the same interaction partners. Inhibits cell attachment via homotypic interactions. Promotes endothelial cell migration. Inhibits endothelial cell tube formation.

Biological Process

Adaptive immune response Source: UniProtKB-KW
Axon extension involved in axon guidance Source: UniProtKB
Cell adhesion Source: UniProtKB
Heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules Source: UniProtKB
Motor neuron axon guidance Source: Ensembl
Neuron projection extension Source: UniProtKB
Retinal ganglion cell axon guidance Source: UniProtKB
Signal transduction Source: ProtInc

Cellular Location

Cell membrane; Axon; Dendrite. Detected at the immunological synapse, i.e, at the contact zone between antigen-presenting dendritic cells and T-cells (PubMed:15294938, PubMed:16352806). Colocalizes with CD6 and the TCR/CD3 complex at the immunological synapse (PubMed:15294938).
Isoform 3: Secreted

Topology

Extracellular: 28-527 aa
Helical: 528-549 aa
Cytoplasmic: 550-583 aa

PTM

Glycosylated.

Zarghami, N., Soto, M. S., Perez-Balderas, F., Khrapitchev, A. A., Karali, C. S., Johanssen, V. A., ... & Sibson, N. R. (2021). A novel molecular magnetic resonance imaging agent targeting activated leukocyte cell adhesion molecule as demonstrated in mouse brain metastasis models. Journal of Cerebral Blood Flow & Metabolism, 41(7), 1592-1607.

Darvishi, B., Boroumandieh, S., Majidzadeh-A, K., Salehi, M., Jafari, F., & Farahmand, L. (2020). The role of activated leukocyte cell adhesion molecule (ALCAM) in cancer progression, invasion, metastasis and recurrence: A novel cancer stem cell marker and tumor-specific prognostic marker. Experimental and molecular pathology, 115, 104443.

Xie, S., Zhang, H., Wang, F., Liu, Y., Gao, K., Zhang, J., ... & Jiang, W. (2020). Activated leukocyte cell adhesion molecule as a biomarker for disease severity and efficacy of sublingual immunotherapy in allergic rhinitis. International Immunopharmacology, 88, 106975.

Achiha, T., Kijima, N., Kodama, Y., Kagawa, N., Kinoshita, M., Fujimoto, Y., ... & Kishima, H. (2020). Activated leukocyte cell adhesion molecule expression correlates with the WNT subgroup in medulloblastoma and is involved in regulating tumor cell proliferation and invasion. PloS one, 15(12), e0243272.

Sanders, A. J., Owen, S., Morgan, L. D., Ruge, F., Collins, R. J., Ye, L., ... & Jiang, W. G. (2019). Importance of activated leukocyte cell adhesion molecule (ALCAM) in prostate cancer progression and metastatic dissemination. Oncotarget, 10(59), 6362.

Ferragut, F., Cagnoni, A. J., Colombo, L. L., Terrero, C. S., Wolfenstein-Todel, C., Troncoso, M. F., ... & Elola, M. T. (2019). Dual knockdown of Galectin-8 and its glycosylated ligand, the activated leukocyte cell adhesion molecule (ALCAM/CD166), synergistically delays in vivo breast cancer growth. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1866(8), 1338-1352.

Oh, M. S., Hong, J. Y., Kim, M. N., Kwak, E. J., Kim, S. Y., Kim, E. G., ... & Sohn, M. H. (2019). Activated leukocyte cell adhesion molecule modulates Th2 immune response in atopic dermatitis. Allergy, asthma & immunology research, 11(5), 677-690.

Jeong, Y. J., Oh, H. K., Park, S. H., & Bong, J. G. (2018). Prognostic significance of activated leukocyte cell adhesion molecule (ALCAM) in association with promoter methylation of the ALCAM gene in breast cancer. Molecules, 23(1), 131.

Kim, M. N., Hong, J. Y., Shim, D. H., Sol, I. S., Kim, Y. S., Lee, J. H., ... & Sohn, M. H. (2018). Activated leukocyte cell adhesion molecule stimulates the T-cell response in allergic asthma. American journal of respiratory and critical care medicine, 197(8), 994-1008.

Devis, L., Moiola, C. P., Masia, N., Martinez‐Garcia, E., Santacana, M., Stirbat, T. V., ... & Colas, E. (2017). Activated leukocyte cell adhesion molecule (ALCAM) is a marker of recurrence and promotes cell migration, invasion, and metastasis in early‐stage endometrioid endometrial cancer. The Journal of pathology, 241(4), 475-487.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant ALCAM protein, ECD, hFc Tag (CAT#: V2LY-0526-LY2681)

Human Recombinant ALCAM protein, ECD, His Tag (CAT#: V2LY-0526-LY2682)

Mouse Recombinant ALCAM protein, His & hFc Tag (CAT#: V2LY-0526-LY7956)

Mouse Recombinant ALCAM protein, His Tag (CAT#: V2LY-0526-LY7957)

Rat Recombinant ALCAM protein, hFc Tag (CAT#: V2LY-0526-LY9212)

Rat Recombinant ALCAM protein, His Tag (CAT#: V2LY-0526-LY9213)

Cynomolgus Recombinant CD166 protein, His Tag (CAT#: V2LY-0526-LY1406)

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Isotype control

For research use only. Not intended for any clinical use.

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